Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c15260 and PGA1_c15270 are separated by 212 nucleotides PGA1_c15270 and PGA1_c15280 are separated by 94 nucleotides PGA1_c15280 and PGA1_c15290 are separated by 80 nucleotides
PGA1_c15260: PGA1_c15260 - protein recA (recombinase A), at 1,589,307 to 1,590,374
_c15260
PGA1_c15270: PGA1_c15270 - alanyl-tRNA synthetase AlaS, at 1,590,587 to 1,593,238
_c15270
PGA1_c15280: PGA1_c15280 - Uncharacterized conserved protein, at 1,593,333 to 1,593,620
_c15280
PGA1_c15290: PGA1_c15290 - Lactoylglutathione lyase and related lyases, at 1,593,701 to 1,594,282
_c15290
Position (kb)
1590
1591
1592
1593
1594 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1589.693 kb on + strand, within PGA1_c15260 at 1589.943 kb on + strand, within PGA1_c15260 at 1590.068 kb on + strand, within PGA1_c15260 at 1590.234 kb on + strand, within PGA1_c15260 at 1590.234 kb on + strand, within PGA1_c15260 at 1590.308 kb on - strand at 1590.315 kb on + strand at 1590.397 kb on + strand at 1590.515 kb on + strand at 1590.515 kb on + strand at 1590.515 kb on + strand at 1590.518 kb on - strand at 1593.238 kb on + strand at 1593.314 kb on + strand at 1593.519 kb on - strand, within PGA1_c15280 at 1593.558 kb on + strand, within PGA1_c15280 at 1593.558 kb on + strand, within PGA1_c15280 at 1593.566 kb on - strand, within PGA1_c15280 at 1593.573 kb on - strand, within PGA1_c15280 at 1593.588 kb on - strand, within PGA1_c15280 at 1593.641 kb on + strand at 1593.649 kb on - strand at 1593.718 kb on - strand at 1593.718 kb on - strand at 1593.718 kb on - strand at 1593.732 kb on + strand at 1593.788 kb on + strand, within PGA1_c15290 at 1593.836 kb on + strand, within PGA1_c15290 at 1594.036 kb on - strand, within PGA1_c15290 at 1594.085 kb on + strand, within PGA1_c15290 at 1594.238 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 1,589,693 + PGA1_c15260 0.36 +1.6 1,589,943 + PGA1_c15260 0.60 -1.0 1,590,068 + PGA1_c15260 0.71 -2.2 1,590,234 + PGA1_c15260 0.87 -1.7 1,590,234 + PGA1_c15260 0.87 -0.5 1,590,308 - +2.8 1,590,315 + +0.0 1,590,397 + -0.7 1,590,515 + -2.2 1,590,515 + +0.1 1,590,515 + +1.9 1,590,518 - -0.5 1,593,238 + -0.2 1,593,314 + +1.1 1,593,519 - PGA1_c15280 0.65 +0.0 1,593,558 + PGA1_c15280 0.78 +0.6 1,593,558 + PGA1_c15280 0.78 +0.9 1,593,566 - PGA1_c15280 0.81 +2.0 1,593,573 - PGA1_c15280 0.83 +0.6 1,593,588 - PGA1_c15280 0.89 +1.9 1,593,641 + -1.2 1,593,649 - -0.2 1,593,718 - -1.4 1,593,718 - -0.6 1,593,718 - +1.3 1,593,732 + -0.5 1,593,788 + PGA1_c15290 0.15 +0.2 1,593,836 + PGA1_c15290 0.23 -0.3 1,594,036 - PGA1_c15290 0.58 +0.4 1,594,085 + PGA1_c15290 0.66 +0.4 1,594,238 + -1.0
Or see this region's nucleotide sequence