Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22850 and PGA1_c22860 overlap by 8 nucleotides PGA1_c22860 and PGA1_c22870 overlap by 8 nucleotides PGA1_c22870 and PGA1_c22880 overlap by 4 nucleotides PGA1_c22880 and PGA1_c22890 are separated by 5 nucleotides
PGA1_c22850: PGA1_c22850 - type IV secretion system protein VirB, at 2,367,307 to 2,369,673
_c22850
PGA1_c22860: PGA1_c22860 - hypothetical protein, at 2,369,666 to 2,369,824
_c22860
PGA1_c22870: PGA1_c22870 - lytic transglycosylase-like protein, at 2,369,817 to 2,370,965
_c22870
PGA1_c22880: PGA1_c22880 - Type IV secretion system proteins., at 2,370,962 to 2,371,741
_c22880
PGA1_c22890: PGA1_c22890 - putative type IV secretion system protein VirB8, at 2,371,747 to 2,372,397
_c22890
Position (kb)
2369
2370
2371 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2368.840 kb on - strand, within PGA1_c22850 at 2368.919 kb on + strand, within PGA1_c22850 at 2368.963 kb on - strand, within PGA1_c22850 at 2368.972 kb on - strand, within PGA1_c22850 at 2369.012 kb on + strand, within PGA1_c22850 at 2369.037 kb on + strand, within PGA1_c22850 at 2369.045 kb on - strand, within PGA1_c22850 at 2369.062 kb on + strand, within PGA1_c22850 at 2369.181 kb on - strand, within PGA1_c22850 at 2369.217 kb on + strand, within PGA1_c22850 at 2369.222 kb on + strand, within PGA1_c22850 at 2369.225 kb on - strand, within PGA1_c22850 at 2369.225 kb on - strand, within PGA1_c22850 at 2369.225 kb on - strand, within PGA1_c22850 at 2369.257 kb on - strand, within PGA1_c22850 at 2369.306 kb on - strand, within PGA1_c22850 at 2369.452 kb on + strand at 2369.487 kb on + strand at 2369.535 kb on + strand at 2369.542 kb on + strand at 2369.579 kb on + strand at 2369.582 kb on + strand at 2369.661 kb on + strand at 2369.696 kb on - strand, within PGA1_c22860 at 2369.709 kb on - strand, within PGA1_c22860 at 2369.787 kb on - strand, within PGA1_c22860 at 2369.802 kb on - strand, within PGA1_c22860 at 2369.840 kb on - strand at 2369.847 kb on + strand at 2369.847 kb on + strand at 2369.865 kb on - strand at 2369.880 kb on + strand at 2369.928 kb on + strand at 2369.936 kb on + strand, within PGA1_c22870 at 2369.992 kb on + strand, within PGA1_c22870 at 2370.000 kb on - strand, within PGA1_c22870 at 2370.007 kb on + strand, within PGA1_c22870 at 2370.170 kb on - strand, within PGA1_c22870 at 2370.170 kb on - strand, within PGA1_c22870 at 2370.170 kb on - strand, within PGA1_c22870 at 2370.356 kb on + strand, within PGA1_c22870 at 2370.362 kb on + strand, within PGA1_c22870 at 2370.373 kb on + strand, within PGA1_c22870 at 2370.376 kb on - strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.378 kb on + strand, within PGA1_c22870 at 2370.386 kb on - strand, within PGA1_c22870 at 2370.386 kb on - strand, within PGA1_c22870 at 2370.525 kb on + strand, within PGA1_c22870 at 2370.525 kb on + strand, within PGA1_c22870 at 2370.571 kb on + strand, within PGA1_c22870 at 2370.571 kb on + strand, within PGA1_c22870 at 2370.571 kb on + strand, within PGA1_c22870 at 2370.579 kb on - strand, within PGA1_c22870 at 2370.579 kb on - strand, within PGA1_c22870 at 2370.579 kb on - strand, within PGA1_c22870 at 2370.579 kb on - strand, within PGA1_c22870 at 2370.581 kb on - strand, within PGA1_c22870 at 2370.675 kb on + strand, within PGA1_c22870 at 2370.685 kb on + strand, within PGA1_c22870 at 2370.693 kb on - strand, within PGA1_c22870 at 2370.701 kb on - strand, within PGA1_c22870 at 2370.765 kb on - strand, within PGA1_c22870 at 2370.775 kb on + strand, within PGA1_c22870 at 2370.857 kb on + strand at 2370.857 kb on + strand at 2370.857 kb on + strand at 2370.857 kb on + strand at 2370.865 kb on - strand at 2370.865 kb on - strand at 2370.865 kb on - strand at 2370.865 kb on - strand at 2370.865 kb on - strand at 2370.889 kb on + strand at 2370.897 kb on - strand at 2370.917 kb on + strand at 2370.991 kb on - strand at 2370.991 kb on - strand at 2371.008 kb on - strand at 2371.021 kb on - strand at 2371.022 kb on + strand at 2371.030 kb on - strand at 2371.107 kb on + strand, within PGA1_c22880 at 2371.117 kb on + strand, within PGA1_c22880 at 2371.125 kb on - strand, within PGA1_c22880 at 2371.177 kb on + strand, within PGA1_c22880 at 2371.179 kb on - strand, within PGA1_c22880 at 2371.182 kb on + strand, within PGA1_c22880 at 2371.182 kb on + strand, within PGA1_c22880 at 2371.185 kb on - strand, within PGA1_c22880 at 2371.185 kb on - strand, within PGA1_c22880 at 2371.187 kb on + strand, within PGA1_c22880 at 2371.187 kb on + strand, within PGA1_c22880 at 2371.187 kb on + strand, within PGA1_c22880 at 2371.187 kb on + strand, within PGA1_c22880 at 2371.195 kb on - strand, within PGA1_c22880 at 2371.195 kb on - strand, within PGA1_c22880 at 2371.207 kb on + strand, within PGA1_c22880 at 2371.227 kb on + strand, within PGA1_c22880 at 2371.278 kb on - strand, within PGA1_c22880 at 2371.358 kb on + strand, within PGA1_c22880 at 2371.368 kb on + strand, within PGA1_c22880 at 2371.493 kb on - strand, within PGA1_c22880 at 2371.523 kb on - strand, within PGA1_c22880 at 2371.650 kb on + strand, within PGA1_c22880 at 2371.663 kb on - strand, within PGA1_c22880 at 2371.720 kb on + strand at 2371.744 kb on + strand at 2371.744 kb on + strand at 2371.760 kb on - strand at 2371.790 kb on - strand at 2371.790 kb on - strand at 2371.790 kb on - strand at 2371.879 kb on + strand, within PGA1_c22890 at 2371.941 kb on - strand, within PGA1_c22890 at 2371.944 kb on + strand, within PGA1_c22890
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 2,368,840 - PGA1_c22850 0.65 -1.2 2,368,919 + PGA1_c22850 0.68 -1.6 2,368,963 - PGA1_c22850 0.70 -1.5 2,368,972 - PGA1_c22850 0.70 -2.4 2,369,012 + PGA1_c22850 0.72 +0.5 2,369,037 + PGA1_c22850 0.73 -1.3 2,369,045 - PGA1_c22850 0.73 -1.8 2,369,062 + PGA1_c22850 0.74 +1.2 2,369,181 - PGA1_c22850 0.79 -0.4 2,369,217 + PGA1_c22850 0.81 +0.6 2,369,222 + PGA1_c22850 0.81 -0.4 2,369,225 - PGA1_c22850 0.81 -0.8 2,369,225 - PGA1_c22850 0.81 -0.7 2,369,225 - PGA1_c22850 0.81 +0.7 2,369,257 - PGA1_c22850 0.82 -0.5 2,369,306 - PGA1_c22850 0.84 -0.2 2,369,452 + -0.2 2,369,487 + +0.2 2,369,535 + -0.1 2,369,542 + +2.1 2,369,579 + -2.5 2,369,582 + -1.8 2,369,661 + +0.1 2,369,696 - PGA1_c22860 0.19 -0.4 2,369,709 - PGA1_c22860 0.27 +1.2 2,369,787 - PGA1_c22860 0.76 +0.2 2,369,802 - PGA1_c22860 0.86 -0.2 2,369,840 - -0.8 2,369,847 + -1.1 2,369,847 + -0.6 2,369,865 - -0.0 2,369,880 + +2.4 2,369,928 + -1.1 2,369,936 + PGA1_c22870 0.10 -0.2 2,369,992 + PGA1_c22870 0.15 -0.2 2,370,000 - PGA1_c22870 0.16 +1.1 2,370,007 + PGA1_c22870 0.17 +0.2 2,370,170 - PGA1_c22870 0.31 +0.8 2,370,170 - PGA1_c22870 0.31 -1.5 2,370,170 - PGA1_c22870 0.31 -0.4 2,370,356 + PGA1_c22870 0.47 -0.6 2,370,362 + PGA1_c22870 0.47 -0.5 2,370,373 + PGA1_c22870 0.48 +0.4 2,370,376 - PGA1_c22870 0.49 -0.6 2,370,378 + PGA1_c22870 0.49 -0.7 2,370,378 + PGA1_c22870 0.49 +0.2 2,370,378 + PGA1_c22870 0.49 +0.1 2,370,378 + PGA1_c22870 0.49 -0.2 2,370,378 + PGA1_c22870 0.49 -0.6 2,370,378 + PGA1_c22870 0.49 +2.4 2,370,378 + PGA1_c22870 0.49 +0.7 2,370,378 + PGA1_c22870 0.49 +2.0 2,370,378 + PGA1_c22870 0.49 -0.5 2,370,386 - PGA1_c22870 0.50 +1.4 2,370,386 - PGA1_c22870 0.50 -2.1 2,370,525 + PGA1_c22870 0.62 +0.4 2,370,525 + PGA1_c22870 0.62 -0.6 2,370,571 + PGA1_c22870 0.66 -1.9 2,370,571 + PGA1_c22870 0.66 +2.4 2,370,571 + PGA1_c22870 0.66 -0.2 2,370,579 - PGA1_c22870 0.66 -0.2 2,370,579 - PGA1_c22870 0.66 -1.0 2,370,579 - PGA1_c22870 0.66 -0.8 2,370,579 - PGA1_c22870 0.66 -0.5 2,370,581 - PGA1_c22870 0.66 +0.1 2,370,675 + PGA1_c22870 0.75 -0.9 2,370,685 + PGA1_c22870 0.76 -1.1 2,370,693 - PGA1_c22870 0.76 -0.6 2,370,701 - PGA1_c22870 0.77 +0.0 2,370,765 - PGA1_c22870 0.83 -0.3 2,370,775 + PGA1_c22870 0.83 -0.7 2,370,857 + +0.4 2,370,857 + +0.1 2,370,857 + +1.4 2,370,857 + +0.3 2,370,865 - -1.9 2,370,865 - +0.2 2,370,865 - +0.7 2,370,865 - -0.4 2,370,865 - +0.8 2,370,889 + +1.4 2,370,897 - +0.2 2,370,917 + -0.5 2,370,991 - +1.4 2,370,991 - -1.3 2,371,008 - -0.2 2,371,021 - -1.8 2,371,022 + -0.4 2,371,030 - +0.7 2,371,107 + PGA1_c22880 0.19 +0.5 2,371,117 + PGA1_c22880 0.20 -1.6 2,371,125 - PGA1_c22880 0.21 +0.4 2,371,177 + PGA1_c22880 0.28 +0.1 2,371,179 - PGA1_c22880 0.28 +1.8 2,371,182 + PGA1_c22880 0.28 -0.2 2,371,182 + PGA1_c22880 0.28 +0.7 2,371,185 - PGA1_c22880 0.29 +0.8 2,371,185 - PGA1_c22880 0.29 +2.1 2,371,187 + PGA1_c22880 0.29 -0.3 2,371,187 + PGA1_c22880 0.29 -1.8 2,371,187 + PGA1_c22880 0.29 -0.5 2,371,187 + PGA1_c22880 0.29 -0.9 2,371,195 - PGA1_c22880 0.30 +1.4 2,371,195 - PGA1_c22880 0.30 -0.0 2,371,207 + PGA1_c22880 0.31 -1.0 2,371,227 + PGA1_c22880 0.34 +0.8 2,371,278 - PGA1_c22880 0.41 +1.0 2,371,358 + PGA1_c22880 0.51 -0.8 2,371,368 + PGA1_c22880 0.52 +0.6 2,371,493 - PGA1_c22880 0.68 -1.2 2,371,523 - PGA1_c22880 0.72 +0.1 2,371,650 + PGA1_c22880 0.88 -0.2 2,371,663 - PGA1_c22880 0.90 -0.5 2,371,720 + -0.3 2,371,744 + -0.2 2,371,744 + +0.3 2,371,760 - -0.9 2,371,790 - -0.6 2,371,790 - +0.4 2,371,790 - +1.4 2,371,879 + PGA1_c22890 0.20 -2.8 2,371,941 - PGA1_c22890 0.30 +1.0 2,371,944 + PGA1_c22890 0.30 +0.8
Or see this region's nucleotide sequence