Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08715 and BWI76_RS08720 are separated by 230 nucleotides BWI76_RS08720 and BWI76_RS08725 overlap by 4 nucleotides
BWI76_RS08715: BWI76_RS08715 - acyl-CoA thioesterase, at 1,835,092 to 1,836,375
_RS08715
BWI76_RS08720: BWI76_RS08720 - imidazolonepropionase, at 1,836,606 to 1,837,823
_RS08720
BWI76_RS08725: BWI76_RS08725 - Formiminoglutamase (EC 3.5.3.8) (from data) , at 1,837,820 to 1,838,776
_RS08725
Position (kb)
1836
1837
1838 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1835.711 kb on - strand, within BWI76_RS08715 at 1835.788 kb on + strand, within BWI76_RS08715 at 1836.021 kb on + strand, within BWI76_RS08715 at 1836.029 kb on - strand, within BWI76_RS08715 at 1836.096 kb on + strand, within BWI76_RS08715 at 1836.330 kb on - strand at 1836.334 kb on + strand at 1836.434 kb on - strand at 1836.585 kb on + strand at 1836.688 kb on - strand at 1836.688 kb on - strand at 1837.299 kb on + strand, within BWI76_RS08720 at 1837.602 kb on + strand, within BWI76_RS08720 at 1837.653 kb on - strand, within BWI76_RS08720 at 1837.885 kb on - strand at 1838.181 kb on + strand, within BWI76_RS08725 at 1838.229 kb on + strand, within BWI76_RS08725 at 1838.484 kb on - strand, within BWI76_RS08725 at 1838.668 kb on + strand, within BWI76_RS08725 at 1838.668 kb on + strand, within BWI76_RS08725
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 1,835,711 - BWI76_RS08715 0.48 +0.7 1,835,788 + BWI76_RS08715 0.54 +0.0 1,836,021 + BWI76_RS08715 0.72 -0.9 1,836,029 - BWI76_RS08715 0.73 +0.4 1,836,096 + BWI76_RS08715 0.78 +0.3 1,836,330 - -0.3 1,836,334 + +0.4 1,836,434 - +0.4 1,836,585 + -0.0 1,836,688 - -0.3 1,836,688 - +2.2 1,837,299 + BWI76_RS08720 0.57 -2.4 1,837,602 + BWI76_RS08720 0.82 -1.3 1,837,653 - BWI76_RS08720 0.86 +0.1 1,837,885 - -0.5 1,838,181 + BWI76_RS08725 0.38 -0.3 1,838,229 + BWI76_RS08725 0.43 +0.3 1,838,484 - BWI76_RS08725 0.69 -0.1 1,838,668 + BWI76_RS08725 0.89 -0.5 1,838,668 + BWI76_RS08725 0.89 -0.2
Or see this region's nucleotide sequence