Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21440

Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21435 and BWI76_RS21440 are separated by 451 nucleotidesBWI76_RS21440 and BWI76_RS21445 are separated by 127 nucleotidesBWI76_RS21445 and BWI76_RS21450 overlap by 4 nucleotides BWI76_RS21435: BWI76_RS21435 - 16S ribosomal RNA, at 4,488,438 to 4,489,991 _RS21435 BWI76_RS21440: BWI76_RS21440 - ATP-dependent chaperone ClpB, at 4,490,443 to 4,493,016 _RS21440 BWI76_RS21445: BWI76_RS21445 - hypothetical protein, at 4,493,144 to 4,493,875 _RS21445 BWI76_RS21450: BWI76_RS21450 - 23S rRNA pseudouridine(1911/1915/1917) synthase, at 4,493,872 to 4,494,852 _RS21450 Position (kb) 4490 4491 4492 4493 4494Strain fitness (log2 ratio) -2 -1 0 1at 4490.289 kb on + strandat 4490.366 kb on + strandat 4490.443 kb on + strandat 4490.709 kb on + strandat 4490.821 kb on - strand, within BWI76_RS21440at 4490.826 kb on + strand, within BWI76_RS21440at 4490.959 kb on - strand, within BWI76_RS21440at 4491.100 kb on - strand, within BWI76_RS21440at 4491.129 kb on + strandat 4491.175 kb on - strand, within BWI76_RS21440at 4491.200 kb on - strand, within BWI76_RS21440at 4491.327 kb on + strand, within BWI76_RS21440at 4491.455 kb on + strand, within BWI76_RS21440at 4491.751 kb on + strand, within BWI76_RS21440at 4491.962 kb on - strand, within BWI76_RS21440at 4491.984 kb on + strand, within BWI76_RS21440at 4492.194 kb on + strand, within BWI76_RS21440at 4492.326 kb on - strand, within BWI76_RS21440at 4492.487 kb on + strand, within BWI76_RS21440at 4492.487 kb on + strand, within BWI76_RS21440at 4492.487 kb on + strand, within BWI76_RS21440at 4492.487 kb on + strand, within BWI76_RS21440at 4492.487 kb on + strand, within BWI76_RS21440at 4492.495 kb on - strand, within BWI76_RS21440at 4492.495 kb on - strand, within BWI76_RS21440at 4492.495 kb on - strand, within BWI76_RS21440at 4492.495 kb on - strand, within BWI76_RS21440at 4492.495 kb on - strand, within BWI76_RS21440at 4492.495 kb on - strand, within BWI76_RS21440at 4492.495 kb on - strand, within BWI76_RS21440at 4492.627 kb on - strand, within BWI76_RS21440at 4492.740 kb on + strand, within BWI76_RS21440at 4492.740 kb on + strand, within BWI76_RS21440at 4492.748 kb on - strand, within BWI76_RS21440at 4492.748 kb on - strand, within BWI76_RS21440at 4492.748 kb on - strand, within BWI76_RS21440at 4492.798 kb on - strandat 4492.798 kb on - strandat 4492.818 kb on - strandat 4492.920 kb on - strandat 4492.973 kb on - strandat 4492.985 kb on - strandat 4493.082 kb on + strandat 4493.090 kb on + strandat 4493.090 kb on + strandat 4493.098 kb on - strandat 4493.142 kb on + strandat 4493.156 kb on + strandat 4493.232 kb on - strand, within BWI76_RS21445at 4493.386 kb on + strand, within BWI76_RS21445at 4493.413 kb on + strand, within BWI76_RS21445

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
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4,490,289 + -0.3
4,490,366 + -0.3
4,490,443 + -0.3
4,490,709 + -0.1
4,490,821 - BWI76_RS21440 0.15 -0.6
4,490,826 + BWI76_RS21440 0.15 -1.0
4,490,959 - BWI76_RS21440 0.20 -0.8
4,491,100 - BWI76_RS21440 0.26 -0.9
4,491,129 + +1.4
4,491,175 - BWI76_RS21440 0.28 +0.1
4,491,200 - BWI76_RS21440 0.29 -1.9
4,491,327 + BWI76_RS21440 0.34 -0.9
4,491,455 + BWI76_RS21440 0.39 +0.1
4,491,751 + BWI76_RS21440 0.51 +0.2
4,491,962 - BWI76_RS21440 0.59 -0.2
4,491,984 + BWI76_RS21440 0.60 -2.1
4,492,194 + BWI76_RS21440 0.68 -0.4
4,492,326 - BWI76_RS21440 0.73 -0.2
4,492,487 + BWI76_RS21440 0.79 +0.5
4,492,487 + BWI76_RS21440 0.79 -0.9
4,492,487 + BWI76_RS21440 0.79 -0.4
4,492,487 + BWI76_RS21440 0.79 -0.9
4,492,487 + BWI76_RS21440 0.79 -0.5
4,492,495 - BWI76_RS21440 0.80 -1.3
4,492,495 - BWI76_RS21440 0.80 -0.4
4,492,495 - BWI76_RS21440 0.80 -0.9
4,492,495 - BWI76_RS21440 0.80 -1.9
4,492,495 - BWI76_RS21440 0.80 -0.9
4,492,495 - BWI76_RS21440 0.80 -0.8
4,492,495 - BWI76_RS21440 0.80 -0.2
4,492,627 - BWI76_RS21440 0.85 -0.5
4,492,740 + BWI76_RS21440 0.89 +1.5
4,492,740 + BWI76_RS21440 0.89 -1.5
4,492,748 - BWI76_RS21440 0.90 -0.8
4,492,748 - BWI76_RS21440 0.90 +0.1
4,492,748 - BWI76_RS21440 0.90 -2.4
4,492,798 - -0.1
4,492,798 - +0.2
4,492,818 - -1.7
4,492,920 - -1.1
4,492,973 - -0.7
4,492,985 - +0.1
4,493,082 + -0.8
4,493,090 + -0.6
4,493,090 + -0.1
4,493,098 - -0.6
4,493,142 + +0.2
4,493,156 + +0.9
4,493,232 - BWI76_RS21445 0.12 +0.6
4,493,386 + BWI76_RS21445 0.33 +0.0
4,493,413 + BWI76_RS21445 0.37 +0.1

Or see this region's nucleotide sequence