Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c32690 and PGA1_c32700 are separated by 31 nucleotides PGA1_c32700 and PGA1_c32710 are separated by 118 nucleotides PGA1_c32710 and PGA1_c32720 are separated by 99 nucleotides
PGA1_c32690: PGA1_c32690 - putative saccharopine dehydrogenase (NAD+, L-lysine-forming), at 3,426,520 to 3,427,572
_c32690
PGA1_c32700: PGA1_c32700 - putative saccharopine dehydrogenase (NADP+, L-glutamate-forming), at 3,427,604 to 3,428,746
_c32700
PGA1_c32710: PGA1_c32710 - Protein of unknown function (DUF3775)., at 3,428,865 to 3,429,188
_c32710
PGA1_c32720: PGA1_c32720 - putative adenylate and guanylate cyclase, at 3,429,288 to 3,430,589
_c32720
Position (kb)
3427
3428
3429 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3426.610 kb on + strand at 3426.635 kb on + strand, within PGA1_c32690 at 3426.661 kb on + strand, within PGA1_c32690 at 3426.669 kb on - strand, within PGA1_c32690 at 3426.713 kb on + strand, within PGA1_c32690 at 3426.713 kb on + strand, within PGA1_c32690 at 3426.794 kb on + strand, within PGA1_c32690 at 3426.816 kb on - strand, within PGA1_c32690 at 3426.840 kb on + strand, within PGA1_c32690 at 3426.876 kb on - strand, within PGA1_c32690 at 3426.881 kb on - strand, within PGA1_c32690 at 3426.894 kb on - strand, within PGA1_c32690 at 3426.894 kb on - strand, within PGA1_c32690 at 3426.928 kb on + strand, within PGA1_c32690 at 3426.936 kb on - strand, within PGA1_c32690 at 3426.957 kb on + strand, within PGA1_c32690 at 3426.965 kb on - strand, within PGA1_c32690 at 3426.965 kb on - strand, within PGA1_c32690 at 3426.965 kb on - strand, within PGA1_c32690 at 3426.965 kb on - strand, within PGA1_c32690 at 3427.084 kb on - strand, within PGA1_c32690 at 3427.128 kb on - strand, within PGA1_c32690 at 3427.242 kb on + strand, within PGA1_c32690 at 3427.250 kb on + strand, within PGA1_c32690 at 3427.369 kb on + strand, within PGA1_c32690 at 3427.369 kb on + strand, within PGA1_c32690 at 3427.379 kb on + strand, within PGA1_c32690 at 3427.379 kb on + strand, within PGA1_c32690 at 3427.387 kb on - strand, within PGA1_c32690 at 3427.447 kb on - strand, within PGA1_c32690 at 3427.490 kb on + strand at 3427.491 kb on - strand at 3427.500 kb on + strand at 3427.586 kb on + strand at 3427.617 kb on + strand at 3427.631 kb on - strand at 3427.631 kb on - strand at 3427.631 kb on - strand at 3427.649 kb on - strand at 3427.693 kb on - strand at 3427.749 kb on + strand, within PGA1_c32700 at 3427.757 kb on - strand, within PGA1_c32700 at 3427.757 kb on - strand, within PGA1_c32700 at 3427.757 kb on - strand, within PGA1_c32700 at 3427.757 kb on - strand, within PGA1_c32700 at 3427.757 kb on - strand, within PGA1_c32700 at 3427.807 kb on - strand, within PGA1_c32700 at 3427.870 kb on - strand, within PGA1_c32700 at 3427.870 kb on - strand, within PGA1_c32700 at 3427.884 kb on - strand, within PGA1_c32700 at 3427.896 kb on + strand, within PGA1_c32700 at 3427.902 kb on + strand, within PGA1_c32700 at 3428.010 kb on + strand, within PGA1_c32700 at 3428.010 kb on + strand, within PGA1_c32700 at 3428.010 kb on + strand, within PGA1_c32700 at 3428.010 kb on + strand, within PGA1_c32700 at 3428.018 kb on - strand, within PGA1_c32700 at 3428.018 kb on - strand, within PGA1_c32700 at 3428.018 kb on - strand, within PGA1_c32700 at 3428.056 kb on - strand, within PGA1_c32700 at 3428.076 kb on - strand, within PGA1_c32700 at 3428.076 kb on - strand, within PGA1_c32700 at 3428.176 kb on - strand, within PGA1_c32700 at 3428.176 kb on - strand, within PGA1_c32700 at 3428.179 kb on + strand, within PGA1_c32700 at 3428.187 kb on - strand, within PGA1_c32700 at 3428.224 kb on - strand, within PGA1_c32700 at 3428.229 kb on - strand, within PGA1_c32700 at 3428.229 kb on - strand, within PGA1_c32700 at 3428.229 kb on - strand, within PGA1_c32700 at 3428.271 kb on + strand, within PGA1_c32700 at 3428.298 kb on + strand, within PGA1_c32700 at 3428.314 kb on - strand, within PGA1_c32700 at 3428.314 kb on - strand, within PGA1_c32700 at 3428.348 kb on + strand, within PGA1_c32700 at 3428.354 kb on - strand, within PGA1_c32700 at 3428.368 kb on + strand, within PGA1_c32700 at 3428.385 kb on + strand, within PGA1_c32700 at 3428.410 kb on - strand, within PGA1_c32700 at 3428.502 kb on - strand, within PGA1_c32700 at 3428.556 kb on - strand, within PGA1_c32700 at 3428.573 kb on + strand, within PGA1_c32700 at 3428.605 kb on - strand, within PGA1_c32700 at 3428.652 kb on + strand at 3428.711 kb on + strand at 3428.711 kb on + strand at 3428.719 kb on + strand at 3428.734 kb on - strand at 3428.758 kb on + strand at 3428.766 kb on - strand at 3428.766 kb on - strand at 3428.777 kb on - strand at 3428.777 kb on - strand at 3428.777 kb on - strand at 3428.811 kb on + strand at 3428.844 kb on - strand at 3428.885 kb on + strand at 3428.893 kb on - strand at 3428.900 kb on + strand, within PGA1_c32710 at 3429.037 kb on - strand, within PGA1_c32710 at 3429.119 kb on - strand, within PGA1_c32710 at 3429.144 kb on + strand, within PGA1_c32710 at 3429.152 kb on - strand, within PGA1_c32710 at 3429.232 kb on - strand at 3429.250 kb on + strand at 3429.294 kb on - strand at 3429.343 kb on - strand at 3429.381 kb on - strand at 3429.390 kb on + strand at 3429.409 kb on + strand at 3429.438 kb on + strand, within PGA1_c32720 at 3429.462 kb on + strand, within PGA1_c32720 at 3429.470 kb on - strand, within PGA1_c32720 at 3429.492 kb on - strand, within PGA1_c32720 at 3429.582 kb on - strand, within PGA1_c32720 at 3429.625 kb on + strand, within PGA1_c32720 at 3429.625 kb on + strand, within PGA1_c32720 at 3429.630 kb on + strand, within PGA1_c32720 at 3429.630 kb on + strand, within PGA1_c32720 at 3429.633 kb on - strand, within PGA1_c32720 at 3429.673 kb on - strand, within PGA1_c32720 at 3429.674 kb on + strand, within PGA1_c32720
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 3,426,610 + -0.5 3,426,635 + PGA1_c32690 0.11 -0.2 3,426,661 + PGA1_c32690 0.13 +0.5 3,426,669 - PGA1_c32690 0.14 -0.1 3,426,713 + PGA1_c32690 0.18 -0.9 3,426,713 + PGA1_c32690 0.18 -0.8 3,426,794 + PGA1_c32690 0.26 -0.8 3,426,816 - PGA1_c32690 0.28 +1.5 3,426,840 + PGA1_c32690 0.30 +0.5 3,426,876 - PGA1_c32690 0.34 -0.7 3,426,881 - PGA1_c32690 0.34 -1.0 3,426,894 - PGA1_c32690 0.36 +0.1 3,426,894 - PGA1_c32690 0.36 -0.2 3,426,928 + PGA1_c32690 0.39 +0.5 3,426,936 - PGA1_c32690 0.40 -0.8 3,426,957 + PGA1_c32690 0.42 +2.2 3,426,965 - PGA1_c32690 0.42 +0.3 3,426,965 - PGA1_c32690 0.42 +2.3 3,426,965 - PGA1_c32690 0.42 +0.4 3,426,965 - PGA1_c32690 0.42 -1.1 3,427,084 - PGA1_c32690 0.54 -0.5 3,427,128 - PGA1_c32690 0.58 +0.7 3,427,242 + PGA1_c32690 0.69 +0.9 3,427,250 + PGA1_c32690 0.69 -0.4 3,427,369 + PGA1_c32690 0.81 -0.1 3,427,369 + PGA1_c32690 0.81 -1.5 3,427,379 + PGA1_c32690 0.82 +0.5 3,427,379 + PGA1_c32690 0.82 -1.3 3,427,387 - PGA1_c32690 0.82 -0.1 3,427,447 - PGA1_c32690 0.88 +0.8 3,427,490 + -1.1 3,427,491 - +2.0 3,427,500 + +0.4 3,427,586 + +0.2 3,427,617 + -2.1 3,427,631 - -0.8 3,427,631 - -0.1 3,427,631 - -1.3 3,427,649 - -0.8 3,427,693 - -1.1 3,427,749 + PGA1_c32700 0.13 -0.1 3,427,757 - PGA1_c32700 0.13 +0.7 3,427,757 - PGA1_c32700 0.13 +2.5 3,427,757 - PGA1_c32700 0.13 -0.1 3,427,757 - PGA1_c32700 0.13 +0.8 3,427,757 - PGA1_c32700 0.13 +1.7 3,427,807 - PGA1_c32700 0.18 +0.9 3,427,870 - PGA1_c32700 0.23 +0.1 3,427,870 - PGA1_c32700 0.23 +1.9 3,427,884 - PGA1_c32700 0.24 +1.1 3,427,896 + PGA1_c32700 0.26 +1.3 3,427,902 + PGA1_c32700 0.26 -0.1 3,428,010 + PGA1_c32700 0.36 -1.1 3,428,010 + PGA1_c32700 0.36 -0.4 3,428,010 + PGA1_c32700 0.36 +0.5 3,428,010 + PGA1_c32700 0.36 -0.2 3,428,018 - PGA1_c32700 0.36 +1.3 3,428,018 - PGA1_c32700 0.36 -1.1 3,428,018 - PGA1_c32700 0.36 -2.9 3,428,056 - PGA1_c32700 0.40 -0.2 3,428,076 - PGA1_c32700 0.41 +0.5 3,428,076 - PGA1_c32700 0.41 +1.5 3,428,176 - PGA1_c32700 0.50 +0.5 3,428,176 - PGA1_c32700 0.50 -0.5 3,428,179 + PGA1_c32700 0.50 +0.1 3,428,187 - PGA1_c32700 0.51 -0.5 3,428,224 - PGA1_c32700 0.54 -0.1 3,428,229 - PGA1_c32700 0.55 -0.5 3,428,229 - PGA1_c32700 0.55 -1.3 3,428,229 - PGA1_c32700 0.55 +0.9 3,428,271 + PGA1_c32700 0.58 +0.3 3,428,298 + PGA1_c32700 0.61 +0.7 3,428,314 - PGA1_c32700 0.62 -0.6 3,428,314 - PGA1_c32700 0.62 -1.1 3,428,348 + PGA1_c32700 0.65 +0.2 3,428,354 - PGA1_c32700 0.66 +0.1 3,428,368 + PGA1_c32700 0.67 -0.6 3,428,385 + PGA1_c32700 0.68 -1.1 3,428,410 - PGA1_c32700 0.71 +1.1 3,428,502 - PGA1_c32700 0.79 +0.7 3,428,556 - PGA1_c32700 0.83 +2.4 3,428,573 + PGA1_c32700 0.85 -2.1 3,428,605 - PGA1_c32700 0.88 +0.6 3,428,652 + -1.1 3,428,711 + +0.6 3,428,711 + +0.2 3,428,719 + -0.5 3,428,734 - -1.0 3,428,758 + -1.0 3,428,766 - -1.3 3,428,766 - +0.8 3,428,777 - +1.8 3,428,777 - -1.3 3,428,777 - -0.5 3,428,811 + +0.5 3,428,844 - +0.2 3,428,885 + -0.5 3,428,893 - -0.1 3,428,900 + PGA1_c32710 0.11 +0.8 3,429,037 - PGA1_c32710 0.53 -0.1 3,429,119 - PGA1_c32710 0.78 -0.2 3,429,144 + PGA1_c32710 0.86 +0.7 3,429,152 - PGA1_c32710 0.89 -1.1 3,429,232 - -0.2 3,429,250 + +0.5 3,429,294 - -0.5 3,429,343 - -0.1 3,429,381 - -0.8 3,429,390 + +1.3 3,429,409 + -0.8 3,429,438 + PGA1_c32720 0.12 -1.3 3,429,462 + PGA1_c32720 0.13 +0.5 3,429,470 - PGA1_c32720 0.14 -1.3 3,429,492 - PGA1_c32720 0.16 -0.5 3,429,582 - PGA1_c32720 0.23 -0.2 3,429,625 + PGA1_c32720 0.26 -0.8 3,429,625 + PGA1_c32720 0.26 +0.5 3,429,630 + PGA1_c32720 0.26 -1.4 3,429,630 + PGA1_c32720 0.26 -1.5 3,429,633 - PGA1_c32720 0.26 +0.1 3,429,673 - PGA1_c32720 0.30 +1.3 3,429,674 + PGA1_c32720 0.30 +0.0
Or see this region's nucleotide sequence