Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22105 and BWI76_RS22110 overlap by 22 nucleotides BWI76_RS22110 and BWI76_RS22115 are separated by 57 nucleotides BWI76_RS22115 and BWI76_RS22120 are separated by 165 nucleotides
BWI76_RS22105: BWI76_RS22105 - 2-isopropylmalate synthase, at 4,614,684 to 4,616,360
_RS22105
BWI76_RS22110: BWI76_RS22110 - putative helix-turn-helix AraC-type transcriptional regulator, at 4,616,339 to 4,617,106
_RS22110
BWI76_RS22115: BWI76_RS22115 - S-ribosylhomocysteine lyase, at 4,617,164 to 4,617,679
_RS22115
BWI76_RS22120: BWI76_RS22120 - glutamate--cysteine ligase, at 4,617,845 to 4,619,401
_RS22120
Position (kb) 4617
4618 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7 at 4616.446 kb on + strand, within BWI76_RS22110 at 4616.842 kb on + strand, within BWI76_RS22110 at 4616.860 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.895 kb on - strand, within BWI76_RS22110 at 4616.895 kb on - strand, within BWI76_RS22110 at 4616.969 kb on - strand, within BWI76_RS22110 at 4617.301 kb on + strand, within BWI76_RS22115 at 4617.473 kb on + strand, within BWI76_RS22115 at 4617.598 kb on - strand, within BWI76_RS22115 at 4617.625 kb on + strand, within BWI76_RS22115 at 4617.671 kb on - strand at 4617.737 kb on + strand at 4617.755 kb on - strand at 4617.987 kb on - strand at 4618.294 kb on + strand, within BWI76_RS22120 at 4618.434 kb on - strand, within BWI76_RS22120 at 4618.677 kb on + strand, within BWI76_RS22120
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,616,446 + BWI76_RS22110 0.14 -0.2 4,616,842 + BWI76_RS22110 0.65 +0.2 4,616,860 + BWI76_RS22110 0.68 -0.0 4,616,887 + BWI76_RS22110 0.71 +0.6 4,616,887 + BWI76_RS22110 0.71 +1.3 4,616,887 + BWI76_RS22110 0.71 +0.7 4,616,895 - BWI76_RS22110 0.72 +0.2 4,616,895 - BWI76_RS22110 0.72 -1.0 4,616,969 - BWI76_RS22110 0.82 +0.3 4,617,301 + BWI76_RS22115 0.27 +0.5 4,617,473 + BWI76_RS22115 0.60 +0.5 4,617,598 - BWI76_RS22115 0.84 +0.4 4,617,625 + BWI76_RS22115 0.89 +0.1 4,617,671 - -0.0 4,617,737 + +0.3 4,617,755 - -0.4 4,617,987 - +6.8 4,618,294 + BWI76_RS22120 0.29 +7.1 4,618,434 - BWI76_RS22120 0.38 +7.3 4,618,677 + BWI76_RS22120 0.53 +7.3
Or see this region's nucleotide sequence