Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00770 and PGA1_262p00780 are separated by 4 nucleotides PGA1_262p00780 and PGA1_262p00790 are separated by 97 nucleotides PGA1_262p00790 and PGA1_262p00800 are separated by 103 nucleotides
PGA1_262p00770: PGA1_262p00770 - putative lytic murein transglycosylase, at 86,081 to 87,325
_262p00770
PGA1_262p00780: PGA1_262p00780 - NAD dependent epimerase/dehydratase, at 87,330 to 88,439
_262p00780
PGA1_262p00790: PGA1_262p00790 - hypothetical protein, at 88,537 to 88,668
_262p00790
PGA1_262p00800: PGA1_262p00800 - phenylacetic acid degradation protein PaaZ, at 88,772 to 90,904
_262p00800
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 86.512 kb on + strand, within PGA1_262p00770 at 86.556 kb on + strand, within PGA1_262p00770 at 86.635 kb on + strand, within PGA1_262p00770 at 86.906 kb on - strand, within PGA1_262p00770 at 86.928 kb on - strand, within PGA1_262p00770 at 87.234 kb on - strand at 87.306 kb on + strand at 87.452 kb on + strand, within PGA1_262p00780 at 87.452 kb on + strand, within PGA1_262p00780 at 87.452 kb on + strand, within PGA1_262p00780 at 87.452 kb on + strand, within PGA1_262p00780 at 87.452 kb on + strand, within PGA1_262p00780 at 87.460 kb on - strand, within PGA1_262p00780 at 87.520 kb on + strand, within PGA1_262p00780 at 87.596 kb on + strand, within PGA1_262p00780 at 87.598 kb on + strand, within PGA1_262p00780 at 87.641 kb on + strand, within PGA1_262p00780 at 87.746 kb on + strand, within PGA1_262p00780 at 87.823 kb on + strand, within PGA1_262p00780 at 87.833 kb on + strand, within PGA1_262p00780 at 87.911 kb on + strand, within PGA1_262p00780 at 87.954 kb on + strand, within PGA1_262p00780 at 87.973 kb on + strand, within PGA1_262p00780 at 88.026 kb on - strand, within PGA1_262p00780 at 88.026 kb on - strand, within PGA1_262p00780 at 88.052 kb on + strand, within PGA1_262p00780 at 88.441 kb on + strand at 88.721 kb on + strand at 88.764 kb on + strand at 88.764 kb on + strand at 88.772 kb on - strand at 88.772 kb on - strand at 88.861 kb on - strand at 88.879 kb on + strand at 88.892 kb on - strand at 88.903 kb on - strand at 88.943 kb on + strand at 88.988 kb on + strand, within PGA1_262p00800 at 89.033 kb on + strand, within PGA1_262p00800 at 89.071 kb on + strand, within PGA1_262p00800 at 89.110 kb on + strand, within PGA1_262p00800 at 89.122 kb on + strand, within PGA1_262p00800 at 89.132 kb on + strand, within PGA1_262p00800 at 89.141 kb on + strand, within PGA1_262p00800 at 89.159 kb on + strand, within PGA1_262p00800 at 89.232 kb on + strand, within PGA1_262p00800 at 89.240 kb on - strand, within PGA1_262p00800 at 89.240 kb on - strand, within PGA1_262p00800 at 89.438 kb on + strand, within PGA1_262p00800
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 86,512 + PGA1_262p00770 0.35 +2.4 86,556 + PGA1_262p00770 0.38 -0.5 86,635 + PGA1_262p00770 0.44 -0.0 86,906 - PGA1_262p00770 0.66 +1.7 86,928 - PGA1_262p00770 0.68 +1.1 87,234 - -0.2 87,306 + -0.7 87,452 + PGA1_262p00780 0.11 +0.3 87,452 + PGA1_262p00780 0.11 +0.1 87,452 + PGA1_262p00780 0.11 +0.1 87,452 + PGA1_262p00780 0.11 +0.2 87,452 + PGA1_262p00780 0.11 +0.1 87,460 - PGA1_262p00780 0.12 +0.1 87,520 + PGA1_262p00780 0.17 -0.0 87,596 + PGA1_262p00780 0.24 +1.4 87,598 + PGA1_262p00780 0.24 -0.5 87,641 + PGA1_262p00780 0.28 +0.8 87,746 + PGA1_262p00780 0.37 -0.7 87,823 + PGA1_262p00780 0.44 -1.2 87,833 + PGA1_262p00780 0.45 -2.5 87,911 + PGA1_262p00780 0.52 -0.3 87,954 + PGA1_262p00780 0.56 -1.7 87,973 + PGA1_262p00780 0.58 -0.6 88,026 - PGA1_262p00780 0.63 +0.3 88,026 - PGA1_262p00780 0.63 -2.7 88,052 + PGA1_262p00780 0.65 +0.1 88,441 + +1.9 88,721 + -1.1 88,764 + +0.7 88,764 + -0.3 88,772 - -0.5 88,772 - +0.6 88,861 - -1.2 88,879 + +1.1 88,892 - -0.2 88,903 - -0.1 88,943 + -0.0 88,988 + PGA1_262p00800 0.10 -0.5 89,033 + PGA1_262p00800 0.12 +0.0 89,071 + PGA1_262p00800 0.14 -0.5 89,110 + PGA1_262p00800 0.16 -2.2 89,122 + PGA1_262p00800 0.16 +0.3 89,132 + PGA1_262p00800 0.17 -0.7 89,141 + PGA1_262p00800 0.17 +0.7 89,159 + PGA1_262p00800 0.18 -0.6 89,232 + PGA1_262p00800 0.22 +0.6 89,240 - PGA1_262p00800 0.22 +0.1 89,240 - PGA1_262p00800 0.22 +0.7 89,438 + PGA1_262p00800 0.31 +0.9
Or see this region's nucleotide sequence