Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01180 and PGA1_c01190 are separated by 9 nucleotides PGA1_c01190 and PGA1_c01200 are separated by 0 nucleotides PGA1_c01200 and PGA1_c01210 are separated by 34 nucleotides PGA1_c01210 and PGA1_c01220 are separated by 92 nucleotides
PGA1_c01180: PGA1_c01180 - Predicted P-loop-containing kinase, at 111,462 to 112,352
_c01180
PGA1_c01190: PGA1_c01190 - putative PTS system fructose subfamily IIA component, at 112,362 to 112,769
_c01190
PGA1_c01200: PGA1_c01200 - putative phosphocarrier, HPr family, at 112,770 to 113,039
_c01200
PGA1_c01210: PGA1_c01210 - Putative hemolysin, at 113,074 to 113,964
_c01210
PGA1_c01220: PGA1_c01220 - putative 3-hydroxybutyryl-CoA dehydrogenase, at 114,057 to 114,932
_c01220
Position (kb)
113
114 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 112.084 kb on + strand, within PGA1_c01180 at 112.084 kb on + strand, within PGA1_c01180 at 112.084 kb on + strand, within PGA1_c01180 at 112.084 kb on + strand, within PGA1_c01180 at 112.084 kb on + strand, within PGA1_c01180 at 112.084 kb on + strand, within PGA1_c01180 at 112.084 kb on + strand, within PGA1_c01180 at 112.084 kb on + strand, within PGA1_c01180 at 112.084 kb on + strand, within PGA1_c01180 at 112.087 kb on - strand, within PGA1_c01180 at 112.092 kb on - strand, within PGA1_c01180 at 112.092 kb on - strand, within PGA1_c01180 at 112.092 kb on - strand, within PGA1_c01180 at 112.104 kb on + strand, within PGA1_c01180 at 112.112 kb on + strand, within PGA1_c01180 at 112.122 kb on + strand, within PGA1_c01180 at 112.242 kb on + strand, within PGA1_c01180 at 112.250 kb on - strand, within PGA1_c01180 at 112.255 kb on - strand, within PGA1_c01180 at 112.263 kb on - strand, within PGA1_c01180 at 112.300 kb on + strand at 112.308 kb on - strand at 112.312 kb on + strand at 112.781 kb on - strand at 112.835 kb on + strand, within PGA1_c01200 at 112.835 kb on + strand, within PGA1_c01200 at 112.872 kb on - strand, within PGA1_c01200 at 112.933 kb on + strand, within PGA1_c01200 at 112.988 kb on + strand, within PGA1_c01200 at 113.035 kb on + strand at 113.063 kb on + strand at 113.063 kb on + strand at 113.063 kb on + strand at 113.071 kb on - strand at 113.071 kb on - strand at 113.105 kb on - strand at 113.117 kb on + strand at 113.117 kb on + strand at 113.217 kb on + strand, within PGA1_c01210 at 113.265 kb on + strand, within PGA1_c01210 at 113.349 kb on + strand, within PGA1_c01210 at 113.357 kb on - strand, within PGA1_c01210 at 113.372 kb on + strand, within PGA1_c01210 at 113.441 kb on - strand, within PGA1_c01210 at 113.457 kb on - strand, within PGA1_c01210 at 113.474 kb on + strand, within PGA1_c01210 at 113.479 kb on + strand, within PGA1_c01210 at 113.482 kb on - strand, within PGA1_c01210 at 113.507 kb on - strand, within PGA1_c01210 at 113.571 kb on - strand, within PGA1_c01210 at 113.814 kb on + strand, within PGA1_c01210 at 113.912 kb on + strand at 113.950 kb on + strand at 114.027 kb on - strand at 114.027 kb on - strand at 114.242 kb on + strand, within PGA1_c01220 at 114.313 kb on + strand, within PGA1_c01220 at 114.313 kb on + strand, within PGA1_c01220 at 114.411 kb on - strand, within PGA1_c01220 at 114.433 kb on - strand, within PGA1_c01220 at 114.433 kb on - strand, within PGA1_c01220 at 114.436 kb on + strand, within PGA1_c01220 at 114.447 kb on + strand, within PGA1_c01220 at 114.452 kb on + strand, within PGA1_c01220 at 114.452 kb on + strand, within PGA1_c01220 at 114.466 kb on + strand, within PGA1_c01220 at 114.474 kb on - strand, within PGA1_c01220 at 114.520 kb on + strand, within PGA1_c01220 at 114.520 kb on + strand, within PGA1_c01220 at 114.530 kb on + strand, within PGA1_c01220 at 114.530 kb on + strand, within PGA1_c01220 at 114.538 kb on - strand, within PGA1_c01220 at 114.649 kb on - strand, within PGA1_c01220 at 114.649 kb on - strand, within PGA1_c01220 at 114.649 kb on - strand, within PGA1_c01220 at 114.649 kb on - strand, within PGA1_c01220 at 114.651 kb on + strand, within PGA1_c01220 at 114.704 kb on + strand, within PGA1_c01220 at 114.704 kb on + strand, within PGA1_c01220 at 114.712 kb on - strand, within PGA1_c01220 at 114.712 kb on - strand, within PGA1_c01220 at 114.723 kb on - strand, within PGA1_c01220 at 114.760 kb on - strand, within PGA1_c01220 at 114.779 kb on - strand, within PGA1_c01220 at 114.904 kb on - strand at 114.904 kb on - strand at 114.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 112,084 + PGA1_c01180 0.70 +0.1 112,084 + PGA1_c01180 0.70 -0.2 112,084 + PGA1_c01180 0.70 +0.8 112,084 + PGA1_c01180 0.70 -2.3 112,084 + PGA1_c01180 0.70 +0.8 112,084 + PGA1_c01180 0.70 +0.4 112,084 + PGA1_c01180 0.70 -0.8 112,084 + PGA1_c01180 0.70 +1.0 112,084 + PGA1_c01180 0.70 -2.2 112,087 - PGA1_c01180 0.70 +1.6 112,092 - PGA1_c01180 0.71 -0.7 112,092 - PGA1_c01180 0.71 +1.6 112,092 - PGA1_c01180 0.71 +0.6 112,104 + PGA1_c01180 0.72 +0.6 112,112 + PGA1_c01180 0.73 -0.9 112,122 + PGA1_c01180 0.74 -0.4 112,242 + PGA1_c01180 0.88 +0.2 112,250 - PGA1_c01180 0.88 +0.6 112,255 - PGA1_c01180 0.89 -0.7 112,263 - PGA1_c01180 0.90 -0.7 112,300 + +0.2 112,308 - -0.4 112,312 + +1.2 112,781 - -0.7 112,835 + PGA1_c01200 0.24 -0.1 112,835 + PGA1_c01200 0.24 -1.5 112,872 - PGA1_c01200 0.38 -0.6 112,933 + PGA1_c01200 0.60 -0.6 112,988 + PGA1_c01200 0.81 -0.4 113,035 + -0.4 113,063 + +1.3 113,063 + -0.4 113,063 + +0.2 113,071 - -1.4 113,071 - +0.1 113,105 - -0.5 113,117 + +0.9 113,117 + +0.9 113,217 + PGA1_c01210 0.16 +3.4 113,265 + PGA1_c01210 0.21 -0.9 113,349 + PGA1_c01210 0.31 +0.6 113,357 - PGA1_c01210 0.32 +0.6 113,372 + PGA1_c01210 0.33 +2.3 113,441 - PGA1_c01210 0.41 +0.4 113,457 - PGA1_c01210 0.43 +2.5 113,474 + PGA1_c01210 0.45 +2.0 113,479 + PGA1_c01210 0.45 -1.7 113,482 - PGA1_c01210 0.46 +1.0 113,507 - PGA1_c01210 0.49 +2.2 113,571 - PGA1_c01210 0.56 +1.1 113,814 + PGA1_c01210 0.83 +0.9 113,912 + +2.1 113,950 + +1.0 114,027 - -0.2 114,027 - +0.9 114,242 + PGA1_c01220 0.21 -0.7 114,313 + PGA1_c01220 0.29 +0.6 114,313 + PGA1_c01220 0.29 +0.2 114,411 - PGA1_c01220 0.40 +0.2 114,433 - PGA1_c01220 0.43 -2.4 114,433 - PGA1_c01220 0.43 -0.7 114,436 + PGA1_c01220 0.43 +0.3 114,447 + PGA1_c01220 0.45 -1.9 114,452 + PGA1_c01220 0.45 +1.6 114,452 + PGA1_c01220 0.45 +0.3 114,466 + PGA1_c01220 0.47 -0.6 114,474 - PGA1_c01220 0.48 -0.6 114,520 + PGA1_c01220 0.53 -0.9 114,520 + PGA1_c01220 0.53 +0.7 114,530 + PGA1_c01220 0.54 +0.1 114,530 + PGA1_c01220 0.54 -0.9 114,538 - PGA1_c01220 0.55 -0.4 114,649 - PGA1_c01220 0.68 -0.5 114,649 - PGA1_c01220 0.68 -2.2 114,649 - PGA1_c01220 0.68 -0.6 114,649 - PGA1_c01220 0.68 +0.3 114,651 + PGA1_c01220 0.68 -0.7 114,704 + PGA1_c01220 0.74 -1.1 114,704 + PGA1_c01220 0.74 -0.3 114,712 - PGA1_c01220 0.75 -0.7 114,712 - PGA1_c01220 0.75 -0.9 114,723 - PGA1_c01220 0.76 -0.7 114,760 - PGA1_c01220 0.80 +1.2 114,779 - PGA1_c01220 0.82 -1.2 114,904 - -0.4 114,904 - +0.1 114,935 - +0.2
Or see this region's nucleotide sequence