Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15025

Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15020 and BWI76_RS15025 are separated by 29 nucleotidesBWI76_RS15025 and BWI76_RS15030 overlap by 4 nucleotidesBWI76_RS15030 and BWI76_RS15035 overlap by 16 nucleotides BWI76_RS15020: BWI76_RS15020 - 2-oxobutyrate oxidase, at 3,144,595 to 3,145,623 _RS15020 BWI76_RS15025: BWI76_RS15025 - NLPA lipoprotein, at 3,145,653 to 3,146,459 _RS15025 BWI76_RS15030: BWI76_RS15030 - ABC transporter, at 3,146,456 to 3,147,247 _RS15030 BWI76_RS15035: BWI76_RS15035 - metal ABC transporter permease, at 3,147,232 to 3,147,903 _RS15035 Position (kb) 3145 3146 3147Strain fitness (log2 ratio) -2 -1 0 1at 3144.730 kb on - strand, within BWI76_RS15020at 3144.923 kb on - strand, within BWI76_RS15020at 3145.071 kb on + strand, within BWI76_RS15020at 3145.186 kb on + strand, within BWI76_RS15020at 3145.415 kb on + strand, within BWI76_RS15020at 3145.449 kb on - strand, within BWI76_RS15020at 3145.681 kb on + strandat 3145.689 kb on - strandat 3146.294 kb on - strand, within BWI76_RS15025at 3146.888 kb on - strand, within BWI76_RS15030at 3147.155 kb on - strand, within BWI76_RS15030at 3147.407 kb on - strand, within BWI76_RS15035

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
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3,144,730 - BWI76_RS15020 0.13 +0.4
3,144,923 - BWI76_RS15020 0.32 +1.1
3,145,071 + BWI76_RS15020 0.46 +0.0
3,145,186 + BWI76_RS15020 0.57 -0.4
3,145,415 + BWI76_RS15020 0.80 +0.2
3,145,449 - BWI76_RS15020 0.83 +0.1
3,145,681 + +1.5
3,145,689 - +0.7
3,146,294 - BWI76_RS15025 0.79 +0.3
3,146,888 - BWI76_RS15030 0.55 +0.1
3,147,155 - BWI76_RS15030 0.88 +0.4
3,147,407 - BWI76_RS15035 0.26 -1.9

Or see this region's nucleotide sequence