Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05480 and PGA1_c05490 are separated by 43 nucleotides PGA1_c05490 and PGA1_c05500 are separated by 135 nucleotides PGA1_c05500 and PGA1_c05510 are separated by 105 nucleotides
PGA1_c05480: PGA1_c05480 - OHCU decarboxylase domain-containing protein, at 559,611 to 561,026
_c05480
PGA1_c05490: PGA1_c05490 - ureidoglycolate hydrolase AllA, at 561,070 to 561,555
_c05490
PGA1_c05500: PGA1_c05500 - putative purine permease, at 561,691 to 563,118
_c05500
PGA1_c05510: PGA1_c05510 - glyceraldehyde-3-phosphate dehydrogenase 1, at 563,224 to 564,285
_c05510
Position (kb) 561
562
563
564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 560.714 kb on - strand, within PGA1_c05480 at 560.757 kb on - strand, within PGA1_c05480 at 560.779 kb on - strand, within PGA1_c05480 at 560.786 kb on + strand, within PGA1_c05480 at 560.838 kb on - strand, within PGA1_c05480 at 560.923 kb on + strand at 560.923 kb on + strand at 560.923 kb on + strand at 560.923 kb on + strand at 560.923 kb on + strand at 560.931 kb on - strand at 560.931 kb on - strand at 560.931 kb on - strand at 561.023 kb on - strand at 561.025 kb on + strand at 561.028 kb on - strand at 561.038 kb on + strand at 561.046 kb on - strand at 561.106 kb on - strand at 561.107 kb on + strand at 561.168 kb on + strand, within PGA1_c05490 at 561.207 kb on - strand, within PGA1_c05490 at 561.237 kb on + strand, within PGA1_c05490 at 561.261 kb on + strand, within PGA1_c05490 at 561.284 kb on - strand, within PGA1_c05490 at 561.307 kb on + strand, within PGA1_c05490 at 561.334 kb on - strand, within PGA1_c05490 at 561.350 kb on + strand, within PGA1_c05490 at 561.355 kb on + strand, within PGA1_c05490 at 561.375 kb on - strand, within PGA1_c05490 at 561.416 kb on + strand, within PGA1_c05490 at 561.416 kb on + strand, within PGA1_c05490 at 561.434 kb on + strand, within PGA1_c05490 at 561.514 kb on + strand at 561.530 kb on + strand at 561.583 kb on - strand at 561.645 kb on - strand at 561.689 kb on - strand at 561.689 kb on - strand at 561.689 kb on - strand at 561.713 kb on + strand at 561.753 kb on + strand at 561.753 kb on + strand at 561.753 kb on + strand at 561.761 kb on - strand at 561.761 kb on - strand at 561.789 kb on + strand at 561.792 kb on - strand at 561.797 kb on - strand at 561.797 kb on - strand at 561.853 kb on + strand, within PGA1_c05500 at 561.853 kb on + strand, within PGA1_c05500 at 561.853 kb on + strand, within PGA1_c05500 at 561.853 kb on + strand, within PGA1_c05500 at 561.853 kb on + strand, within PGA1_c05500 at 561.861 kb on - strand, within PGA1_c05500 at 561.861 kb on - strand, within PGA1_c05500 at 561.914 kb on - strand, within PGA1_c05500 at 561.920 kb on + strand, within PGA1_c05500 at 561.976 kb on + strand, within PGA1_c05500 at 561.979 kb on - strand, within PGA1_c05500 at 561.984 kb on - strand, within PGA1_c05500 at 562.037 kb on - strand, within PGA1_c05500 at 562.056 kb on + strand, within PGA1_c05500 at 562.056 kb on + strand, within PGA1_c05500 at 562.056 kb on + strand, within PGA1_c05500 at 562.124 kb on - strand, within PGA1_c05500 at 562.169 kb on + strand, within PGA1_c05500 at 562.169 kb on + strand, within PGA1_c05500 at 562.179 kb on + strand, within PGA1_c05500 at 562.187 kb on - strand, within PGA1_c05500 at 562.256 kb on + strand, within PGA1_c05500 at 562.286 kb on + strand, within PGA1_c05500 at 562.286 kb on + strand, within PGA1_c05500 at 562.291 kb on + strand, within PGA1_c05500 at 562.383 kb on + strand, within PGA1_c05500 at 562.383 kb on + strand, within PGA1_c05500 at 562.391 kb on - strand, within PGA1_c05500 at 562.420 kb on + strand, within PGA1_c05500 at 562.427 kb on + strand, within PGA1_c05500 at 562.435 kb on - strand, within PGA1_c05500 at 562.481 kb on + strand, within PGA1_c05500 at 562.509 kb on - strand, within PGA1_c05500 at 562.641 kb on - strand, within PGA1_c05500 at 562.647 kb on + strand, within PGA1_c05500 at 562.647 kb on + strand, within PGA1_c05500 at 562.664 kb on + strand, within PGA1_c05500 at 562.683 kb on - strand, within PGA1_c05500 at 562.739 kb on - strand, within PGA1_c05500 at 562.755 kb on + strand, within PGA1_c05500 at 562.918 kb on - strand, within PGA1_c05500 at 562.945 kb on + strand, within PGA1_c05500 at 562.953 kb on - strand, within PGA1_c05500 at 562.956 kb on + strand, within PGA1_c05500 at 562.982 kb on + strand at 562.982 kb on + strand at 562.982 kb on + strand at 562.990 kb on - strand at 562.990 kb on - strand at 562.992 kb on + strand at 563.012 kb on + strand at 563.017 kb on + strand at 563.017 kb on + strand at 563.025 kb on - strand at 563.086 kb on + strand at 563.092 kb on + strand at 563.099 kb on - strand at 563.123 kb on + strand at 563.123 kb on + strand at 563.128 kb on + strand at 563.133 kb on + strand at 563.135 kb on - strand at 563.136 kb on - strand at 563.376 kb on + strand, within PGA1_c05510 at 563.376 kb on + strand, within PGA1_c05510 at 563.381 kb on + strand, within PGA1_c05510 at 563.384 kb on - strand, within PGA1_c05510 at 563.384 kb on - strand, within PGA1_c05510 at 563.410 kb on - strand, within PGA1_c05510 at 563.410 kb on - strand, within PGA1_c05510 at 563.488 kb on - strand, within PGA1_c05510 at 563.649 kb on + strand, within PGA1_c05510 at 563.734 kb on - strand, within PGA1_c05510 at 563.734 kb on - strand, within PGA1_c05510 at 563.735 kb on + strand, within PGA1_c05510 at 563.782 kb on - strand, within PGA1_c05510 at 563.831 kb on - strand, within PGA1_c05510 at 563.860 kb on + strand, within PGA1_c05510 at 563.904 kb on + strand, within PGA1_c05510 at 563.904 kb on + strand, within PGA1_c05510 at 563.958 kb on + strand, within PGA1_c05510 at 563.981 kb on - strand, within PGA1_c05510 at 563.981 kb on - strand, within PGA1_c05510 at 564.026 kb on - strand, within PGA1_c05510 at 564.026 kb on - strand, within PGA1_c05510 at 564.054 kb on + strand, within PGA1_c05510 at 564.062 kb on + strand, within PGA1_c05510 at 564.062 kb on - strand, within PGA1_c05510 at 564.102 kb on - strand, within PGA1_c05510
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 560,714 - PGA1_c05480 0.78 -0.2 560,757 - PGA1_c05480 0.81 -0.4 560,779 - PGA1_c05480 0.82 -1.0 560,786 + PGA1_c05480 0.83 -0.6 560,838 - PGA1_c05480 0.87 +1.1 560,923 + +1.4 560,923 + -1.3 560,923 + +0.2 560,923 + -0.0 560,923 + +0.6 560,931 - +0.6 560,931 - +0.0 560,931 - +0.4 561,023 - +1.3 561,025 + -0.2 561,028 - +1.6 561,038 + -1.6 561,046 - +0.0 561,106 - -0.4 561,107 + -1.0 561,168 + PGA1_c05490 0.20 -1.0 561,207 - PGA1_c05490 0.28 +4.3 561,237 + PGA1_c05490 0.34 -0.5 561,261 + PGA1_c05490 0.39 -0.5 561,284 - PGA1_c05490 0.44 -0.0 561,307 + PGA1_c05490 0.49 +1.6 561,334 - PGA1_c05490 0.54 +1.0 561,350 + PGA1_c05490 0.58 +0.0 561,355 + PGA1_c05490 0.59 -0.4 561,375 - PGA1_c05490 0.63 -0.3 561,416 + PGA1_c05490 0.71 +0.3 561,416 + PGA1_c05490 0.71 +0.9 561,434 + PGA1_c05490 0.75 +1.8 561,514 + -1.1 561,530 + +1.6 561,583 - -0.9 561,645 - -0.2 561,689 - +0.5 561,689 - +1.6 561,689 - +0.5 561,713 + +0.2 561,753 + -1.0 561,753 + -1.7 561,753 + +1.0 561,761 - +0.9 561,761 - -1.5 561,789 + -0.9 561,792 - -0.1 561,797 - +2.5 561,797 - -0.1 561,853 + PGA1_c05500 0.11 -1.0 561,853 + PGA1_c05500 0.11 -1.3 561,853 + PGA1_c05500 0.11 +0.8 561,853 + PGA1_c05500 0.11 +0.8 561,853 + PGA1_c05500 0.11 +1.5 561,861 - PGA1_c05500 0.12 +0.6 561,861 - PGA1_c05500 0.12 +0.5 561,914 - PGA1_c05500 0.16 -1.3 561,920 + PGA1_c05500 0.16 +0.3 561,976 + PGA1_c05500 0.20 +1.3 561,979 - PGA1_c05500 0.20 -0.5 561,984 - PGA1_c05500 0.21 +0.1 562,037 - PGA1_c05500 0.24 +0.1 562,056 + PGA1_c05500 0.26 +0.7 562,056 + PGA1_c05500 0.26 -1.5 562,056 + PGA1_c05500 0.26 -1.6 562,124 - PGA1_c05500 0.30 -1.4 562,169 + PGA1_c05500 0.33 -1.2 562,169 + PGA1_c05500 0.33 +0.7 562,179 + PGA1_c05500 0.34 +1.0 562,187 - PGA1_c05500 0.35 -0.9 562,256 + PGA1_c05500 0.40 +0.9 562,286 + PGA1_c05500 0.42 +1.7 562,286 + PGA1_c05500 0.42 -0.6 562,291 + PGA1_c05500 0.42 +1.0 562,383 + PGA1_c05500 0.48 +0.3 562,383 + PGA1_c05500 0.48 +1.1 562,391 - PGA1_c05500 0.49 +0.3 562,420 + PGA1_c05500 0.51 -1.5 562,427 + PGA1_c05500 0.52 -0.9 562,435 - PGA1_c05500 0.52 +0.8 562,481 + PGA1_c05500 0.55 -0.3 562,509 - PGA1_c05500 0.57 +0.9 562,641 - PGA1_c05500 0.67 +0.1 562,647 + PGA1_c05500 0.67 +0.1 562,647 + PGA1_c05500 0.67 +2.3 562,664 + PGA1_c05500 0.68 -0.0 562,683 - PGA1_c05500 0.69 -2.2 562,739 - PGA1_c05500 0.73 +0.6 562,755 + PGA1_c05500 0.75 -2.6 562,918 - PGA1_c05500 0.86 -0.1 562,945 + PGA1_c05500 0.88 +0.5 562,953 - PGA1_c05500 0.88 -0.5 562,956 + PGA1_c05500 0.89 +1.2 562,982 + -0.7 562,982 + +0.1 562,982 + +0.9 562,990 - +0.9 562,990 - +0.3 562,992 + +0.7 563,012 + -0.3 563,017 + -0.2 563,017 + +1.6 563,025 - -0.2 563,086 + -1.0 563,092 + +0.7 563,099 - -0.8 563,123 + +0.7 563,123 + +0.5 563,128 + -0.7 563,133 + -0.2 563,135 - +1.2 563,136 - -1.7 563,376 + PGA1_c05510 0.14 -1.3 563,376 + PGA1_c05510 0.14 +1.0 563,381 + PGA1_c05510 0.15 -1.4 563,384 - PGA1_c05510 0.15 -0.5 563,384 - PGA1_c05510 0.15 -0.7 563,410 - PGA1_c05510 0.18 +0.3 563,410 - PGA1_c05510 0.18 +0.7 563,488 - PGA1_c05510 0.25 -1.5 563,649 + PGA1_c05510 0.40 +0.3 563,734 - PGA1_c05510 0.48 -1.3 563,734 - PGA1_c05510 0.48 -0.5 563,735 + PGA1_c05510 0.48 -2.7 563,782 - PGA1_c05510 0.53 +0.1 563,831 - PGA1_c05510 0.57 -1.6 563,860 + PGA1_c05510 0.60 -0.3 563,904 + PGA1_c05510 0.64 -1.9 563,904 + PGA1_c05510 0.64 -2.8 563,958 + PGA1_c05510 0.69 -0.9 563,981 - PGA1_c05510 0.71 -1.4 563,981 - PGA1_c05510 0.71 -0.9 564,026 - PGA1_c05510 0.76 -2.3 564,026 - PGA1_c05510 0.76 -2.0 564,054 + PGA1_c05510 0.78 -2.5 564,062 + PGA1_c05510 0.79 -0.2 564,062 - PGA1_c05510 0.79 -3.1 564,102 - PGA1_c05510 0.83 -0.5
Or see this region's nucleotide sequence