Experiment: Varel_Bryant_medium_Glucose with Sulfamonomethoxine 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1622 and BT1623 are separated by 166 nucleotides BT1623 and BT1624 are separated by 134 nucleotides
BT1622: BT1622 - N-acetylgalactosamine-6-sulfatase precursor (NCBI ptt file), at 1,996,551 to 1,998,053
BT1622
BT1623: BT1623 - putative membrane protein (NCBI ptt file), at 1,998,220 to 1,999,395
BT1623
BT1624: BT1624 - putative secreted sulfatase (NCBI ptt file), at 1,999,530 to 2,001,077
BT1624
Position (kb)
1998
1999
2000 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1997.220 kb on + strand, within BT1622 at 1997.352 kb on + strand, within BT1622 at 1997.430 kb on + strand, within BT1622 at 1997.430 kb on + strand, within BT1622 at 1997.430 kb on + strand, within BT1622 at 1997.526 kb on - strand, within BT1622 at 1997.830 kb on - strand, within BT1622 at 1997.834 kb on - strand, within BT1622 at 1997.836 kb on - strand, within BT1622 at 1997.941 kb on - strand at 1998.033 kb on - strand at 1998.067 kb on + strand at 1998.140 kb on + strand at 1998.140 kb on + strand at 1998.141 kb on - strand at 1998.237 kb on - strand at 1998.321 kb on + strand at 1998.338 kb on + strand, within BT1623 at 1998.360 kb on - strand, within BT1623 at 1998.441 kb on - strand, within BT1623 at 1998.573 kb on + strand, within BT1623 at 1998.648 kb on + strand, within BT1623 at 1998.650 kb on + strand, within BT1623 at 1998.774 kb on + strand, within BT1623 at 1998.892 kb on + strand, within BT1623 at 1999.058 kb on + strand, within BT1623 at 1999.223 kb on + strand, within BT1623 at 1999.224 kb on - strand, within BT1623 at 1999.295 kb on - strand at 1999.391 kb on - strand at 1999.396 kb on - strand at 1999.408 kb on + strand at 1999.408 kb on + strand at 1999.409 kb on - strand at 1999.411 kb on - strand at 1999.412 kb on + strand at 1999.511 kb on + strand at 1999.519 kb on - strand at 1999.685 kb on + strand, within BT1624 at 1999.974 kb on - strand, within BT1624 at 2000.114 kb on + strand, within BT1624 at 2000.114 kb on + strand, within BT1624 at 2000.120 kb on + strand, within BT1624 at 2000.378 kb on + strand, within BT1624 at 2000.380 kb on + strand, within BT1624
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfamonomethoxine 0.2 mM remove 1,997,220 + BT1622 0.45 -2.2 1,997,352 + BT1622 0.53 -1.5 1,997,430 + BT1622 0.58 -2.5 1,997,430 + BT1622 0.58 -1.9 1,997,430 + BT1622 0.58 +0.1 1,997,526 - BT1622 0.65 -1.7 1,997,830 - BT1622 0.85 +0.1 1,997,834 - BT1622 0.85 +0.1 1,997,836 - BT1622 0.85 -0.9 1,997,941 - +0.8 1,998,033 - -1.0 1,998,067 + -0.8 1,998,140 + +0.1 1,998,140 + -1.6 1,998,141 - -0.9 1,998,237 - -1.5 1,998,321 + -1.7 1,998,338 + BT1623 0.10 -2.0 1,998,360 - BT1623 0.12 -2.3 1,998,441 - BT1623 0.19 +0.1 1,998,573 + BT1623 0.30 -1.5 1,998,648 + BT1623 0.36 +0.1 1,998,650 + BT1623 0.37 +0.1 1,998,774 + BT1623 0.47 -1.3 1,998,892 + BT1623 0.57 -1.9 1,999,058 + BT1623 0.71 -1.8 1,999,223 + BT1623 0.85 -3.1 1,999,224 - BT1623 0.85 -1.9 1,999,295 - -1.7 1,999,391 - -2.3 1,999,396 - -1.9 1,999,408 + -1.2 1,999,408 + +0.1 1,999,409 - -1.9 1,999,411 - +0.1 1,999,412 + +1.1 1,999,511 + -0.9 1,999,519 - -0.2 1,999,685 + BT1624 0.10 -1.5 1,999,974 - BT1624 0.29 +1.7 2,000,114 + BT1624 0.38 -2.6 2,000,114 + BT1624 0.38 -0.1 2,000,120 + BT1624 0.38 -0.9 2,000,378 + BT1624 0.55 +0.1 2,000,380 + BT1624 0.55 -2.6
Or see this region's nucleotide sequence