Experiment: DinoMM minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c16050 and PGA1_c16060 are separated by 26 nucleotides PGA1_c16060 and PGA1_c16070 are separated by 324 nucleotides PGA1_c16070 and PGA1_c16080 are separated by 70 nucleotides
PGA1_c16050: PGA1_c16050 - 5,10-methylenetetrahydrofolate reductase, at 1,665,163 to 1,666,095
_c16050
PGA1_c16060: PGA1_c16060 - hypothetical protein, at 1,666,122 to 1,666,418
_c16060
PGA1_c16070: PGA1_c16070 - guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase-like protein, at 1,666,743 to 1,667,855
_c16070
PGA1_c16080: PGA1_c16080 - ribosomal RNA large subunit methyltransferase J, at 1,667,926 to 1,668,681
_c16080
Position (kb)
1666
1667
1668 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1665.785 kb on + strand, within PGA1_c16050 at 1665.932 kb on + strand, within PGA1_c16050 at 1665.992 kb on + strand, within PGA1_c16050 at 1666.056 kb on - strand at 1666.061 kb on - strand at 1666.104 kb on + strand at 1666.152 kb on + strand, within PGA1_c16060 at 1666.152 kb on + strand, within PGA1_c16060 at 1666.160 kb on - strand, within PGA1_c16060 at 1666.161 kb on + strand, within PGA1_c16060 at 1666.218 kb on - strand, within PGA1_c16060 at 1666.229 kb on - strand, within PGA1_c16060 at 1666.248 kb on + strand, within PGA1_c16060 at 1666.305 kb on + strand, within PGA1_c16060 at 1666.379 kb on - strand, within PGA1_c16060 at 1666.395 kb on + strand at 1666.420 kb on + strand at 1666.506 kb on - strand at 1666.555 kb on - strand at 1666.555 kb on - strand at 1666.555 kb on - strand at 1666.560 kb on - strand at 1666.562 kb on + strand at 1666.591 kb on - strand at 1666.623 kb on - strand at 1666.668 kb on + strand at 1666.668 kb on + strand at 1666.678 kb on - strand at 1666.687 kb on - strand at 1666.687 kb on - strand at 1666.689 kb on - strand at 1666.711 kb on - strand at 1666.726 kb on + strand at 1666.765 kb on - strand at 1666.765 kb on - strand at 1666.795 kb on + strand at 1666.805 kb on + strand at 1666.952 kb on + strand, within PGA1_c16070 at 1667.041 kb on - strand, within PGA1_c16070 at 1667.336 kb on - strand, within PGA1_c16070 at 1667.499 kb on - strand, within PGA1_c16070 at 1667.577 kb on + strand, within PGA1_c16070 at 1667.594 kb on + strand, within PGA1_c16070 at 1667.712 kb on + strand, within PGA1_c16070 at 1667.866 kb on + strand at 1667.868 kb on + strand at 1668.670 kb on + strand at 1668.717 kb on + strand at 1668.720 kb on - strand at 1668.722 kb on + strand at 1668.730 kb on - strand at 1668.762 kb on + strand at 1668.770 kb on - strand at 1668.806 kb on - strand at 1668.842 kb on - strand at 1668.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media remove 1,665,785 + PGA1_c16050 0.67 -2.4 1,665,932 + PGA1_c16050 0.82 -2.4 1,665,992 + PGA1_c16050 0.89 -3.2 1,666,056 - -3.3 1,666,061 - -1.9 1,666,104 + -1.4 1,666,152 + PGA1_c16060 0.10 -0.6 1,666,152 + PGA1_c16060 0.10 -0.6 1,666,160 - PGA1_c16060 0.13 -1.2 1,666,161 + PGA1_c16060 0.13 +0.4 1,666,218 - PGA1_c16060 0.32 -3.7 1,666,229 - PGA1_c16060 0.36 -0.9 1,666,248 + PGA1_c16060 0.42 -2.9 1,666,305 + PGA1_c16060 0.62 +0.4 1,666,379 - PGA1_c16060 0.87 -3.3 1,666,395 + -1.3 1,666,420 + -1.9 1,666,506 - -2.8 1,666,555 - +1.4 1,666,555 - +0.5 1,666,555 - +0.7 1,666,560 - -1.4 1,666,562 + +0.9 1,666,591 - -1.0 1,666,623 - +1.0 1,666,668 + +0.1 1,666,668 + +0.1 1,666,678 - -0.8 1,666,687 - -1.1 1,666,687 - -0.2 1,666,689 - -0.6 1,666,711 - +1.5 1,666,726 + -1.2 1,666,765 - +0.3 1,666,765 - -1.6 1,666,795 + -0.6 1,666,805 + +0.4 1,666,952 + PGA1_c16070 0.19 -1.4 1,667,041 - PGA1_c16070 0.27 -2.2 1,667,336 - PGA1_c16070 0.53 +0.1 1,667,499 - PGA1_c16070 0.68 -2.1 1,667,577 + PGA1_c16070 0.75 -2.3 1,667,594 + PGA1_c16070 0.76 -1.0 1,667,712 + PGA1_c16070 0.87 -2.0 1,667,866 + -0.4 1,667,868 + +0.4 1,668,670 + -0.8 1,668,717 + -0.4 1,668,720 - +0.6 1,668,722 + +0.6 1,668,730 - +0.4 1,668,762 + -0.1 1,668,770 - -0.2 1,668,806 - +0.7 1,668,842 - -0.8 1,668,850 - +0.2
Or see this region's nucleotide sequence