Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c34600 and PGA1_c34610 overlap by 4 nucleotides PGA1_c34610 and PGA1_c34620 are separated by 404 nucleotides
PGA1_c34600: PGA1_c34600 - arginine biosynthesis bifunctional protein ArgJ, at 3,630,495 to 3,631,724
_c34600
PGA1_c34610: PGA1_c34610 - putative mutator MutT protein, at 3,631,721 to 3,632,119
_c34610
PGA1_c34620: PGA1_c34620 - translation initiation factor IF-2, at 3,632,524 to 3,635,007
_c34620
Position (kb)
3631
3632
3633 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3630.741 kb on + strand, within PGA1_c34600 at 3630.797 kb on - strand, within PGA1_c34600 at 3630.797 kb on - strand, within PGA1_c34600 at 3630.812 kb on + strand, within PGA1_c34600 at 3630.820 kb on - strand, within PGA1_c34600 at 3630.862 kb on - strand, within PGA1_c34600 at 3630.949 kb on - strand, within PGA1_c34600 at 3630.966 kb on + strand, within PGA1_c34600 at 3631.050 kb on - strand, within PGA1_c34600 at 3631.073 kb on + strand, within PGA1_c34600 at 3631.188 kb on + strand, within PGA1_c34600 at 3631.196 kb on - strand, within PGA1_c34600 at 3631.234 kb on - strand, within PGA1_c34600 at 3631.391 kb on + strand, within PGA1_c34600 at 3631.430 kb on + strand, within PGA1_c34600 at 3631.438 kb on - strand, within PGA1_c34600 at 3631.455 kb on + strand, within PGA1_c34600 at 3631.569 kb on - strand, within PGA1_c34600 at 3631.663 kb on - strand at 3631.663 kb on - strand at 3631.665 kb on + strand at 3631.665 kb on + strand at 3631.672 kb on + strand at 3631.672 kb on - strand at 3631.710 kb on + strand at 3631.738 kb on - strand at 3631.767 kb on + strand, within PGA1_c34610 at 3631.775 kb on - strand, within PGA1_c34610 at 3631.775 kb on - strand, within PGA1_c34610 at 3631.803 kb on - strand, within PGA1_c34610 at 3631.855 kb on - strand, within PGA1_c34610 at 3631.966 kb on + strand, within PGA1_c34610 at 3631.974 kb on - strand, within PGA1_c34610 at 3631.974 kb on - strand, within PGA1_c34610 at 3631.974 kb on - strand, within PGA1_c34610 at 3631.974 kb on - strand, within PGA1_c34610 at 3632.035 kb on - strand, within PGA1_c34610 at 3632.065 kb on + strand, within PGA1_c34610 at 3632.112 kb on + strand at 3632.172 kb on - strand at 3632.183 kb on - strand at 3632.221 kb on + strand at 3632.229 kb on - strand at 3632.229 kb on - strand at 3632.260 kb on + strand at 3632.285 kb on - strand at 3632.290 kb on + strand at 3632.290 kb on - strand at 3632.319 kb on - strand at 3632.321 kb on + strand at 3632.329 kb on - strand at 3632.329 kb on - strand at 3632.350 kb on - strand at 3632.396 kb on + strand at 3632.402 kb on - strand at 3632.417 kb on + strand at 3632.419 kb on + strand at 3632.419 kb on + strand at 3632.419 kb on + strand at 3632.425 kb on - strand at 3632.427 kb on - strand at 3632.443 kb on + strand at 3632.451 kb on - strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.453 kb on + strand at 3632.456 kb on - strand at 3632.461 kb on - strand at 3632.461 kb on - strand at 3632.461 kb on - strand at 3632.461 kb on - strand at 3632.461 kb on - strand at 3632.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 3,630,741 + PGA1_c34600 0.20 -1.7 3,630,797 - PGA1_c34600 0.25 -2.0 3,630,797 - PGA1_c34600 0.25 -0.9 3,630,812 + PGA1_c34600 0.26 -1.0 3,630,820 - PGA1_c34600 0.26 -0.1 3,630,862 - PGA1_c34600 0.30 -2.8 3,630,949 - PGA1_c34600 0.37 -1.1 3,630,966 + PGA1_c34600 0.38 -0.9 3,631,050 - PGA1_c34600 0.45 -3.3 3,631,073 + PGA1_c34600 0.47 -0.9 3,631,188 + PGA1_c34600 0.56 -4.1 3,631,196 - PGA1_c34600 0.57 -2.0 3,631,234 - PGA1_c34600 0.60 -3.2 3,631,391 + PGA1_c34600 0.73 -2.1 3,631,430 + PGA1_c34600 0.76 -2.6 3,631,438 - PGA1_c34600 0.77 -2.8 3,631,455 + PGA1_c34600 0.78 -1.7 3,631,569 - PGA1_c34600 0.87 -2.0 3,631,663 - -1.7 3,631,663 - -3.2 3,631,665 + -3.7 3,631,665 + -0.1 3,631,672 + -2.6 3,631,672 - -2.3 3,631,710 + -1.4 3,631,738 - -0.6 3,631,767 + PGA1_c34610 0.12 +1.6 3,631,775 - PGA1_c34610 0.14 -0.1 3,631,775 - PGA1_c34610 0.14 +0.1 3,631,803 - PGA1_c34610 0.21 -0.4 3,631,855 - PGA1_c34610 0.34 +0.9 3,631,966 + PGA1_c34610 0.61 +1.4 3,631,974 - PGA1_c34610 0.63 +1.7 3,631,974 - PGA1_c34610 0.63 +0.0 3,631,974 - PGA1_c34610 0.63 +1.3 3,631,974 - PGA1_c34610 0.63 +2.2 3,632,035 - PGA1_c34610 0.79 +0.5 3,632,065 + PGA1_c34610 0.86 -1.9 3,632,112 + -1.3 3,632,172 - +0.4 3,632,183 - +1.0 3,632,221 + -0.0 3,632,229 - -0.1 3,632,229 - +0.8 3,632,260 + +1.4 3,632,285 - +1.7 3,632,290 + +0.1 3,632,290 - -0.9 3,632,319 - +1.8 3,632,321 + +0.4 3,632,329 - -0.1 3,632,329 - -0.7 3,632,350 - -0.6 3,632,396 + +0.3 3,632,402 - +1.1 3,632,417 + +0.4 3,632,419 + -0.1 3,632,419 + -0.3 3,632,419 + +0.6 3,632,425 - +0.4 3,632,427 - -0.3 3,632,443 + -0.1 3,632,451 - -0.5 3,632,453 + +0.1 3,632,453 + +0.4 3,632,453 + +0.4 3,632,453 + -0.1 3,632,453 + +0.7 3,632,453 + +0.4 3,632,453 + -1.1 3,632,453 + +0.5 3,632,453 + -0.3 3,632,453 + +1.1 3,632,456 - +0.5 3,632,461 - +1.0 3,632,461 - -0.1 3,632,461 - +1.4 3,632,461 - +0.0 3,632,461 - -0.1 3,632,461 - +2.8
Or see this region's nucleotide sequence