Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12850 and BWI76_RS12855 are separated by 23 nucleotides BWI76_RS12855 and BWI76_RS12860 overlap by 4 nucleotides BWI76_RS12860 and BWI76_RS12865 overlap by 8 nucleotides
BWI76_RS12850: BWI76_RS12850 - hypothetical protein, at 2,678,472 to 2,679,731
_RS12850
BWI76_RS12855: BWI76_RS12855 - enamine deaminase RidA, at 2,679,755 to 2,680,189
_RS12855
BWI76_RS12860: BWI76_RS12860 - hypothetical protein, at 2,680,186 to 2,680,860
_RS12860
BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022
_RS12865
Position (kb)
2680
2681 Strain fitness (log2 ratio)
-1
0
1 at 2679.228 kb on - strand, within BWI76_RS12850 at 2679.241 kb on + strand, within BWI76_RS12850 at 2679.249 kb on - strand, within BWI76_RS12850 at 2679.347 kb on + strand, within BWI76_RS12850 at 2679.389 kb on - strand, within BWI76_RS12850 at 2679.769 kb on - strand at 2679.837 kb on - strand, within BWI76_RS12855 at 2680.044 kb on + strand, within BWI76_RS12855 at 2680.303 kb on + strand, within BWI76_RS12860 at 2680.518 kb on - strand, within BWI76_RS12860 at 2681.019 kb on - strand, within BWI76_RS12865 at 2681.110 kb on - strand, within BWI76_RS12865 at 2681.268 kb on + strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.518 kb on - strand, within BWI76_RS12865
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 2,679,228 - BWI76_RS12850 0.60 -0.1 2,679,241 + BWI76_RS12850 0.61 -0.0 2,679,249 - BWI76_RS12850 0.62 +0.5 2,679,347 + BWI76_RS12850 0.69 +0.3 2,679,389 - BWI76_RS12850 0.73 -0.1 2,679,769 - -1.1 2,679,837 - BWI76_RS12855 0.19 +0.9 2,680,044 + BWI76_RS12855 0.66 +0.2 2,680,303 + BWI76_RS12860 0.17 -1.0 2,680,518 - BWI76_RS12860 0.49 -0.8 2,681,019 - BWI76_RS12865 0.14 +0.7 2,681,110 - BWI76_RS12865 0.22 +0.1 2,681,268 + BWI76_RS12865 0.35 +0.0 2,681,368 - BWI76_RS12865 0.44 -0.6 2,681,368 - BWI76_RS12865 0.44 -0.3 2,681,518 - BWI76_RS12865 0.57 -0.3
Or see this region's nucleotide sequence