Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21065 and BWI76_RS21070 are separated by 114 nucleotides BWI76_RS21070 and BWI76_RS21075 are separated by 10 nucleotides BWI76_RS21075 and BWI76_RS21080 are separated by 34 nucleotides
BWI76_RS21065: BWI76_RS21065 - ribosome biogenesis GTPase Der, at 4,406,446 to 4,407,924
_RS21065
BWI76_RS21070: BWI76_RS21070 - outer membrane protein assembly factor BamB, at 4,408,039 to 4,409,217
_RS21070
BWI76_RS21075: BWI76_RS21075 - membrane protein, at 4,409,228 to 4,409,848
_RS21075
BWI76_RS21080: BWI76_RS21080 - histidine--tRNA ligase, at 4,409,883 to 4,411,157
_RS21080
Position (kb) 4408
4409
4410 Strain fitness (log2 ratio) -2
-1
0
1 at 4408.334 kb on - strand, within BWI76_RS21070 at 4408.461 kb on - strand, within BWI76_RS21070 at 4409.039 kb on - strand, within BWI76_RS21070 at 4409.392 kb on - strand, within BWI76_RS21075 at 4409.438 kb on - strand, within BWI76_RS21075 at 4409.713 kb on - strand, within BWI76_RS21075 at 4409.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,408,334 - BWI76_RS21070 0.25 -2.1 4,408,461 - BWI76_RS21070 0.36 -1.2 4,409,039 - BWI76_RS21070 0.85 -2.0 4,409,392 - BWI76_RS21075 0.26 -0.5 4,409,438 - BWI76_RS21075 0.34 +0.9 4,409,713 - BWI76_RS21075 0.78 +1.1 4,409,894 - -0.3
Or see this region's nucleotide sequence