Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01460 and PGA1_262p01470 are separated by 65 nucleotides PGA1_262p01470 and PGA1_262p01480 overlap by 4 nucleotides PGA1_262p01480 and PGA1_262p01490 are separated by 32 nucleotides PGA1_262p01490 and PGA1_262p01500 overlap by 8 nucleotides
PGA1_262p01460: PGA1_262p01460 - aldehyde dehydrogenase, at 159,077 to 160,570
_262p01460
PGA1_262p01470: PGA1_262p01470 - transcriptional regulator, LysR family, at 160,636 to 161,532
_262p01470
PGA1_262p01480: PGA1_262p01480 - transcriptional regulator, AraC family, at 161,529 to 162,536
_262p01480
PGA1_262p01490: PGA1_262p01490 - putative sarcosine oxidase, gamma subunit, at 162,569 to 163,132
_262p01490
PGA1_262p01500: PGA1_262p01500 - sarcosine oxidase subunit alpha, at 163,125 to 166,091
_262p01500
Position (kb) 161
162
163 Strain fitness (log2 ratio) -1
0
1
2 at 160.534 kb on - strand at 160.569 kb on - strand at 160.929 kb on + strand, within PGA1_262p01470 at 160.949 kb on + strand, within PGA1_262p01470 at 161.027 kb on + strand, within PGA1_262p01470 at 161.091 kb on - strand, within PGA1_262p01470 at 161.129 kb on + strand, within PGA1_262p01470 at 161.129 kb on + strand, within PGA1_262p01470 at 161.251 kb on - strand, within PGA1_262p01470 at 161.268 kb on + strand, within PGA1_262p01470 at 161.276 kb on - strand, within PGA1_262p01470 at 161.276 kb on - strand, within PGA1_262p01470 at 161.300 kb on + strand, within PGA1_262p01470 at 161.457 kb on + strand at 161.457 kb on + strand at 161.457 kb on + strand at 161.457 kb on + strand at 161.457 kb on + strand at 161.534 kb on - strand at 161.591 kb on - strand at 161.613 kb on - strand at 162.040 kb on + strand, within PGA1_262p01480 at 162.267 kb on - strand, within PGA1_262p01480 at 162.312 kb on - strand, within PGA1_262p01480 at 162.317 kb on - strand, within PGA1_262p01480 at 162.588 kb on + strand at 162.588 kb on + strand at 162.694 kb on - strand, within PGA1_262p01490 at 162.740 kb on + strand, within PGA1_262p01490 at 162.917 kb on - strand, within PGA1_262p01490 at 162.917 kb on - strand, within PGA1_262p01490 at 163.116 kb on - strand at 163.136 kb on + strand at 163.246 kb on + strand at 163.302 kb on + strand at 163.310 kb on - strand at 163.436 kb on - strand, within PGA1_262p01500
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 160,534 - +0.9 160,569 - -0.8 160,929 + PGA1_262p01470 0.33 -0.1 160,949 + PGA1_262p01470 0.35 +1.2 161,027 + PGA1_262p01470 0.44 +0.3 161,091 - PGA1_262p01470 0.51 +0.7 161,129 + PGA1_262p01470 0.55 +0.2 161,129 + PGA1_262p01470 0.55 -0.7 161,251 - PGA1_262p01470 0.69 -1.7 161,268 + PGA1_262p01470 0.70 +1.7 161,276 - PGA1_262p01470 0.71 -1.0 161,276 - PGA1_262p01470 0.71 -0.4 161,300 + PGA1_262p01470 0.74 -0.1 161,457 + -0.5 161,457 + +0.6 161,457 + -0.2 161,457 + +1.0 161,457 + +0.3 161,534 - +0.4 161,591 - +0.6 161,613 - -0.7 162,040 + PGA1_262p01480 0.51 -0.4 162,267 - PGA1_262p01480 0.73 -0.3 162,312 - PGA1_262p01480 0.78 -1.0 162,317 - PGA1_262p01480 0.78 -1.4 162,588 + +0.8 162,588 + +2.4 162,694 - PGA1_262p01490 0.22 -1.2 162,740 + PGA1_262p01490 0.30 +0.9 162,917 - PGA1_262p01490 0.62 +0.6 162,917 - PGA1_262p01490 0.62 -0.1 163,116 - +0.9 163,136 + +1.2 163,246 + +0.8 163,302 + -0.4 163,310 - +0.4 163,436 - PGA1_262p01500 0.10 +0.5
Or see this region's nucleotide sequence