Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22095 and BWI76_RS22100 are separated by 15 nucleotides BWI76_RS22100 and BWI76_RS22105 are separated by 143 nucleotides
BWI76_RS22095: BWI76_RS22095 - multidrug export protein EmrA, at 4,611,814 to 4,612,986
_RS22095
BWI76_RS22100: BWI76_RS22100 - multidrug effux MFS transporter subunit EmrB, at 4,613,002 to 4,614,540
_RS22100
BWI76_RS22105: BWI76_RS22105 - 2-isopropylmalate synthase, at 4,614,684 to 4,616,360
_RS22105
Position (kb)
4613
4614
4615 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4612.093 kb on - strand at 4612.204 kb on - strand, within BWI76_RS22095 at 4612.390 kb on - strand, within BWI76_RS22095 at 4612.997 kb on + strand at 4613.131 kb on - strand at 4613.221 kb on - strand, within BWI76_RS22100 at 4613.901 kb on - strand, within BWI76_RS22100 at 4614.041 kb on + strand, within BWI76_RS22100 at 4614.109 kb on + strand, within BWI76_RS22100 at 4614.287 kb on + strand, within BWI76_RS22100 at 4614.608 kb on + strand at 4614.689 kb on + strand at 4615.021 kb on - strand, within BWI76_RS22105 at 4615.343 kb on + strand, within BWI76_RS22105 at 4615.344 kb on - strand, within BWI76_RS22105 at 4615.344 kb on - strand, within BWI76_RS22105 at 4615.351 kb on - strand, within BWI76_RS22105 at 4615.351 kb on - strand, within BWI76_RS22105
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,612,093 - +2.1 4,612,204 - BWI76_RS22095 0.33 -1.1 4,612,390 - BWI76_RS22095 0.49 -1.1 4,612,997 + +1.1 4,613,131 - -1.9 4,613,221 - BWI76_RS22100 0.14 -0.4 4,613,901 - BWI76_RS22100 0.58 -0.7 4,614,041 + BWI76_RS22100 0.68 -0.7 4,614,109 + BWI76_RS22100 0.72 +0.2 4,614,287 + BWI76_RS22100 0.83 -1.8 4,614,608 + -0.6 4,614,689 + -0.9 4,615,021 - BWI76_RS22105 0.20 +0.6 4,615,343 + BWI76_RS22105 0.39 +0.5 4,615,344 - BWI76_RS22105 0.39 +0.4 4,615,344 - BWI76_RS22105 0.39 -0.2 4,615,351 - BWI76_RS22105 0.40 +0.2 4,615,351 - BWI76_RS22105 0.40 -1.5
Or see this region's nucleotide sequence