Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01120 and BWI76_RS01125 overlap by 4 nucleotides BWI76_RS01125 and BWI76_RS01130 overlap by 4 nucleotides
BWI76_RS01120: BWI76_RS01120 - O-antigen translocase, at 230,576 to 231,826
_RS01120
BWI76_RS01125: BWI76_RS01125 - 4-alpha-L-fucosyltransferase, at 231,823 to 232,902
_RS01125
BWI76_RS01130: BWI76_RS01130 - enterobacterial common antigen polymerase, at 232,899 to 234,245
_RS01130
Position (kb) 231
232
233 Strain fitness (log2 ratio) -2
-1
0
1 at 230.926 kb on - strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 230.953 kb on + strand, within BWI76_RS01120 at 231.208 kb on + strand, within BWI76_RS01120 at 231.388 kb on + strand, within BWI76_RS01120 at 231.410 kb on + strand, within BWI76_RS01120 at 231.627 kb on - strand, within BWI76_RS01120 at 231.640 kb on + strand, within BWI76_RS01120 at 231.739 kb on - strand at 231.759 kb on - strand at 231.759 kb on - strand at 231.787 kb on + strand at 231.795 kb on - strand at 231.824 kb on + strand at 231.832 kb on - strand at 231.877 kb on + strand at 231.898 kb on + strand at 232.139 kb on - strand, within BWI76_RS01125 at 232.219 kb on - strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.534 kb on + strand, within BWI76_RS01125 at 232.542 kb on - strand, within BWI76_RS01125 at 232.712 kb on - strand, within BWI76_RS01125 at 232.727 kb on + strand, within BWI76_RS01125 at 232.768 kb on + strand, within BWI76_RS01125 at 232.795 kb on - strand at 232.839 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 230,926 - BWI76_RS01120 0.28 -1.3 230,953 + BWI76_RS01120 0.30 +0.8 230,953 + BWI76_RS01120 0.30 -0.3 231,208 + BWI76_RS01120 0.51 -0.1 231,388 + BWI76_RS01120 0.65 +0.8 231,410 + BWI76_RS01120 0.67 -1.4 231,627 - BWI76_RS01120 0.84 -2.0 231,640 + BWI76_RS01120 0.85 +1.1 231,739 - +1.1 231,759 - -0.7 231,759 - -0.2 231,787 + -0.4 231,795 - -0.5 231,824 + -0.6 231,832 - -0.7 231,877 + -1.1 231,898 + -1.6 232,139 - BWI76_RS01125 0.29 -0.8 232,219 - BWI76_RS01125 0.37 -1.6 232,534 + BWI76_RS01125 0.66 -1.5 232,534 + BWI76_RS01125 0.66 +0.2 232,534 + BWI76_RS01125 0.66 -0.7 232,542 - BWI76_RS01125 0.67 -1.1 232,712 - BWI76_RS01125 0.82 -2.4 232,727 + BWI76_RS01125 0.84 -0.8 232,768 + BWI76_RS01125 0.88 -1.6 232,795 - -1.6 232,839 - -1.3
Or see this region's nucleotide sequence