Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c16820 and PGA1_c16830 are separated by 50 nucleotides PGA1_c16830 and PGA1_c16840 are separated by 205 nucleotides PGA1_c16840 and PGA1_c16850 are separated by 147 nucleotides
PGA1_c16820: PGA1_c16820 - putative threonine efflux protein, at 1,754,957 to 1,755,571
_c16820
PGA1_c16830: PGA1_c16830 - xanthine phosphoribosyltransferase Gpt, at 1,755,622 to 1,756,125
_c16830
PGA1_c16840: PGA1_c16840 - putative aspartate aminotransferase, at 1,756,331 to 1,757,521
_c16840
PGA1_c16850: PGA1_c16850 - putative peptidyl-prolyl cis-trans isomerase D, at 1,757,669 to 1,759,510
_c16850
Position (kb)
1756
1757
1758 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1755.335 kb on - strand, within PGA1_c16820 at 1755.336 kb on - strand, within PGA1_c16820 at 1755.374 kb on + strand, within PGA1_c16820 at 1755.380 kb on + strand, within PGA1_c16820 at 1755.392 kb on - strand, within PGA1_c16820 at 1755.397 kb on - strand, within PGA1_c16820 at 1755.447 kb on + strand, within PGA1_c16820 at 1755.449 kb on + strand, within PGA1_c16820 at 1755.593 kb on + strand at 1755.601 kb on - strand at 1755.751 kb on + strand, within PGA1_c16830 at 1755.767 kb on + strand, within PGA1_c16830 at 1755.850 kb on + strand, within PGA1_c16830 at 1755.851 kb on + strand, within PGA1_c16830 at 1755.878 kb on - strand, within PGA1_c16830 at 1755.950 kb on + strand, within PGA1_c16830 at 1755.950 kb on + strand, within PGA1_c16830 at 1755.958 kb on - strand, within PGA1_c16830 at 1755.996 kb on + strand, within PGA1_c16830 at 1756.004 kb on - strand, within PGA1_c16830 at 1756.142 kb on - strand at 1756.226 kb on - strand at 1756.234 kb on + strand at 1756.234 kb on + strand at 1756.234 kb on + strand at 1756.236 kb on + strand at 1756.242 kb on - strand at 1756.242 kb on - strand at 1756.242 kb on - strand at 1756.247 kb on + strand at 1756.275 kb on + strand at 1756.278 kb on - strand at 1756.317 kb on + strand at 1756.317 kb on + strand at 1756.317 kb on + strand at 1756.325 kb on - strand at 1756.370 kb on - strand at 1756.371 kb on + strand at 1756.430 kb on + strand at 1756.473 kb on - strand, within PGA1_c16840 at 1756.639 kb on - strand, within PGA1_c16840 at 1756.757 kb on + strand, within PGA1_c16840 at 1756.770 kb on - strand, within PGA1_c16840 at 1756.785 kb on - strand, within PGA1_c16840 at 1756.820 kb on - strand, within PGA1_c16840 at 1756.827 kb on - strand, within PGA1_c16840 at 1756.855 kb on - strand, within PGA1_c16840 at 1756.893 kb on + strand, within PGA1_c16840 at 1756.901 kb on - strand, within PGA1_c16840 at 1756.901 kb on - strand, within PGA1_c16840 at 1756.901 kb on - strand, within PGA1_c16840 at 1756.908 kb on + strand, within PGA1_c16840 at 1756.909 kb on + strand, within PGA1_c16840 at 1756.921 kb on - strand, within PGA1_c16840 at 1757.024 kb on - strand, within PGA1_c16840 at 1757.104 kb on - strand, within PGA1_c16840 at 1757.104 kb on - strand, within PGA1_c16840 at 1757.267 kb on - strand, within PGA1_c16840 at 1757.291 kb on - strand, within PGA1_c16840 at 1757.351 kb on - strand, within PGA1_c16840 at 1757.369 kb on - strand, within PGA1_c16840 at 1757.393 kb on - strand, within PGA1_c16840 at 1757.443 kb on + strand at 1757.474 kb on + strand at 1757.482 kb on - strand at 1757.506 kb on + strand at 1757.519 kb on - strand at 1757.542 kb on + strand at 1757.558 kb on - strand at 1757.558 kb on - strand at 1757.586 kb on - strand at 1757.665 kb on + strand at 1757.665 kb on + strand at 1757.713 kb on + strand at 1758.094 kb on + strand, within PGA1_c16850 at 1758.102 kb on - strand, within PGA1_c16850 at 1758.337 kb on + strand, within PGA1_c16850
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 1,755,335 - PGA1_c16820 0.61 +2.1 1,755,336 - PGA1_c16820 0.62 -0.4 1,755,374 + PGA1_c16820 0.68 -0.8 1,755,380 + PGA1_c16820 0.69 -0.3 1,755,392 - PGA1_c16820 0.71 +1.0 1,755,397 - PGA1_c16820 0.72 +0.5 1,755,447 + PGA1_c16820 0.80 +1.1 1,755,449 + PGA1_c16820 0.80 +0.2 1,755,593 + -0.1 1,755,601 - +0.8 1,755,751 + PGA1_c16830 0.26 -0.6 1,755,767 + PGA1_c16830 0.29 +1.3 1,755,850 + PGA1_c16830 0.45 +0.8 1,755,851 + PGA1_c16830 0.45 +0.8 1,755,878 - PGA1_c16830 0.51 -0.2 1,755,950 + PGA1_c16830 0.65 -1.7 1,755,950 + PGA1_c16830 0.65 -0.5 1,755,958 - PGA1_c16830 0.67 -0.6 1,755,996 + PGA1_c16830 0.74 +0.2 1,756,004 - PGA1_c16830 0.76 -1.2 1,756,142 - +1.1 1,756,226 - -0.3 1,756,234 + -0.4 1,756,234 + +1.7 1,756,234 + +1.1 1,756,236 + -0.8 1,756,242 - -0.4 1,756,242 - -1.4 1,756,242 - -0.2 1,756,247 + +0.5 1,756,275 + -0.1 1,756,278 - +0.2 1,756,317 + -1.6 1,756,317 + +1.1 1,756,317 + +0.2 1,756,325 - +0.2 1,756,370 - -0.2 1,756,371 + +1.4 1,756,430 + +1.0 1,756,473 - PGA1_c16840 0.12 -0.2 1,756,639 - PGA1_c16840 0.26 -0.1 1,756,757 + PGA1_c16840 0.36 +0.4 1,756,770 - PGA1_c16840 0.37 -0.1 1,756,785 - PGA1_c16840 0.38 +1.2 1,756,820 - PGA1_c16840 0.41 +0.1 1,756,827 - PGA1_c16840 0.42 +0.8 1,756,855 - PGA1_c16840 0.44 +0.2 1,756,893 + PGA1_c16840 0.47 -0.7 1,756,901 - PGA1_c16840 0.48 -0.4 1,756,901 - PGA1_c16840 0.48 -0.4 1,756,901 - PGA1_c16840 0.48 +0.8 1,756,908 + PGA1_c16840 0.48 +1.4 1,756,909 + PGA1_c16840 0.49 -1.2 1,756,921 - PGA1_c16840 0.50 +0.5 1,757,024 - PGA1_c16840 0.58 +1.4 1,757,104 - PGA1_c16840 0.65 +0.7 1,757,104 - PGA1_c16840 0.65 +0.1 1,757,267 - PGA1_c16840 0.79 +1.2 1,757,291 - PGA1_c16840 0.81 +0.4 1,757,351 - PGA1_c16840 0.86 -0.6 1,757,369 - PGA1_c16840 0.87 +2.8 1,757,393 - PGA1_c16840 0.89 +0.1 1,757,443 + +0.7 1,757,474 + +1.8 1,757,482 - -0.6 1,757,506 + -2.1 1,757,519 - +0.4 1,757,542 + +0.5 1,757,558 - +0.4 1,757,558 - -0.5 1,757,586 - -1.3 1,757,665 + -1.4 1,757,665 + -0.6 1,757,713 + +1.4 1,758,094 + PGA1_c16850 0.23 -0.2 1,758,102 - PGA1_c16850 0.24 -1.8 1,758,337 + PGA1_c16850 0.36 -1.4
Or see this region's nucleotide sequence