Experiment: Suberic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21410 and RR42_RS21415 are separated by 144 nucleotides RR42_RS21415 and RR42_RS21420 are separated by 160 nucleotides
RR42_RS21410: RR42_RS21410 - DNA-binding protein, at 133,775 to 134,095
_RS21410
RR42_RS21415: RR42_RS21415 - hypothetical protein, at 134,240 to 134,638
_RS21415
RR42_RS21420: RR42_RS21420 - acyl-CoA dehydrogenase, at 134,799 to 135,956
_RS21420
Position (kb) 133
134
135 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 132.831 kb on + strand at 132.860 kb on + strand at 132.861 kb on - strand at 132.928 kb on + strand at 132.929 kb on - strand at 132.929 kb on - strand at 133.070 kb on + strand at 133.229 kb on + strand at 133.230 kb on - strand at 133.275 kb on + strand at 133.275 kb on + strand at 133.407 kb on + strand at 133.407 kb on + strand at 133.407 kb on + strand at 133.408 kb on - strand at 133.541 kb on + strand at 133.542 kb on - strand at 133.543 kb on + strand at 133.543 kb on + strand at 133.543 kb on + strand at 133.543 kb on + strand at 133.544 kb on - strand at 133.544 kb on - strand at 133.544 kb on - strand at 133.544 kb on - strand at 133.551 kb on + strand at 133.551 kb on + strand at 133.673 kb on - strand at 133.720 kb on + strand at 133.721 kb on - strand at 133.784 kb on + strand at 133.784 kb on + strand at 133.785 kb on - strand at 133.861 kb on + strand, within RR42_RS21410 at 133.862 kb on - strand, within RR42_RS21410 at 133.862 kb on - strand, within RR42_RS21410 at 133.892 kb on + strand, within RR42_RS21410 at 133.892 kb on + strand, within RR42_RS21410 at 133.892 kb on + strand, within RR42_RS21410 at 133.893 kb on - strand, within RR42_RS21410 at 133.893 kb on - strand, within RR42_RS21410 at 133.997 kb on + strand, within RR42_RS21410 at 134.060 kb on - strand, within RR42_RS21410 at 134.321 kb on + strand, within RR42_RS21415 at 134.321 kb on + strand, within RR42_RS21415 at 134.420 kb on + strand, within RR42_RS21415 at 134.420 kb on + strand, within RR42_RS21415 at 134.421 kb on - strand, within RR42_RS21415 at 134.421 kb on - strand, within RR42_RS21415 at 134.422 kb on + strand, within RR42_RS21415 at 134.422 kb on + strand, within RR42_RS21415 at 134.423 kb on - strand, within RR42_RS21415 at 134.423 kb on - strand, within RR42_RS21415 at 134.423 kb on - strand, within RR42_RS21415 at 134.423 kb on - strand, within RR42_RS21415 at 134.606 kb on - strand at 134.707 kb on - strand at 134.755 kb on + strand at 134.755 kb on + strand at 134.755 kb on + strand at 134.755 kb on + strand at 134.755 kb on + strand at 134.755 kb on + strand at 134.755 kb on + strand at 134.755 kb on + strand at 134.756 kb on - strand at 134.756 kb on - strand at 134.756 kb on - strand at 134.756 kb on - strand at 134.756 kb on - strand at 134.970 kb on + strand, within RR42_RS21420 at 134.970 kb on + strand, within RR42_RS21420 at 134.971 kb on - strand, within RR42_RS21420 at 134.971 kb on - strand, within RR42_RS21420 at 134.971 kb on - strand, within RR42_RS21420 at 135.015 kb on + strand, within RR42_RS21420 at 135.015 kb on + strand, within RR42_RS21420 at 135.015 kb on + strand, within RR42_RS21420 at 135.016 kb on - strand, within RR42_RS21420 at 135.016 kb on - strand, within RR42_RS21420 at 135.016 kb on - strand, within RR42_RS21420
Per-strain Table
Position Strand Gene LocusTag Fraction Suberic (C) remove 132,831 + +1.6 132,860 + +0.6 132,861 - +0.8 132,928 + -0.4 132,929 - -2.4 132,929 - +1.6 133,070 + +1.6 133,229 + +0.7 133,230 - +0.2 133,275 + +1.6 133,275 + -0.8 133,407 + +0.6 133,407 + -0.0 133,407 + -1.2 133,408 - -0.0 133,541 + +0.8 133,542 - -0.6 133,543 + +1.0 133,543 + +0.7 133,543 + +0.7 133,543 + -0.8 133,544 - +0.5 133,544 - -1.0 133,544 - -0.4 133,544 - +2.1 133,551 + -0.5 133,551 + +0.4 133,673 - +0.5 133,720 + +0.4 133,721 - +1.7 133,784 + +0.1 133,784 + -0.1 133,785 - +0.7 133,861 + RR42_RS21410 0.27 +0.9 133,862 - RR42_RS21410 0.27 -0.3 133,862 - RR42_RS21410 0.27 -0.6 133,892 + RR42_RS21410 0.36 -0.4 133,892 + RR42_RS21410 0.36 +0.2 133,892 + RR42_RS21410 0.36 -0.1 133,893 - RR42_RS21410 0.37 +1.6 133,893 - RR42_RS21410 0.37 +0.0 133,997 + RR42_RS21410 0.69 -0.5 134,060 - RR42_RS21410 0.89 +1.3 134,321 + RR42_RS21415 0.20 +1.2 134,321 + RR42_RS21415 0.20 +0.1 134,420 + RR42_RS21415 0.45 +1.7 134,420 + RR42_RS21415 0.45 -0.2 134,421 - RR42_RS21415 0.45 +3.0 134,421 - RR42_RS21415 0.45 -0.6 134,422 + RR42_RS21415 0.46 -0.3 134,422 + RR42_RS21415 0.46 -0.7 134,423 - RR42_RS21415 0.46 -0.9 134,423 - RR42_RS21415 0.46 +1.7 134,423 - RR42_RS21415 0.46 -0.5 134,423 - RR42_RS21415 0.46 -0.2 134,606 - -1.3 134,707 - -2.7 134,755 + -1.7 134,755 + -2.4 134,755 + -0.3 134,755 + -2.0 134,755 + -0.9 134,755 + +0.1 134,755 + -2.3 134,755 + +0.9 134,756 - -1.5 134,756 - -1.9 134,756 - +1.1 134,756 - -2.2 134,756 - -4.0 134,970 + RR42_RS21420 0.15 -1.5 134,970 + RR42_RS21420 0.15 -3.5 134,971 - RR42_RS21420 0.15 -3.8 134,971 - RR42_RS21420 0.15 -1.9 134,971 - RR42_RS21420 0.15 -2.5 135,015 + RR42_RS21420 0.19 -2.9 135,015 + RR42_RS21420 0.19 -0.3 135,015 + RR42_RS21420 0.19 -2.5 135,016 - RR42_RS21420 0.19 -0.6 135,016 - RR42_RS21420 0.19 -4.7 135,016 - RR42_RS21420 0.19 -0.9
Or see this region's nucleotide sequence