Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c34310 and PGA1_c34320 are separated by 151 nucleotides PGA1_c34320 and PGA1_c34330 are separated by 14 nucleotides
PGA1_c34310: PGA1_c34310 - Uncharacterized Fe-S protein, at 3,596,415 to 3,597,467
_c34310
PGA1_c34320: PGA1_c34320 - L-threonine 3-dehydrogenase (EC 1.1.1.103) (from data) , at 3,597,619 to 3,598,647
_c34320
PGA1_c34330: PGA1_c34330 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data) , at 3,598,662 to 3,599,849
_c34330
Position (kb)
3597
3598
3599 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3596.641 kb on - strand, within PGA1_c34310 at 3596.905 kb on - strand, within PGA1_c34310 at 3596.905 kb on - strand, within PGA1_c34310 at 3596.993 kb on - strand, within PGA1_c34310 at 3597.008 kb on - strand, within PGA1_c34310 at 3597.038 kb on + strand, within PGA1_c34310 at 3597.052 kb on + strand, within PGA1_c34310 at 3597.063 kb on + strand, within PGA1_c34310 at 3597.066 kb on - strand, within PGA1_c34310 at 3597.066 kb on - strand, within PGA1_c34310 at 3597.071 kb on - strand, within PGA1_c34310 at 3597.140 kb on + strand, within PGA1_c34310 at 3597.140 kb on + strand, within PGA1_c34310 at 3597.140 kb on + strand, within PGA1_c34310 at 3597.140 kb on + strand, within PGA1_c34310 at 3597.148 kb on - strand, within PGA1_c34310 at 3597.148 kb on - strand, within PGA1_c34310 at 3597.148 kb on - strand, within PGA1_c34310 at 3597.148 kb on - strand, within PGA1_c34310 at 3597.148 kb on - strand, within PGA1_c34310 at 3597.211 kb on - strand, within PGA1_c34310 at 3597.265 kb on + strand, within PGA1_c34310 at 3597.313 kb on - strand, within PGA1_c34310 at 3597.313 kb on - strand, within PGA1_c34310 at 3597.313 kb on - strand, within PGA1_c34310 at 3597.377 kb on + strand at 3597.411 kb on + strand at 3597.418 kb on + strand at 3597.418 kb on + strand at 3597.465 kb on - strand at 3597.470 kb on + strand at 3597.521 kb on + strand at 3597.521 kb on - strand at 3597.543 kb on - strand at 3597.545 kb on + strand at 3597.568 kb on + strand at 3597.593 kb on + strand at 3597.593 kb on + strand at 3597.618 kb on + strand at 3597.626 kb on - strand at 3597.639 kb on - strand at 3597.715 kb on - strand at 3597.731 kb on - strand, within PGA1_c34320 at 3597.731 kb on - strand, within PGA1_c34320 at 3597.761 kb on - strand, within PGA1_c34320 at 3597.816 kb on - strand, within PGA1_c34320 at 3597.857 kb on - strand, within PGA1_c34320 at 3597.878 kb on + strand, within PGA1_c34320 at 3597.886 kb on - strand, within PGA1_c34320 at 3597.938 kb on - strand, within PGA1_c34320 at 3597.979 kb on + strand, within PGA1_c34320 at 3597.996 kb on - strand, within PGA1_c34320 at 3598.034 kb on - strand, within PGA1_c34320 at 3598.055 kb on + strand, within PGA1_c34320 at 3598.128 kb on + strand, within PGA1_c34320 at 3598.128 kb on + strand, within PGA1_c34320 at 3598.136 kb on + strand, within PGA1_c34320 at 3598.136 kb on + strand, within PGA1_c34320 at 3598.172 kb on - strand, within PGA1_c34320 at 3598.179 kb on - strand, within PGA1_c34320 at 3598.179 kb on - strand, within PGA1_c34320 at 3598.190 kb on + strand, within PGA1_c34320 at 3598.198 kb on - strand, within PGA1_c34320 at 3598.198 kb on - strand, within PGA1_c34320 at 3598.255 kb on + strand, within PGA1_c34320 at 3598.269 kb on - strand, within PGA1_c34320 at 3598.354 kb on - strand, within PGA1_c34320 at 3598.365 kb on - strand, within PGA1_c34320 at 3598.386 kb on + strand, within PGA1_c34320 at 3598.394 kb on - strand, within PGA1_c34320 at 3598.394 kb on - strand, within PGA1_c34320 at 3598.394 kb on - strand, within PGA1_c34320 at 3598.396 kb on + strand, within PGA1_c34320 at 3598.399 kb on - strand, within PGA1_c34320 at 3598.404 kb on - strand, within PGA1_c34320 at 3598.489 kb on - strand, within PGA1_c34320 at 3598.500 kb on + strand, within PGA1_c34320 at 3598.508 kb on - strand, within PGA1_c34320 at 3598.566 kb on - strand at 3598.566 kb on - strand at 3598.577 kb on - strand at 3598.614 kb on - strand at 3598.660 kb on + strand at 3598.660 kb on + strand at 3598.668 kb on - strand at 3598.761 kb on - strand at 3598.842 kb on - strand, within PGA1_c34330 at 3598.842 kb on - strand, within PGA1_c34330 at 3598.877 kb on - strand, within PGA1_c34330 at 3598.917 kb on + strand, within PGA1_c34330 at 3598.917 kb on + strand, within PGA1_c34330 at 3598.925 kb on - strand, within PGA1_c34330 at 3599.117 kb on + strand, within PGA1_c34330 at 3599.130 kb on + strand, within PGA1_c34330 at 3599.170 kb on - strand, within PGA1_c34330 at 3599.348 kb on - strand, within PGA1_c34330 at 3599.393 kb on + strand, within PGA1_c34330 at 3599.398 kb on + strand, within PGA1_c34330 at 3599.401 kb on - strand, within PGA1_c34330 at 3599.410 kb on + strand, within PGA1_c34330 at 3599.410 kb on + strand, within PGA1_c34330 at 3599.418 kb on - strand, within PGA1_c34330 at 3599.420 kb on + strand, within PGA1_c34330 at 3599.428 kb on - strand, within PGA1_c34330 at 3599.428 kb on - strand, within PGA1_c34330 at 3599.455 kb on - strand, within PGA1_c34330 at 3599.479 kb on + strand, within PGA1_c34330 at 3599.487 kb on - strand, within PGA1_c34330 at 3599.616 kb on + strand, within PGA1_c34330 at 3599.628 kb on + strand, within PGA1_c34330
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 3,596,641 - PGA1_c34310 0.21 +0.9 3,596,905 - PGA1_c34310 0.47 +0.1 3,596,905 - PGA1_c34310 0.47 -0.9 3,596,993 - PGA1_c34310 0.55 -1.3 3,597,008 - PGA1_c34310 0.56 -2.3 3,597,038 + PGA1_c34310 0.59 +0.0 3,597,052 + PGA1_c34310 0.60 +0.9 3,597,063 + PGA1_c34310 0.62 +0.2 3,597,066 - PGA1_c34310 0.62 -1.4 3,597,066 - PGA1_c34310 0.62 -0.6 3,597,071 - PGA1_c34310 0.62 -0.5 3,597,140 + PGA1_c34310 0.69 -2.0 3,597,140 + PGA1_c34310 0.69 +0.1 3,597,140 + PGA1_c34310 0.69 -0.4 3,597,140 + PGA1_c34310 0.69 +1.0 3,597,148 - PGA1_c34310 0.70 -0.4 3,597,148 - PGA1_c34310 0.70 -0.7 3,597,148 - PGA1_c34310 0.70 +0.2 3,597,148 - PGA1_c34310 0.70 -2.1 3,597,148 - PGA1_c34310 0.70 +1.4 3,597,211 - PGA1_c34310 0.76 -0.1 3,597,265 + PGA1_c34310 0.81 -1.6 3,597,313 - PGA1_c34310 0.85 -0.1 3,597,313 - PGA1_c34310 0.85 +0.7 3,597,313 - PGA1_c34310 0.85 -0.2 3,597,377 + +0.6 3,597,411 + +0.3 3,597,418 + +1.4 3,597,418 + +2.2 3,597,465 - +0.3 3,597,470 + -1.1 3,597,521 + -0.8 3,597,521 - +2.0 3,597,543 - +1.9 3,597,545 + +0.0 3,597,568 + -1.0 3,597,593 + -0.9 3,597,593 + +1.2 3,597,618 + +0.1 3,597,626 - -1.6 3,597,639 - -2.5 3,597,715 - -1.4 3,597,731 - PGA1_c34320 0.11 -3.5 3,597,731 - PGA1_c34320 0.11 -1.4 3,597,761 - PGA1_c34320 0.14 -1.1 3,597,816 - PGA1_c34320 0.19 -0.1 3,597,857 - PGA1_c34320 0.23 -2.8 3,597,878 + PGA1_c34320 0.25 -1.9 3,597,886 - PGA1_c34320 0.26 -1.6 3,597,938 - PGA1_c34320 0.31 +0.4 3,597,979 + PGA1_c34320 0.35 -3.0 3,597,996 - PGA1_c34320 0.37 -1.6 3,598,034 - PGA1_c34320 0.40 -0.1 3,598,055 + PGA1_c34320 0.42 -2.3 3,598,128 + PGA1_c34320 0.49 -2.6 3,598,128 + PGA1_c34320 0.49 -1.9 3,598,136 + PGA1_c34320 0.50 -0.6 3,598,136 + PGA1_c34320 0.50 -1.4 3,598,172 - PGA1_c34320 0.54 -1.9 3,598,179 - PGA1_c34320 0.54 -1.1 3,598,179 - PGA1_c34320 0.54 -1.0 3,598,190 + PGA1_c34320 0.55 -3.3 3,598,198 - PGA1_c34320 0.56 -2.0 3,598,198 - PGA1_c34320 0.56 -1.7 3,598,255 + PGA1_c34320 0.62 -2.7 3,598,269 - PGA1_c34320 0.63 -2.3 3,598,354 - PGA1_c34320 0.71 -1.9 3,598,365 - PGA1_c34320 0.72 -1.7 3,598,386 + PGA1_c34320 0.75 -3.0 3,598,394 - PGA1_c34320 0.75 -2.8 3,598,394 - PGA1_c34320 0.75 -1.6 3,598,394 - PGA1_c34320 0.75 -1.7 3,598,396 + PGA1_c34320 0.76 -0.6 3,598,399 - PGA1_c34320 0.76 -1.9 3,598,404 - PGA1_c34320 0.76 -2.8 3,598,489 - PGA1_c34320 0.85 -2.8 3,598,500 + PGA1_c34320 0.86 -2.6 3,598,508 - PGA1_c34320 0.86 -1.1 3,598,566 - -3.4 3,598,566 - -0.1 3,598,577 - -1.9 3,598,614 - -1.1 3,598,660 + -0.1 3,598,660 + -2.0 3,598,668 - -1.9 3,598,761 - -0.6 3,598,842 - PGA1_c34330 0.15 -1.4 3,598,842 - PGA1_c34330 0.15 -1.1 3,598,877 - PGA1_c34330 0.18 -2.8 3,598,917 + PGA1_c34330 0.21 -1.1 3,598,917 + PGA1_c34330 0.21 -1.6 3,598,925 - PGA1_c34330 0.22 -2.0 3,599,117 + PGA1_c34330 0.38 -1.3 3,599,130 + PGA1_c34330 0.39 -3.4 3,599,170 - PGA1_c34330 0.43 -2.6 3,599,348 - PGA1_c34330 0.58 -0.1 3,599,393 + PGA1_c34330 0.62 -1.6 3,599,398 + PGA1_c34330 0.62 -1.6 3,599,401 - PGA1_c34330 0.62 -1.9 3,599,410 + PGA1_c34330 0.63 -1.9 3,599,410 + PGA1_c34330 0.63 -0.1 3,599,418 - PGA1_c34330 0.64 -2.8 3,599,420 + PGA1_c34330 0.64 -1.9 3,599,428 - PGA1_c34330 0.64 -1.6 3,599,428 - PGA1_c34330 0.64 -2.6 3,599,455 - PGA1_c34330 0.67 -2.1 3,599,479 + PGA1_c34330 0.69 -3.3 3,599,487 - PGA1_c34330 0.69 -0.6 3,599,616 + PGA1_c34330 0.80 -1.4 3,599,628 + PGA1_c34330 0.81 -1.1
Or see this region's nucleotide sequence