Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c34400 and PGA1_c34410 are separated by 65 nucleotides PGA1_c34410 and PGA1_c34420 are separated by 105 nucleotides PGA1_c34420 and PGA1_c34430 are separated by 114 nucleotides
PGA1_c34400: PGA1_c34400 - aminotransferase class-III, at 3,607,121 to 3,608,515
_c34400
PGA1_c34410: PGA1_c34410 - HTH-type transcriptional regulator, GntR family, at 3,608,581 to 3,609,303
_c34410
PGA1_c34420: PGA1_c34420 - putrescine-binding periplasmic protein PotF, at 3,609,409 to 3,610,494
_c34420
PGA1_c34430: PGA1_c34430 - putrescine transport ATP-binding protein PotG, at 3,610,609 to 3,611,736
_c34430
Position (kb)
3609
3610
3611 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3608.468 kb on - strand at 3608.615 kb on + strand at 3608.615 kb on + strand at 3608.615 kb on + strand at 3608.661 kb on - strand, within PGA1_c34410 at 3608.667 kb on - strand, within PGA1_c34410 at 3608.679 kb on - strand, within PGA1_c34410 at 3608.682 kb on - strand, within PGA1_c34410 at 3608.682 kb on - strand, within PGA1_c34410 at 3608.713 kb on + strand, within PGA1_c34410 at 3608.713 kb on + strand, within PGA1_c34410 at 3608.716 kb on - strand, within PGA1_c34410 at 3608.720 kb on + strand, within PGA1_c34410 at 3608.748 kb on + strand, within PGA1_c34410 at 3608.754 kb on + strand, within PGA1_c34410 at 3608.754 kb on + strand, within PGA1_c34410 at 3608.789 kb on + strand, within PGA1_c34410 at 3608.813 kb on + strand, within PGA1_c34410 at 3608.813 kb on + strand, within PGA1_c34410 at 3608.852 kb on - strand, within PGA1_c34410 at 3608.902 kb on - strand, within PGA1_c34410 at 3608.927 kb on + strand, within PGA1_c34410 at 3609.005 kb on + strand, within PGA1_c34410 at 3609.013 kb on - strand, within PGA1_c34410 at 3609.024 kb on - strand, within PGA1_c34410 at 3609.024 kb on - strand, within PGA1_c34410 at 3609.026 kb on + strand, within PGA1_c34410 at 3609.137 kb on + strand, within PGA1_c34410 at 3609.237 kb on + strand at 3609.309 kb on - strand at 3609.316 kb on + strand at 3609.345 kb on + strand at 3609.398 kb on + strand at 3609.413 kb on - strand at 3609.413 kb on - strand at 3609.453 kb on - strand at 3609.508 kb on + strand at 3609.545 kb on + strand, within PGA1_c34420 at 3609.550 kb on + strand, within PGA1_c34420 at 3609.550 kb on + strand, within PGA1_c34420 at 3609.550 kb on + strand, within PGA1_c34420 at 3609.558 kb on - strand, within PGA1_c34420 at 3609.558 kb on - strand, within PGA1_c34420 at 3609.558 kb on - strand, within PGA1_c34420 at 3609.575 kb on + strand, within PGA1_c34420 at 3609.583 kb on - strand, within PGA1_c34420 at 3609.692 kb on + strand, within PGA1_c34420 at 3609.712 kb on + strand, within PGA1_c34420 at 3609.712 kb on + strand, within PGA1_c34420 at 3609.716 kb on + strand, within PGA1_c34420 at 3609.719 kb on + strand, within PGA1_c34420 at 3609.802 kb on - strand, within PGA1_c34420 at 3609.802 kb on - strand, within PGA1_c34420 at 3609.815 kb on + strand, within PGA1_c34420 at 3609.829 kb on - strand, within PGA1_c34420 at 3609.830 kb on - strand, within PGA1_c34420 at 3609.860 kb on + strand, within PGA1_c34420 at 3609.860 kb on + strand, within PGA1_c34420 at 3609.868 kb on - strand, within PGA1_c34420 at 3609.973 kb on + strand, within PGA1_c34420 at 3610.000 kb on - strand, within PGA1_c34420 at 3610.002 kb on + strand, within PGA1_c34420 at 3610.002 kb on + strand, within PGA1_c34420 at 3610.002 kb on + strand, within PGA1_c34420 at 3610.008 kb on + strand, within PGA1_c34420 at 3610.008 kb on + strand, within PGA1_c34420 at 3610.010 kb on - strand, within PGA1_c34420 at 3610.010 kb on - strand, within PGA1_c34420 at 3610.010 kb on - strand, within PGA1_c34420 at 3610.010 kb on - strand, within PGA1_c34420 at 3610.010 kb on - strand, within PGA1_c34420 at 3610.015 kb on - strand, within PGA1_c34420 at 3610.015 kb on - strand, within PGA1_c34420 at 3610.020 kb on + strand, within PGA1_c34420 at 3610.058 kb on + strand, within PGA1_c34420 at 3610.076 kb on - strand, within PGA1_c34420 at 3610.095 kb on - strand, within PGA1_c34420 at 3610.115 kb on - strand, within PGA1_c34420 at 3610.125 kb on - strand, within PGA1_c34420 at 3610.150 kb on - strand, within PGA1_c34420 at 3610.150 kb on - strand, within PGA1_c34420 at 3610.303 kb on + strand, within PGA1_c34420 at 3610.388 kb on + strand at 3610.437 kb on + strand at 3610.444 kb on + strand at 3610.449 kb on + strand at 3610.449 kb on + strand at 3610.449 kb on + strand at 3610.450 kb on - strand at 3610.452 kb on - strand at 3610.457 kb on - strand at 3610.457 kb on - strand at 3610.457 kb on - strand at 3610.457 kb on - strand at 3610.457 kb on - strand at 3610.457 kb on - strand at 3610.476 kb on + strand at 3610.516 kb on + strand at 3610.526 kb on - strand at 3610.564 kb on + strand at 3610.601 kb on + strand at 3610.601 kb on + strand at 3610.609 kb on - strand at 3610.640 kb on + strand at 3610.695 kb on + strand at 3610.724 kb on + strand, within PGA1_c34430 at 3610.767 kb on - strand, within PGA1_c34430 at 3610.793 kb on + strand, within PGA1_c34430 at 3610.803 kb on + strand, within PGA1_c34430 at 3610.809 kb on - strand, within PGA1_c34430 at 3610.819 kb on - strand, within PGA1_c34430 at 3610.840 kb on + strand, within PGA1_c34430 at 3610.840 kb on + strand, within PGA1_c34430 at 3610.848 kb on - strand, within PGA1_c34430 at 3610.848 kb on - strand, within PGA1_c34430 at 3610.848 kb on - strand, within PGA1_c34430 at 3610.848 kb on - strand, within PGA1_c34430 at 3610.878 kb on + strand, within PGA1_c34430 at 3610.948 kb on - strand, within PGA1_c34430 at 3611.077 kb on + strand, within PGA1_c34430 at 3611.077 kb on + strand, within PGA1_c34430 at 3611.077 kb on + strand, within PGA1_c34430 at 3611.077 kb on + strand, within PGA1_c34430 at 3611.125 kb on + strand, within PGA1_c34430 at 3611.222 kb on + strand, within PGA1_c34430 at 3611.222 kb on + strand, within PGA1_c34430 at 3611.230 kb on - strand, within PGA1_c34430 at 3611.248 kb on + strand, within PGA1_c34430 at 3611.293 kb on + strand, within PGA1_c34430 at 3611.300 kb on + strand, within PGA1_c34430 at 3611.303 kb on - strand, within PGA1_c34430 at 3611.393 kb on + strand, within PGA1_c34430 at 3611.443 kb on + strand, within PGA1_c34430 at 3611.478 kb on - strand, within PGA1_c34430 at 3611.478 kb on - strand, within PGA1_c34430
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 3,608,468 - +0.8 3,608,615 + -0.4 3,608,615 + +2.0 3,608,615 + -1.2 3,608,661 - PGA1_c34410 0.11 -0.6 3,608,667 - PGA1_c34410 0.12 -0.1 3,608,679 - PGA1_c34410 0.14 +0.4 3,608,682 - PGA1_c34410 0.14 +0.8 3,608,682 - PGA1_c34410 0.14 +0.2 3,608,713 + PGA1_c34410 0.18 -0.7 3,608,713 + PGA1_c34410 0.18 +0.9 3,608,716 - PGA1_c34410 0.19 -1.1 3,608,720 + PGA1_c34410 0.19 +0.1 3,608,748 + PGA1_c34410 0.23 +0.9 3,608,754 + PGA1_c34410 0.24 +0.6 3,608,754 + PGA1_c34410 0.24 +0.9 3,608,789 + PGA1_c34410 0.29 +0.6 3,608,813 + PGA1_c34410 0.32 +2.2 3,608,813 + PGA1_c34410 0.32 +0.2 3,608,852 - PGA1_c34410 0.37 +1.3 3,608,902 - PGA1_c34410 0.44 +0.5 3,608,927 + PGA1_c34410 0.48 -0.4 3,609,005 + PGA1_c34410 0.59 +0.6 3,609,013 - PGA1_c34410 0.60 +0.6 3,609,024 - PGA1_c34410 0.61 +0.1 3,609,024 - PGA1_c34410 0.61 -1.4 3,609,026 + PGA1_c34410 0.62 +0.3 3,609,137 + PGA1_c34410 0.77 -0.8 3,609,237 + -0.1 3,609,309 - +0.6 3,609,316 + -1.1 3,609,345 + -0.4 3,609,398 + +0.0 3,609,413 - +0.7 3,609,413 - -1.4 3,609,453 - +1.7 3,609,508 + -0.4 3,609,545 + PGA1_c34420 0.13 -0.1 3,609,550 + PGA1_c34420 0.13 -1.7 3,609,550 + PGA1_c34420 0.13 -1.1 3,609,550 + PGA1_c34420 0.13 +0.2 3,609,558 - PGA1_c34420 0.14 +0.2 3,609,558 - PGA1_c34420 0.14 -1.6 3,609,558 - PGA1_c34420 0.14 -0.3 3,609,575 + PGA1_c34420 0.15 -1.0 3,609,583 - PGA1_c34420 0.16 -2.5 3,609,692 + PGA1_c34420 0.26 +0.3 3,609,712 + PGA1_c34420 0.28 -1.5 3,609,712 + PGA1_c34420 0.28 +1.4 3,609,716 + PGA1_c34420 0.28 +1.2 3,609,719 + PGA1_c34420 0.29 -0.1 3,609,802 - PGA1_c34420 0.36 -0.6 3,609,802 - PGA1_c34420 0.36 -1.1 3,609,815 + PGA1_c34420 0.37 -0.1 3,609,829 - PGA1_c34420 0.39 +0.3 3,609,830 - PGA1_c34420 0.39 -0.4 3,609,860 + PGA1_c34420 0.42 -0.7 3,609,860 + PGA1_c34420 0.42 +0.6 3,609,868 - PGA1_c34420 0.42 -0.7 3,609,973 + PGA1_c34420 0.52 +0.6 3,610,000 - PGA1_c34420 0.54 -1.7 3,610,002 + PGA1_c34420 0.55 +0.3 3,610,002 + PGA1_c34420 0.55 +0.3 3,610,002 + PGA1_c34420 0.55 -1.9 3,610,008 + PGA1_c34420 0.55 +0.0 3,610,008 + PGA1_c34420 0.55 -0.7 3,610,010 - PGA1_c34420 0.55 -1.6 3,610,010 - PGA1_c34420 0.55 -0.4 3,610,010 - PGA1_c34420 0.55 -0.9 3,610,010 - PGA1_c34420 0.55 +0.0 3,610,010 - PGA1_c34420 0.55 -2.0 3,610,015 - PGA1_c34420 0.56 -0.9 3,610,015 - PGA1_c34420 0.56 +1.4 3,610,020 + PGA1_c34420 0.56 +0.1 3,610,058 + PGA1_c34420 0.60 +0.3 3,610,076 - PGA1_c34420 0.61 +1.0 3,610,095 - PGA1_c34420 0.63 -0.1 3,610,115 - PGA1_c34420 0.65 +0.2 3,610,125 - PGA1_c34420 0.66 +1.4 3,610,150 - PGA1_c34420 0.68 +0.4 3,610,150 - PGA1_c34420 0.68 -0.6 3,610,303 + PGA1_c34420 0.82 -1.1 3,610,388 + +0.0 3,610,437 + -2.4 3,610,444 + -0.9 3,610,449 + -1.9 3,610,449 + +1.0 3,610,449 + -1.2 3,610,450 - +1.4 3,610,452 - +2.2 3,610,457 - -1.0 3,610,457 - +1.3 3,610,457 - -1.3 3,610,457 - -0.4 3,610,457 - -0.1 3,610,457 - +0.7 3,610,476 + +1.3 3,610,516 + +2.1 3,610,526 - +0.4 3,610,564 + -0.5 3,610,601 + -1.3 3,610,601 + +1.1 3,610,609 - -1.1 3,610,640 + -1.7 3,610,695 + -1.3 3,610,724 + PGA1_c34430 0.10 +2.1 3,610,767 - PGA1_c34430 0.14 +2.0 3,610,793 + PGA1_c34430 0.16 -1.9 3,610,803 + PGA1_c34430 0.17 -0.7 3,610,809 - PGA1_c34430 0.18 -0.5 3,610,819 - PGA1_c34430 0.19 -0.6 3,610,840 + PGA1_c34430 0.20 +0.4 3,610,840 + PGA1_c34430 0.20 +0.9 3,610,848 - PGA1_c34430 0.21 +1.4 3,610,848 - PGA1_c34430 0.21 -1.5 3,610,848 - PGA1_c34430 0.21 -1.4 3,610,848 - PGA1_c34430 0.21 +1.0 3,610,878 + PGA1_c34430 0.24 -1.2 3,610,948 - PGA1_c34430 0.30 +1.0 3,611,077 + PGA1_c34430 0.41 +0.4 3,611,077 + PGA1_c34430 0.41 -1.4 3,611,077 + PGA1_c34430 0.41 +0.4 3,611,077 + PGA1_c34430 0.41 -0.7 3,611,125 + PGA1_c34430 0.46 -0.5 3,611,222 + PGA1_c34430 0.54 +1.3 3,611,222 + PGA1_c34430 0.54 -2.0 3,611,230 - PGA1_c34430 0.55 -1.1 3,611,248 + PGA1_c34430 0.57 +3.0 3,611,293 + PGA1_c34430 0.61 +2.0 3,611,300 + PGA1_c34430 0.61 +0.2 3,611,303 - PGA1_c34430 0.62 +1.4 3,611,393 + PGA1_c34430 0.70 -0.3 3,611,443 + PGA1_c34430 0.74 +0.2 3,611,478 - PGA1_c34430 0.77 +1.1 3,611,478 - PGA1_c34430 0.77 -0.0
Or see this region's nucleotide sequence