Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22945 and BWI76_RS22950 overlap by 8 nucleotides BWI76_RS22950 and BWI76_RS22955 are separated by 19 nucleotides
BWI76_RS22945: BWI76_RS22945 - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 4,791,213 to 4,792,313
_RS22945
BWI76_RS22950: BWI76_RS22950 - membrane protein, at 4,792,306 to 4,792,701
_RS22950
BWI76_RS22955: BWI76_RS22955 - transcriptional regulator GcvA, at 4,792,721 to 4,793,638
_RS22955
Position (kb)
4792
4793 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4791.324 kb on - strand, within BWI76_RS22945 at 4791.491 kb on + strand, within BWI76_RS22945 at 4791.502 kb on + strand, within BWI76_RS22945 at 4791.636 kb on + strand, within BWI76_RS22945 at 4791.660 kb on + strand, within BWI76_RS22945 at 4792.024 kb on + strand, within BWI76_RS22945 at 4792.229 kb on + strand at 4792.393 kb on - strand, within BWI76_RS22950 at 4792.436 kb on - strand, within BWI76_RS22950 at 4792.704 kb on - strand at 4792.705 kb on - strand at 4792.705 kb on - strand at 4792.805 kb on - strand at 4793.060 kb on + strand, within BWI76_RS22955 at 4793.359 kb on + strand, within BWI76_RS22955 at 4793.402 kb on - strand, within BWI76_RS22955 at 4793.485 kb on + strand, within BWI76_RS22955 at 4793.485 kb on + strand, within BWI76_RS22955 at 4793.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,791,324 - BWI76_RS22945 0.10 +0.0 4,791,491 + BWI76_RS22945 0.25 +0.0 4,791,502 + BWI76_RS22945 0.26 -0.2 4,791,636 + BWI76_RS22945 0.38 -0.7 4,791,660 + BWI76_RS22945 0.41 -2.6 4,792,024 + BWI76_RS22945 0.74 -0.3 4,792,229 + +0.2 4,792,393 - BWI76_RS22950 0.22 -1.1 4,792,436 - BWI76_RS22950 0.33 -3.9 4,792,704 - -0.4 4,792,705 - -0.6 4,792,705 - -0.1 4,792,805 - +0.4 4,793,060 + BWI76_RS22955 0.37 +0.6 4,793,359 + BWI76_RS22955 0.69 +0.6 4,793,402 - BWI76_RS22955 0.74 -1.7 4,793,485 + BWI76_RS22955 0.83 +0.7 4,793,485 + BWI76_RS22955 0.83 +0.2 4,793,636 - -0.4
Or see this region's nucleotide sequence