Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01085 and BWI76_RS01090 are separated by 56 nucleotides BWI76_RS01090 and BWI76_RS01095 overlap by 4 nucleotides
BWI76_RS01085: BWI76_RS01085 - polysaccharide chain length modulation protein, at 223,330 to 224,376
_RS01085
BWI76_RS01090: BWI76_RS01090 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 224,433 to 225,563
_RS01090
BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822
_RS01095
Position (kb) 224
225
226 Strain fitness (log2 ratio) -2
-1
0
1 at 223.438 kb on + strand, within BWI76_RS01085 at 223.608 kb on + strand, within BWI76_RS01085 at 223.697 kb on + strand, within BWI76_RS01085 at 223.917 kb on + strand, within BWI76_RS01085 at 224.006 kb on + strand, within BWI76_RS01085 at 224.069 kb on + strand, within BWI76_RS01085 at 224.097 kb on + strand, within BWI76_RS01085 at 224.119 kb on - strand, within BWI76_RS01085 at 224.184 kb on - strand, within BWI76_RS01085 at 224.637 kb on - strand, within BWI76_RS01090 at 224.826 kb on - strand, within BWI76_RS01090 at 224.859 kb on + strand, within BWI76_RS01090 at 225.000 kb on - strand, within BWI76_RS01090 at 225.010 kb on + strand, within BWI76_RS01090 at 225.020 kb on + strand, within BWI76_RS01090 at 225.502 kb on + strand at 225.589 kb on + strand at 226.034 kb on - strand, within BWI76_RS01095 at 226.533 kb on + strand, within BWI76_RS01095 at 226.541 kb on - strand, within BWI76_RS01095
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 223,438 + BWI76_RS01085 0.10 -0.8 223,608 + BWI76_RS01085 0.27 -0.8 223,697 + BWI76_RS01085 0.35 -2.3 223,917 + BWI76_RS01085 0.56 -1.5 224,006 + BWI76_RS01085 0.65 -1.1 224,069 + BWI76_RS01085 0.71 -2.5 224,097 + BWI76_RS01085 0.73 -1.1 224,119 - BWI76_RS01085 0.75 +0.7 224,184 - BWI76_RS01085 0.82 -0.8 224,637 - BWI76_RS01090 0.18 +0.2 224,826 - BWI76_RS01090 0.35 -2.5 224,859 + BWI76_RS01090 0.38 -1.7 225,000 - BWI76_RS01090 0.50 -2.3 225,010 + BWI76_RS01090 0.51 -1.5 225,020 + BWI76_RS01090 0.52 -1.0 225,502 + -1.3 225,589 + -2.2 226,034 - BWI76_RS01095 0.38 -1.6 226,533 + BWI76_RS01095 0.77 -1.3 226,541 - BWI76_RS01095 0.78 -1.3
Or see this region's nucleotide sequence