Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04350 and PGA1_c04360 overlap by 4 nucleotides PGA1_c04360 and PGA1_c04370 overlap by 4 nucleotides PGA1_c04370 and PGA1_c04380 are separated by 80 nucleotides
PGA1_c04350: PGA1_c04350 - putative glycosyltransferase protein, at 419,898 to 421,040
_c04350
PGA1_c04360: PGA1_c04360 - putative phosphoglycerate mutase family protein, at 421,037 to 421,627
_c04360
PGA1_c04370: PGA1_c04370 - putative glycosyltransferase protein, at 421,624 to 422,658
_c04370
PGA1_c04380: PGA1_c04380 - putative colanic acid biosynthesis glycosyltransferase WcaL, at 422,739 to 423,998
_c04380
Position (kb)
421
422
423 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 420.665 kb on + strand, within PGA1_c04350 at 420.665 kb on + strand, within PGA1_c04350 at 420.665 kb on + strand, within PGA1_c04350 at 420.665 kb on + strand, within PGA1_c04350 at 420.678 kb on + strand, within PGA1_c04350 at 420.701 kb on + strand, within PGA1_c04350 at 420.701 kb on + strand, within PGA1_c04350 at 420.701 kb on + strand, within PGA1_c04350 at 420.719 kb on - strand, within PGA1_c04350 at 420.746 kb on - strand, within PGA1_c04350 at 420.775 kb on - strand, within PGA1_c04350 at 420.775 kb on - strand, within PGA1_c04350 at 420.780 kb on - strand, within PGA1_c04350 at 420.780 kb on - strand, within PGA1_c04350 at 420.782 kb on + strand, within PGA1_c04350 at 420.804 kb on + strand, within PGA1_c04350 at 420.812 kb on - strand, within PGA1_c04350 at 420.895 kb on - strand, within PGA1_c04350 at 420.902 kb on + strand, within PGA1_c04350 at 420.910 kb on - strand, within PGA1_c04350 at 420.910 kb on - strand, within PGA1_c04350 at 420.910 kb on - strand, within PGA1_c04350 at 420.922 kb on - strand, within PGA1_c04350 at 420.940 kb on - strand at 420.977 kb on + strand at 420.990 kb on + strand at 420.993 kb on + strand at 420.998 kb on + strand at 420.998 kb on + strand at 421.003 kb on + strand at 421.144 kb on + strand, within PGA1_c04360 at 421.215 kb on + strand, within PGA1_c04360 at 421.243 kb on + strand, within PGA1_c04360 at 421.297 kb on - strand, within PGA1_c04360 at 421.313 kb on + strand, within PGA1_c04360 at 421.321 kb on - strand, within PGA1_c04360 at 421.355 kb on - strand, within PGA1_c04360 at 421.355 kb on - strand, within PGA1_c04360 at 421.362 kb on - strand, within PGA1_c04360 at 421.372 kb on + strand, within PGA1_c04360 at 421.377 kb on - strand, within PGA1_c04360 at 421.377 kb on - strand, within PGA1_c04360 at 421.380 kb on - strand, within PGA1_c04360 at 421.391 kb on - strand, within PGA1_c04360 at 421.468 kb on + strand, within PGA1_c04360 at 421.476 kb on - strand, within PGA1_c04360 at 421.480 kb on - strand, within PGA1_c04360 at 421.503 kb on + strand, within PGA1_c04360 at 421.534 kb on - strand, within PGA1_c04360 at 421.536 kb on + strand, within PGA1_c04360 at 421.591 kb on - strand at 421.604 kb on + strand at 421.645 kb on + strand at 421.690 kb on + strand at 421.726 kb on - strand at 421.748 kb on - strand, within PGA1_c04370 at 421.808 kb on - strand, within PGA1_c04370 at 421.819 kb on - strand, within PGA1_c04370 at 421.828 kb on + strand, within PGA1_c04370 at 421.838 kb on + strand, within PGA1_c04370 at 421.846 kb on - strand, within PGA1_c04370 at 421.848 kb on + strand, within PGA1_c04370 at 421.856 kb on - strand, within PGA1_c04370 at 421.936 kb on + strand, within PGA1_c04370 at 422.026 kb on + strand, within PGA1_c04370 at 422.039 kb on - strand, within PGA1_c04370 at 422.044 kb on - strand, within PGA1_c04370 at 422.050 kb on - strand, within PGA1_c04370 at 422.084 kb on + strand, within PGA1_c04370 at 422.141 kb on + strand, within PGA1_c04370 at 422.141 kb on + strand, within PGA1_c04370 at 422.146 kb on + strand, within PGA1_c04370 at 422.169 kb on - strand, within PGA1_c04370 at 422.171 kb on - strand, within PGA1_c04370 at 422.272 kb on + strand, within PGA1_c04370 at 422.280 kb on - strand, within PGA1_c04370 at 422.280 kb on - strand, within PGA1_c04370 at 422.302 kb on + strand, within PGA1_c04370 at 422.396 kb on - strand, within PGA1_c04370 at 422.537 kb on + strand, within PGA1_c04370 at 422.545 kb on - strand, within PGA1_c04370 at 422.619 kb on + strand at 422.647 kb on - strand at 422.647 kb on - strand at 422.669 kb on + strand at 422.675 kb on - strand at 422.682 kb on - strand at 422.712 kb on + strand at 422.735 kb on + strand at 422.743 kb on - strand at 422.828 kb on - strand at 422.852 kb on + strand at 422.878 kb on - strand, within PGA1_c04380 at 422.879 kb on - strand, within PGA1_c04380 at 422.892 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.918 kb on + strand, within PGA1_c04380 at 422.926 kb on - strand, within PGA1_c04380 at 422.926 kb on - strand, within PGA1_c04380 at 422.926 kb on - strand, within PGA1_c04380 at 422.943 kb on + strand, within PGA1_c04380 at 422.961 kb on - strand, within PGA1_c04380 at 422.962 kb on + strand, within PGA1_c04380 at 422.983 kb on + strand, within PGA1_c04380 at 423.041 kb on + strand, within PGA1_c04380 at 423.049 kb on - strand, within PGA1_c04380 at 423.065 kb on + strand, within PGA1_c04380 at 423.073 kb on - strand, within PGA1_c04380 at 423.073 kb on - strand, within PGA1_c04380 at 423.238 kb on + strand, within PGA1_c04380 at 423.293 kb on - strand, within PGA1_c04380 at 423.350 kb on + strand, within PGA1_c04380 at 423.350 kb on + strand, within PGA1_c04380 at 423.350 kb on + strand, within PGA1_c04380 at 423.350 kb on + strand, within PGA1_c04380 at 423.358 kb on - strand, within PGA1_c04380 at 423.394 kb on + strand, within PGA1_c04380 at 423.394 kb on + strand, within PGA1_c04380 at 423.394 kb on + strand, within PGA1_c04380 at 423.397 kb on - strand, within PGA1_c04380 at 423.451 kb on + strand, within PGA1_c04380 at 423.499 kb on + strand, within PGA1_c04380 at 423.499 kb on + strand, within PGA1_c04380 at 423.499 kb on + strand, within PGA1_c04380 at 423.529 kb on + strand, within PGA1_c04380 at 423.534 kb on + strand, within PGA1_c04380 at 423.537 kb on - strand, within PGA1_c04380 at 423.585 kb on - strand, within PGA1_c04380 at 423.585 kb on - strand, within PGA1_c04380 at 423.645 kb on - strand, within PGA1_c04380 at 423.645 kb on - strand, within PGA1_c04380 at 423.650 kb on - strand, within PGA1_c04380 at 423.650 kb on - strand, within PGA1_c04380 at 423.650 kb on - strand, within PGA1_c04380 at 423.650 kb on - strand, within PGA1_c04380
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 420,665 + PGA1_c04350 0.67 -2.5 420,665 + PGA1_c04350 0.67 -0.2 420,665 + PGA1_c04350 0.67 -1.6 420,665 + PGA1_c04350 0.67 -1.3 420,678 + PGA1_c04350 0.68 -0.5 420,701 + PGA1_c04350 0.70 -0.5 420,701 + PGA1_c04350 0.70 -0.1 420,701 + PGA1_c04350 0.70 +2.1 420,719 - PGA1_c04350 0.72 -0.6 420,746 - PGA1_c04350 0.74 +1.1 420,775 - PGA1_c04350 0.77 -0.1 420,775 - PGA1_c04350 0.77 -0.8 420,780 - PGA1_c04350 0.77 -0.6 420,780 - PGA1_c04350 0.77 -0.7 420,782 + PGA1_c04350 0.77 +0.2 420,804 + PGA1_c04350 0.79 -1.3 420,812 - PGA1_c04350 0.80 +0.3 420,895 - PGA1_c04350 0.87 -1.1 420,902 + PGA1_c04350 0.88 -2.4 420,910 - PGA1_c04350 0.89 -1.1 420,910 - PGA1_c04350 0.89 -2.2 420,910 - PGA1_c04350 0.89 -1.6 420,922 - PGA1_c04350 0.90 -1.1 420,940 - +2.5 420,977 + -0.1 420,990 + -0.6 420,993 + +0.8 420,998 + +0.7 420,998 + -1.8 421,003 + -0.1 421,144 + PGA1_c04360 0.18 +1.1 421,215 + PGA1_c04360 0.30 -1.8 421,243 + PGA1_c04360 0.35 -0.1 421,297 - PGA1_c04360 0.44 -0.8 421,313 + PGA1_c04360 0.47 -1.1 421,321 - PGA1_c04360 0.48 -1.5 421,355 - PGA1_c04360 0.54 -0.1 421,355 - PGA1_c04360 0.54 -0.1 421,362 - PGA1_c04360 0.55 -0.8 421,372 + PGA1_c04360 0.57 -1.8 421,377 - PGA1_c04360 0.58 -0.8 421,377 - PGA1_c04360 0.58 -0.5 421,380 - PGA1_c04360 0.58 -0.5 421,391 - PGA1_c04360 0.60 -0.8 421,468 + PGA1_c04360 0.73 -0.1 421,476 - PGA1_c04360 0.74 +1.8 421,480 - PGA1_c04360 0.75 -0.7 421,503 + PGA1_c04360 0.79 +0.8 421,534 - PGA1_c04360 0.84 +0.9 421,536 + PGA1_c04360 0.84 -0.1 421,591 - -2.1 421,604 + -0.2 421,645 + -0.9 421,690 + -0.7 421,726 - +0.2 421,748 - PGA1_c04370 0.12 +0.9 421,808 - PGA1_c04370 0.18 +0.5 421,819 - PGA1_c04370 0.19 +0.5 421,828 + PGA1_c04370 0.20 -2.4 421,838 + PGA1_c04370 0.21 -1.1 421,846 - PGA1_c04370 0.21 -0.5 421,848 + PGA1_c04370 0.22 -2.3 421,856 - PGA1_c04370 0.22 +0.4 421,936 + PGA1_c04370 0.30 +0.5 422,026 + PGA1_c04370 0.39 -2.1 422,039 - PGA1_c04370 0.40 -1.3 422,044 - PGA1_c04370 0.41 +0.2 422,050 - PGA1_c04370 0.41 -1.0 422,084 + PGA1_c04370 0.44 +0.5 422,141 + PGA1_c04370 0.50 +0.4 422,141 + PGA1_c04370 0.50 -1.3 422,146 + PGA1_c04370 0.50 -1.0 422,169 - PGA1_c04370 0.53 +1.0 422,171 - PGA1_c04370 0.53 -0.5 422,272 + PGA1_c04370 0.63 +1.1 422,280 - PGA1_c04370 0.63 -0.9 422,280 - PGA1_c04370 0.63 +1.5 422,302 + PGA1_c04370 0.66 -1.1 422,396 - PGA1_c04370 0.75 +0.3 422,537 + PGA1_c04370 0.88 -0.8 422,545 - PGA1_c04370 0.89 -1.2 422,619 + -1.5 422,647 - -0.5 422,647 - -0.2 422,669 + +0.4 422,675 - -0.3 422,682 - -1.2 422,712 + -0.3 422,735 + +1.9 422,743 - +0.4 422,828 - -1.2 422,852 + +1.5 422,878 - PGA1_c04380 0.11 -0.5 422,879 - PGA1_c04380 0.11 +0.5 422,892 + PGA1_c04380 0.12 -0.1 422,918 + PGA1_c04380 0.14 -1.5 422,918 + PGA1_c04380 0.14 -0.1 422,918 + PGA1_c04380 0.14 +1.9 422,918 + PGA1_c04380 0.14 +0.5 422,918 + PGA1_c04380 0.14 -1.2 422,918 + PGA1_c04380 0.14 +1.5 422,918 + PGA1_c04380 0.14 +1.5 422,918 + PGA1_c04380 0.14 -0.3 422,918 + PGA1_c04380 0.14 -0.3 422,926 - PGA1_c04380 0.15 -0.8 422,926 - PGA1_c04380 0.15 -0.1 422,926 - PGA1_c04380 0.15 -0.6 422,943 + PGA1_c04380 0.16 -0.8 422,961 - PGA1_c04380 0.18 -0.8 422,962 + PGA1_c04380 0.18 -1.2 422,983 + PGA1_c04380 0.19 +0.2 423,041 + PGA1_c04380 0.24 -0.4 423,049 - PGA1_c04380 0.25 +0.3 423,065 + PGA1_c04380 0.26 -0.3 423,073 - PGA1_c04380 0.27 -0.8 423,073 - PGA1_c04380 0.27 -0.3 423,238 + PGA1_c04380 0.40 -0.4 423,293 - PGA1_c04380 0.44 +0.5 423,350 + PGA1_c04380 0.48 -0.8 423,350 + PGA1_c04380 0.48 -1.6 423,350 + PGA1_c04380 0.48 -0.4 423,350 + PGA1_c04380 0.48 -0.8 423,358 - PGA1_c04380 0.49 -0.8 423,394 + PGA1_c04380 0.52 -0.5 423,394 + PGA1_c04380 0.52 -0.5 423,394 + PGA1_c04380 0.52 -0.1 423,397 - PGA1_c04380 0.52 +0.8 423,451 + PGA1_c04380 0.57 -1.1 423,499 + PGA1_c04380 0.60 +0.7 423,499 + PGA1_c04380 0.60 +0.4 423,499 + PGA1_c04380 0.60 -0.5 423,529 + PGA1_c04380 0.63 +0.5 423,534 + PGA1_c04380 0.63 -0.5 423,537 - PGA1_c04380 0.63 -1.1 423,585 - PGA1_c04380 0.67 -1.4 423,585 - PGA1_c04380 0.67 +0.2 423,645 - PGA1_c04380 0.72 +0.5 423,645 - PGA1_c04380 0.72 -2.1 423,650 - PGA1_c04380 0.72 -0.1 423,650 - PGA1_c04380 0.72 +0.9 423,650 - PGA1_c04380 0.72 -1.2 423,650 - PGA1_c04380 0.72 -0.2
Or see this region's nucleotide sequence