Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03910 and PGA1_c03920 are separated by 95 nucleotides PGA1_c03920 and PGA1_c03930 are separated by 1 nucleotides PGA1_c03930 and PGA1_c03940 are separated by 234 nucleotides
PGA1_c03910: PGA1_c03910 - short chain dehydrogenase / reductase, at 377,794 to 378,552
_c03910
PGA1_c03920: PGA1_c03920 - Putative transmembrane protein (Alph_Pro_TM)., at 378,648 to 379,538
_c03920
PGA1_c03930: PGA1_c03930 - Predicted permeases, at 379,540 to 380,463
_c03930
PGA1_c03940: PGA1_c03940 - putative permease protein, at 380,698 to 381,534
_c03940
Position (kb) 379
380
381 Strain fitness (log2 ratio) -2
-1
0
1
2 at 378.626 kb on + strand at 378.644 kb on - strand at 378.725 kb on - strand at 378.773 kb on + strand, within PGA1_c03920 at 378.773 kb on + strand, within PGA1_c03920 at 378.933 kb on - strand, within PGA1_c03920 at 379.089 kb on - strand, within PGA1_c03920 at 379.144 kb on - strand, within PGA1_c03920 at 379.157 kb on + strand, within PGA1_c03920 at 379.237 kb on + strand, within PGA1_c03920 at 379.482 kb on + strand at 379.482 kb on + strand at 379.490 kb on - strand at 379.615 kb on + strand at 379.626 kb on + strand at 379.634 kb on - strand, within PGA1_c03930 at 379.750 kb on + strand, within PGA1_c03930 at 379.826 kb on - strand, within PGA1_c03930 at 379.929 kb on - strand, within PGA1_c03930 at 379.934 kb on - strand, within PGA1_c03930 at 379.963 kb on - strand, within PGA1_c03930 at 380.125 kb on - strand, within PGA1_c03930 at 380.234 kb on - strand, within PGA1_c03930 at 380.468 kb on + strand at 380.468 kb on + strand at 380.476 kb on - strand at 380.476 kb on - strand at 380.476 kb on - strand at 380.476 kb on - strand at 380.476 kb on - strand at 380.614 kb on - strand at 380.638 kb on - strand at 380.665 kb on + strand at 380.665 kb on + strand at 380.716 kb on + strand at 380.716 kb on + strand at 380.729 kb on + strand at 380.777 kb on + strand at 380.783 kb on + strand, within PGA1_c03940 at 380.803 kb on + strand, within PGA1_c03940 at 380.861 kb on + strand, within PGA1_c03940 at 380.960 kb on + strand, within PGA1_c03940 at 380.960 kb on + strand, within PGA1_c03940 at 380.968 kb on - strand, within PGA1_c03940 at 380.980 kb on + strand, within PGA1_c03940 at 381.110 kb on - strand, within PGA1_c03940 at 381.179 kb on + strand, within PGA1_c03940 at 381.183 kb on - strand, within PGA1_c03940 at 381.188 kb on + strand, within PGA1_c03940 at 381.276 kb on + strand, within PGA1_c03940 at 381.290 kb on + strand, within PGA1_c03940 at 381.290 kb on + strand, within PGA1_c03940 at 381.298 kb on - strand, within PGA1_c03940 at 381.314 kb on - strand, within PGA1_c03940 at 381.326 kb on + strand, within PGA1_c03940 at 381.339 kb on - strand, within PGA1_c03940 at 381.352 kb on + strand, within PGA1_c03940 at 381.391 kb on - strand, within PGA1_c03940
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 378,626 + -0.1 378,644 - +0.4 378,725 - +0.6 378,773 + PGA1_c03920 0.14 +2.3 378,773 + PGA1_c03920 0.14 -1.0 378,933 - PGA1_c03920 0.32 +0.6 379,089 - PGA1_c03920 0.49 -1.0 379,144 - PGA1_c03920 0.56 -0.2 379,157 + PGA1_c03920 0.57 +0.3 379,237 + PGA1_c03920 0.66 -0.3 379,482 + -1.0 379,482 + +0.3 379,490 - +0.3 379,615 + -0.5 379,626 + -0.3 379,634 - PGA1_c03930 0.10 -0.7 379,750 + PGA1_c03930 0.23 -1.3 379,826 - PGA1_c03930 0.31 -1.0 379,929 - PGA1_c03930 0.42 -1.0 379,934 - PGA1_c03930 0.43 -1.7 379,963 - PGA1_c03930 0.46 -1.0 380,125 - PGA1_c03930 0.63 -1.3 380,234 - PGA1_c03930 0.75 +0.9 380,468 + +0.7 380,468 + -0.3 380,476 - -0.6 380,476 - +2.3 380,476 - -0.7 380,476 - -0.9 380,476 - -0.0 380,614 - +1.3 380,638 - -0.7 380,665 + -0.1 380,665 + +0.2 380,716 + -0.0 380,716 + +0.4 380,729 + -0.3 380,777 + -1.6 380,783 + PGA1_c03940 0.10 -2.3 380,803 + PGA1_c03940 0.13 -1.9 380,861 + PGA1_c03940 0.19 -0.1 380,960 + PGA1_c03940 0.31 -0.1 380,960 + PGA1_c03940 0.31 -1.8 380,968 - PGA1_c03940 0.32 -2.1 380,980 + PGA1_c03940 0.34 +0.1 381,110 - PGA1_c03940 0.49 +1.1 381,179 + PGA1_c03940 0.57 -2.5 381,183 - PGA1_c03940 0.58 -0.5 381,188 + PGA1_c03940 0.59 -0.5 381,276 + PGA1_c03940 0.69 +1.3 381,290 + PGA1_c03940 0.71 -0.3 381,290 + PGA1_c03940 0.71 -0.3 381,298 - PGA1_c03940 0.72 +0.2 381,314 - PGA1_c03940 0.74 -2.3 381,326 + PGA1_c03940 0.75 -0.4 381,339 - PGA1_c03940 0.77 -0.1 381,352 + PGA1_c03940 0.78 -0.6 381,391 - PGA1_c03940 0.83 +0.3
Or see this region's nucleotide sequence