Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08975 and BWI76_RS08980 are separated by 376 nucleotides BWI76_RS08980 and BWI76_RS08985 are separated by 221 nucleotides
BWI76_RS08975: BWI76_RS08975 - amino acid ABC transporter substrate-binding protein, at 1,891,396 to 1,892,142
_RS08975
BWI76_RS08980: BWI76_RS08980 - DNA starvation/stationary phase protection protein, at 1,892,519 to 1,893,022
_RS08980
BWI76_RS08985: BWI76_RS08985 - threonine/homoserine exporter RhtA, at 1,893,244 to 1,894,131
_RS08985
Position (kb)
1892
1893
1894 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1891.553 kb on + strand, within BWI76_RS08975 at 1891.944 kb on + strand, within BWI76_RS08975 at 1891.981 kb on + strand, within BWI76_RS08975 at 1891.994 kb on - strand, within BWI76_RS08975 at 1892.126 kb on - strand at 1892.145 kb on - strand at 1892.167 kb on + strand at 1892.167 kb on + strand at 1892.449 kb on + strand at 1892.457 kb on - strand at 1892.524 kb on - strand at 1892.683 kb on - strand, within BWI76_RS08980 at 1893.108 kb on + strand at 1893.299 kb on - strand at 1893.461 kb on + strand, within BWI76_RS08985 at 1893.469 kb on - strand, within BWI76_RS08985 at 1893.586 kb on + strand, within BWI76_RS08985 at 1893.950 kb on + strand, within BWI76_RS08985
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 1,891,553 + BWI76_RS08975 0.21 -0.7 1,891,944 + BWI76_RS08975 0.73 -0.2 1,891,981 + BWI76_RS08975 0.78 +2.1 1,891,994 - BWI76_RS08975 0.80 +0.1 1,892,126 - +0.1 1,892,145 - -1.4 1,892,167 + +0.2 1,892,167 + +3.1 1,892,449 + -0.6 1,892,457 - -0.7 1,892,524 - +0.8 1,892,683 - BWI76_RS08980 0.33 -1.6 1,893,108 + -0.3 1,893,299 - +0.3 1,893,461 + BWI76_RS08985 0.24 -0.1 1,893,469 - BWI76_RS08985 0.25 +0.6 1,893,586 + BWI76_RS08985 0.39 -1.3 1,893,950 + BWI76_RS08985 0.80 -0.5
Or see this region's nucleotide sequence