Experiment: LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06285 and BWI76_RS06290 are separated by 170 nucleotides BWI76_RS06290 and BWI76_RS06295 are separated by 91 nucleotides BWI76_RS06295 and BWI76_RS06300 are separated by 53 nucleotides
BWI76_RS06285: BWI76_RS06285 - peroxiredoxin, at 1,323,566 to 1,324,168
_RS06285
BWI76_RS06290: BWI76_RS06290 - ACP phosphodiesterase, at 1,324,339 to 1,324,920
_RS06290
BWI76_RS06295: BWI76_RS06295 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 1,325,012 to 1,326,082
_RS06295
BWI76_RS06300: BWI76_RS06300 - tRNA guanosine(34) transglycosylase Tgt, at 1,326,136 to 1,327,263
_RS06300
Position (kb)
1325
1326
1327 Strain fitness (log2 ratio)
-1
0
1 at 1324.017 kb on - strand, within BWI76_RS06285 at 1324.044 kb on + strand, within BWI76_RS06285 at 1324.237 kb on - strand at 1324.530 kb on + strand, within BWI76_RS06290 at 1325.098 kb on + strand at 1325.367 kb on + strand, within BWI76_RS06295 at 1325.389 kb on + strand, within BWI76_RS06295 at 1325.503 kb on + strand, within BWI76_RS06295 at 1325.563 kb on - strand, within BWI76_RS06295 at 1325.675 kb on - strand, within BWI76_RS06295 at 1325.827 kb on + strand, within BWI76_RS06295 at 1325.907 kb on + strand, within BWI76_RS06295 at 1326.300 kb on + strand, within BWI76_RS06300 at 1326.781 kb on + strand, within BWI76_RS06300 at 1327.013 kb on - strand, within BWI76_RS06300
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 1,324,017 - BWI76_RS06285 0.75 +0.4 1,324,044 + BWI76_RS06285 0.79 +0.8 1,324,237 - +0.2 1,324,530 + BWI76_RS06290 0.33 +0.1 1,325,098 + +0.1 1,325,367 + BWI76_RS06295 0.33 -0.4 1,325,389 + BWI76_RS06295 0.35 -0.7 1,325,503 + BWI76_RS06295 0.46 -1.5 1,325,563 - BWI76_RS06295 0.51 -1.4 1,325,675 - BWI76_RS06295 0.62 -0.6 1,325,827 + BWI76_RS06295 0.76 -0.0 1,325,907 + BWI76_RS06295 0.84 -0.6 1,326,300 + BWI76_RS06300 0.15 -1.7 1,326,781 + BWI76_RS06300 0.57 -0.0 1,327,013 - BWI76_RS06300 0.78 -0.5
Or see this region's nucleotide sequence