Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c32280 and PGA1_c32290 are separated by 116 nucleotides PGA1_c32290 and PGA1_c32300 overlap by 4 nucleotides
PGA1_c32280: PGA1_c32280 - Uncharacterized protein conserved in bacteria, at 3,385,347 to 3,386,291
_c32280
PGA1_c32290: PGA1_c32290 - HTH-type transcriptional regulator, IclR family, at 3,386,408 to 3,387,217
_c32290
PGA1_c32300: PGA1_c32300 - aminotransferase class-III, at 3,387,214 to 3,388,560
_c32300
Position (kb)
3386
3387
3388 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3385.429 kb on + strand at 3385.484 kb on + strand, within PGA1_c32280 at 3385.524 kb on - strand, within PGA1_c32280 at 3385.524 kb on - strand, within PGA1_c32280 at 3385.576 kb on - strand, within PGA1_c32280 at 3385.594 kb on - strand, within PGA1_c32280 at 3385.625 kb on + strand, within PGA1_c32280 at 3385.625 kb on + strand, within PGA1_c32280 at 3385.666 kb on - strand, within PGA1_c32280 at 3385.716 kb on + strand, within PGA1_c32280 at 3385.731 kb on - strand, within PGA1_c32280 at 3385.812 kb on - strand, within PGA1_c32280 at 3385.892 kb on + strand, within PGA1_c32280 at 3385.931 kb on - strand, within PGA1_c32280 at 3385.960 kb on - strand, within PGA1_c32280 at 3385.965 kb on - strand, within PGA1_c32280 at 3386.049 kb on - strand, within PGA1_c32280 at 3386.065 kb on + strand, within PGA1_c32280 at 3386.159 kb on - strand, within PGA1_c32280 at 3386.159 kb on - strand, within PGA1_c32280 at 3386.163 kb on + strand, within PGA1_c32280 at 3386.235 kb on + strand at 3386.411 kb on - strand at 3386.415 kb on - strand at 3386.446 kb on + strand at 3386.454 kb on - strand at 3386.454 kb on - strand at 3386.454 kb on - strand at 3386.480 kb on + strand at 3386.482 kb on + strand at 3386.490 kb on + strand, within PGA1_c32290 at 3386.493 kb on - strand, within PGA1_c32290 at 3386.617 kb on + strand, within PGA1_c32290 at 3386.638 kb on + strand, within PGA1_c32290 at 3386.646 kb on - strand, within PGA1_c32290 at 3386.704 kb on - strand, within PGA1_c32290 at 3386.763 kb on + strand, within PGA1_c32290 at 3386.819 kb on - strand, within PGA1_c32290 at 3386.838 kb on + strand, within PGA1_c32290 at 3386.851 kb on + strand, within PGA1_c32290 at 3386.926 kb on + strand, within PGA1_c32290 at 3386.958 kb on + strand, within PGA1_c32290 at 3386.958 kb on + strand, within PGA1_c32290 at 3386.963 kb on + strand, within PGA1_c32290 at 3387.004 kb on + strand, within PGA1_c32290 at 3387.078 kb on - strand, within PGA1_c32290 at 3387.093 kb on + strand, within PGA1_c32290 at 3387.098 kb on + strand, within PGA1_c32290 at 3387.103 kb on + strand, within PGA1_c32290 at 3387.103 kb on + strand, within PGA1_c32290 at 3387.111 kb on - strand, within PGA1_c32290 at 3387.111 kb on - strand, within PGA1_c32290 at 3387.111 kb on - strand, within PGA1_c32290 at 3387.111 kb on - strand, within PGA1_c32290 at 3387.157 kb on + strand at 3387.165 kb on - strand at 3387.175 kb on - strand at 3387.250 kb on + strand at 3387.272 kb on - strand at 3387.277 kb on + strand at 3387.282 kb on + strand at 3387.282 kb on + strand at 3387.285 kb on - strand at 3387.290 kb on - strand at 3387.295 kb on - strand at 3387.295 kb on - strand at 3387.307 kb on + strand at 3387.308 kb on - strand at 3387.350 kb on + strand, within PGA1_c32300 at 3387.368 kb on + strand, within PGA1_c32300 at 3387.376 kb on - strand, within PGA1_c32300 at 3387.401 kb on + strand, within PGA1_c32300 at 3387.423 kb on + strand, within PGA1_c32300 at 3387.450 kb on + strand, within PGA1_c32300 at 3387.464 kb on - strand, within PGA1_c32300 at 3387.511 kb on - strand, within PGA1_c32300 at 3387.511 kb on - strand, within PGA1_c32300 at 3387.529 kb on - strand, within PGA1_c32300 at 3387.530 kb on - strand, within PGA1_c32300 at 3387.532 kb on + strand, within PGA1_c32300 at 3387.576 kb on + strand, within PGA1_c32300 at 3387.608 kb on + strand, within PGA1_c32300 at 3387.670 kb on - strand, within PGA1_c32300 at 3387.738 kb on - strand, within PGA1_c32300 at 3387.763 kb on + strand, within PGA1_c32300 at 3387.763 kb on + strand, within PGA1_c32300 at 3387.763 kb on - strand, within PGA1_c32300 at 3387.764 kb on + strand, within PGA1_c32300 at 3387.770 kb on - strand, within PGA1_c32300 at 3387.771 kb on - strand, within PGA1_c32300 at 3387.771 kb on - strand, within PGA1_c32300 at 3387.811 kb on + strand, within PGA1_c32300 at 3387.816 kb on + strand, within PGA1_c32300 at 3387.819 kb on - strand, within PGA1_c32300 at 3387.837 kb on - strand, within PGA1_c32300 at 3387.908 kb on + strand, within PGA1_c32300 at 3387.951 kb on - strand, within PGA1_c32300 at 3387.968 kb on + strand, within PGA1_c32300 at 3387.975 kb on + strand, within PGA1_c32300 at 3388.017 kb on + strand, within PGA1_c32300 at 3388.017 kb on + strand, within PGA1_c32300 at 3388.025 kb on - strand, within PGA1_c32300 at 3388.043 kb on - strand, within PGA1_c32300 at 3388.054 kb on + strand, within PGA1_c32300 at 3388.054 kb on + strand, within PGA1_c32300 at 3388.054 kb on + strand, within PGA1_c32300 at 3388.054 kb on + strand, within PGA1_c32300 at 3388.067 kb on - strand, within PGA1_c32300 at 3388.116 kb on - strand, within PGA1_c32300 at 3388.143 kb on + strand, within PGA1_c32300 at 3388.157 kb on - strand, within PGA1_c32300 at 3388.172 kb on + strand, within PGA1_c32300 at 3388.178 kb on - strand, within PGA1_c32300
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 3,385,429 + -0.2 3,385,484 + PGA1_c32280 0.14 +1.6 3,385,524 - PGA1_c32280 0.19 -0.7 3,385,524 - PGA1_c32280 0.19 +1.6 3,385,576 - PGA1_c32280 0.24 +0.0 3,385,594 - PGA1_c32280 0.26 -0.3 3,385,625 + PGA1_c32280 0.29 -0.4 3,385,625 + PGA1_c32280 0.29 -1.5 3,385,666 - PGA1_c32280 0.34 +0.5 3,385,716 + PGA1_c32280 0.39 -0.1 3,385,731 - PGA1_c32280 0.41 +0.0 3,385,812 - PGA1_c32280 0.49 +1.9 3,385,892 + PGA1_c32280 0.58 -1.5 3,385,931 - PGA1_c32280 0.62 -0.3 3,385,960 - PGA1_c32280 0.65 -0.5 3,385,965 - PGA1_c32280 0.65 +3.6 3,386,049 - PGA1_c32280 0.74 -0.5 3,386,065 + PGA1_c32280 0.76 +0.5 3,386,159 - PGA1_c32280 0.86 +1.3 3,386,159 - PGA1_c32280 0.86 +1.6 3,386,163 + PGA1_c32280 0.86 -1.2 3,386,235 + -1.8 3,386,411 - -1.0 3,386,415 - +1.3 3,386,446 + +0.7 3,386,454 - -0.7 3,386,454 - -0.3 3,386,454 - -1.3 3,386,480 + +2.7 3,386,482 + -0.5 3,386,490 + PGA1_c32290 0.10 +0.8 3,386,493 - PGA1_c32290 0.10 -1.3 3,386,617 + PGA1_c32290 0.26 -0.5 3,386,638 + PGA1_c32290 0.28 -2.5 3,386,646 - PGA1_c32290 0.29 -0.5 3,386,704 - PGA1_c32290 0.37 +0.8 3,386,763 + PGA1_c32290 0.44 -0.7 3,386,819 - PGA1_c32290 0.51 -0.3 3,386,838 + PGA1_c32290 0.53 +0.7 3,386,851 + PGA1_c32290 0.55 -1.0 3,386,926 + PGA1_c32290 0.64 -0.1 3,386,958 + PGA1_c32290 0.68 +1.0 3,386,958 + PGA1_c32290 0.68 +0.0 3,386,963 + PGA1_c32290 0.69 -0.5 3,387,004 + PGA1_c32290 0.74 -1.0 3,387,078 - PGA1_c32290 0.83 -0.8 3,387,093 + PGA1_c32290 0.85 -1.0 3,387,098 + PGA1_c32290 0.85 -0.8 3,387,103 + PGA1_c32290 0.86 -1.8 3,387,103 + PGA1_c32290 0.86 -1.4 3,387,111 - PGA1_c32290 0.87 +0.3 3,387,111 - PGA1_c32290 0.87 -0.3 3,387,111 - PGA1_c32290 0.87 -0.7 3,387,111 - PGA1_c32290 0.87 -1.0 3,387,157 + -0.4 3,387,165 - +1.9 3,387,175 - -0.7 3,387,250 + +1.0 3,387,272 - -0.3 3,387,277 + +1.0 3,387,282 + +1.5 3,387,282 + +0.8 3,387,285 - -0.3 3,387,290 - -0.5 3,387,295 - -0.5 3,387,295 - +1.6 3,387,307 + +1.8 3,387,308 - +0.3 3,387,350 + PGA1_c32300 0.10 -0.3 3,387,368 + PGA1_c32300 0.11 -1.8 3,387,376 - PGA1_c32300 0.12 +1.3 3,387,401 + PGA1_c32300 0.14 +0.3 3,387,423 + PGA1_c32300 0.16 -0.5 3,387,450 + PGA1_c32300 0.18 +0.3 3,387,464 - PGA1_c32300 0.19 -0.8 3,387,511 - PGA1_c32300 0.22 +1.1 3,387,511 - PGA1_c32300 0.22 -1.8 3,387,529 - PGA1_c32300 0.23 +0.0 3,387,530 - PGA1_c32300 0.23 +0.5 3,387,532 + PGA1_c32300 0.24 +0.2 3,387,576 + PGA1_c32300 0.27 +1.8 3,387,608 + PGA1_c32300 0.29 -1.1 3,387,670 - PGA1_c32300 0.34 -1.0 3,387,738 - PGA1_c32300 0.39 -0.4 3,387,763 + PGA1_c32300 0.41 +0.5 3,387,763 + PGA1_c32300 0.41 +0.3 3,387,763 - PGA1_c32300 0.41 -1.1 3,387,764 + PGA1_c32300 0.41 -0.5 3,387,770 - PGA1_c32300 0.41 +0.6 3,387,771 - PGA1_c32300 0.41 +1.3 3,387,771 - PGA1_c32300 0.41 -1.7 3,387,811 + PGA1_c32300 0.44 -1.3 3,387,816 + PGA1_c32300 0.45 -0.1 3,387,819 - PGA1_c32300 0.45 -0.7 3,387,837 - PGA1_c32300 0.46 -0.6 3,387,908 + PGA1_c32300 0.52 -0.3 3,387,951 - PGA1_c32300 0.55 -0.1 3,387,968 + PGA1_c32300 0.56 +0.7 3,387,975 + PGA1_c32300 0.56 +1.3 3,388,017 + PGA1_c32300 0.60 +0.2 3,388,017 + PGA1_c32300 0.60 -2.4 3,388,025 - PGA1_c32300 0.60 -1.3 3,388,043 - PGA1_c32300 0.62 -0.1 3,388,054 + PGA1_c32300 0.62 +0.7 3,388,054 + PGA1_c32300 0.62 -2.2 3,388,054 + PGA1_c32300 0.62 -2.3 3,388,054 + PGA1_c32300 0.62 -0.5 3,388,067 - PGA1_c32300 0.63 +0.5 3,388,116 - PGA1_c32300 0.67 +0.1 3,388,143 + PGA1_c32300 0.69 +1.3 3,388,157 - PGA1_c32300 0.70 -1.7 3,388,172 + PGA1_c32300 0.71 +1.0 3,388,178 - PGA1_c32300 0.72 -0.7
Or see this region's nucleotide sequence