Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c28870 and PGA1_c28880 overlap by 19 nucleotides PGA1_c28880 and PGA1_c28890 overlap by 4 nucleotides PGA1_c28890 and PGA1_c28900 overlap by 1 nucleotides
PGA1_c28870: PGA1_c28870 - putative molybdopterin-containing oxidoreductase, at 3,027,564 to 3,030,404
_c28870
PGA1_c28880: PGA1_c28880 - hypothetical protein, at 3,030,386 to 3,030,967
_c28880
PGA1_c28890: PGA1_c28890 - polysulfide reductase chain B-like protein, at 3,030,964 to 3,031,758
_c28890
PGA1_c28900: PGA1_c28900 - putative DMSO reductase anchor subunit, at 3,031,758 to 3,032,645
_c28900
Position (kb)
3030
3031 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3029.405 kb on + strand, within PGA1_c28870 at 3029.413 kb on - strand, within PGA1_c28870 at 3029.445 kb on + strand, within PGA1_c28870 at 3029.531 kb on + strand, within PGA1_c28870 at 3029.539 kb on - strand, within PGA1_c28870 at 3029.539 kb on - strand, within PGA1_c28870 at 3029.546 kb on + strand, within PGA1_c28870 at 3029.562 kb on - strand, within PGA1_c28870 at 3029.564 kb on + strand, within PGA1_c28870 at 3029.570 kb on + strand, within PGA1_c28870 at 3029.575 kb on + strand, within PGA1_c28870 at 3029.575 kb on + strand, within PGA1_c28870 at 3029.622 kb on + strand, within PGA1_c28870 at 3029.627 kb on + strand, within PGA1_c28870 at 3029.627 kb on + strand, within PGA1_c28870 at 3029.629 kb on + strand, within PGA1_c28870 at 3029.688 kb on - strand, within PGA1_c28870 at 3029.698 kb on - strand, within PGA1_c28870 at 3029.705 kb on - strand, within PGA1_c28870 at 3029.705 kb on - strand, within PGA1_c28870 at 3029.707 kb on + strand, within PGA1_c28870 at 3029.709 kb on + strand at 3029.715 kb on - strand, within PGA1_c28870 at 3029.715 kb on - strand, within PGA1_c28870 at 3029.715 kb on - strand, within PGA1_c28870 at 3029.722 kb on - strand, within PGA1_c28870 at 3029.722 kb on - strand, within PGA1_c28870 at 3029.729 kb on + strand, within PGA1_c28870 at 3029.736 kb on + strand, within PGA1_c28870 at 3029.742 kb on - strand, within PGA1_c28870 at 3029.783 kb on + strand, within PGA1_c28870 at 3029.807 kb on - strand, within PGA1_c28870 at 3029.897 kb on - strand, within PGA1_c28870 at 3029.898 kb on - strand, within PGA1_c28870 at 3029.898 kb on - strand, within PGA1_c28870 at 3029.921 kb on + strand, within PGA1_c28870 at 3029.923 kb on - strand, within PGA1_c28870 at 3029.929 kb on - strand, within PGA1_c28870 at 3029.962 kb on + strand, within PGA1_c28870 at 3030.099 kb on + strand, within PGA1_c28870 at 3030.107 kb on - strand, within PGA1_c28870 at 3030.125 kb on + strand at 3030.144 kb on - strand at 3030.165 kb on - strand at 3030.167 kb on + strand at 3030.226 kb on + strand at 3030.344 kb on + strand at 3030.345 kb on + strand at 3030.423 kb on + strand at 3030.473 kb on + strand, within PGA1_c28880 at 3030.492 kb on - strand, within PGA1_c28880 at 3030.515 kb on + strand, within PGA1_c28880 at 3030.515 kb on + strand, within PGA1_c28880 at 3030.520 kb on + strand, within PGA1_c28880 at 3030.524 kb on + strand, within PGA1_c28880 at 3030.618 kb on + strand, within PGA1_c28880 at 3030.618 kb on + strand, within PGA1_c28880 at 3030.658 kb on + strand, within PGA1_c28880 at 3030.673 kb on + strand, within PGA1_c28880 at 3030.673 kb on + strand, within PGA1_c28880 at 3030.707 kb on + strand, within PGA1_c28880 at 3030.719 kb on + strand, within PGA1_c28880 at 3030.726 kb on - strand, within PGA1_c28880 at 3030.750 kb on + strand, within PGA1_c28880 at 3030.770 kb on - strand, within PGA1_c28880 at 3030.795 kb on + strand, within PGA1_c28880 at 3030.803 kb on - strand, within PGA1_c28880 at 3030.842 kb on + strand, within PGA1_c28880 at 3030.874 kb on + strand, within PGA1_c28880 at 3030.889 kb on + strand, within PGA1_c28880 at 3030.889 kb on + strand, within PGA1_c28880 at 3030.889 kb on + strand, within PGA1_c28880 at 3030.889 kb on + strand, within PGA1_c28880 at 3030.897 kb on - strand, within PGA1_c28880 at 3030.897 kb on - strand, within PGA1_c28880 at 3030.986 kb on - strand at 3030.986 kb on - strand at 3030.986 kb on - strand at 3031.010 kb on - strand at 3031.060 kb on + strand, within PGA1_c28890 at 3031.060 kb on + strand, within PGA1_c28890 at 3031.060 kb on + strand, within PGA1_c28890 at 3031.060 kb on + strand, within PGA1_c28890 at 3031.068 kb on - strand, within PGA1_c28890 at 3031.097 kb on - strand, within PGA1_c28890 at 3031.130 kb on - strand, within PGA1_c28890 at 3031.134 kb on + strand, within PGA1_c28890 at 3031.167 kb on + strand, within PGA1_c28890 at 3031.183 kb on - strand, within PGA1_c28890 at 3031.185 kb on + strand, within PGA1_c28890 at 3031.193 kb on - strand, within PGA1_c28890 at 3031.295 kb on - strand, within PGA1_c28890 at 3031.298 kb on + strand, within PGA1_c28890 at 3031.306 kb on - strand, within PGA1_c28890 at 3031.331 kb on + strand, within PGA1_c28890 at 3031.331 kb on + strand, within PGA1_c28890 at 3031.331 kb on + strand, within PGA1_c28890 at 3031.331 kb on + strand, within PGA1_c28890 at 3031.339 kb on - strand, within PGA1_c28890 at 3031.339 kb on - strand, within PGA1_c28890 at 3031.350 kb on + strand, within PGA1_c28890 at 3031.415 kb on + strand, within PGA1_c28890 at 3031.423 kb on - strand, within PGA1_c28890 at 3031.428 kb on - strand, within PGA1_c28890 at 3031.467 kb on - strand, within PGA1_c28890 at 3031.489 kb on - strand, within PGA1_c28890 at 3031.559 kb on - strand, within PGA1_c28890 at 3031.703 kb on + strand at 3031.739 kb on + strand at 3031.774 kb on + strand at 3031.786 kb on + strand at 3031.793 kb on + strand at 3031.807 kb on + strand at 3031.815 kb on - strand at 3031.815 kb on - strand at 3031.815 kb on - strand at 3031.828 kb on - strand at 3031.856 kb on - strand, within PGA1_c28900 at 3031.856 kb on - strand, within PGA1_c28900 at 3031.858 kb on - strand, within PGA1_c28900 at 3031.871 kb on + strand, within PGA1_c28900 at 3031.871 kb on - strand, within PGA1_c28900 at 3031.899 kb on - strand, within PGA1_c28900 at 3031.921 kb on + strand, within PGA1_c28900 at 3031.941 kb on + strand, within PGA1_c28900 at 3031.949 kb on - strand, within PGA1_c28900 at 3031.954 kb on - strand, within PGA1_c28900 at 3031.957 kb on + strand, within PGA1_c28900
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 3,029,405 + PGA1_c28870 0.65 -0.2 3,029,413 - PGA1_c28870 0.65 -1.3 3,029,445 + PGA1_c28870 0.66 +1.5 3,029,531 + PGA1_c28870 0.69 -0.6 3,029,539 - PGA1_c28870 0.70 -2.3 3,029,539 - PGA1_c28870 0.70 +0.1 3,029,546 + PGA1_c28870 0.70 -0.8 3,029,562 - PGA1_c28870 0.70 -0.3 3,029,564 + PGA1_c28870 0.70 -0.8 3,029,570 + PGA1_c28870 0.71 +0.8 3,029,575 + PGA1_c28870 0.71 +1.1 3,029,575 + PGA1_c28870 0.71 -0.8 3,029,622 + PGA1_c28870 0.72 +0.1 3,029,627 + PGA1_c28870 0.73 +0.5 3,029,627 + PGA1_c28870 0.73 +1.0 3,029,629 + PGA1_c28870 0.73 -0.8 3,029,688 - PGA1_c28870 0.75 +0.5 3,029,698 - PGA1_c28870 0.75 -0.1 3,029,705 - PGA1_c28870 0.75 -0.2 3,029,705 - PGA1_c28870 0.75 -0.8 3,029,707 + PGA1_c28870 0.75 +0.7 3,029,709 + +0.7 3,029,715 - PGA1_c28870 0.76 -0.7 3,029,715 - PGA1_c28870 0.76 -1.3 3,029,715 - PGA1_c28870 0.76 +0.7 3,029,722 - PGA1_c28870 0.76 -0.7 3,029,722 - PGA1_c28870 0.76 -1.1 3,029,729 + PGA1_c28870 0.76 -0.0 3,029,736 + PGA1_c28870 0.76 +0.9 3,029,742 - PGA1_c28870 0.77 +0.7 3,029,783 + PGA1_c28870 0.78 -1.2 3,029,807 - PGA1_c28870 0.79 +2.2 3,029,897 - PGA1_c28870 0.82 +0.9 3,029,898 - PGA1_c28870 0.82 +0.5 3,029,898 - PGA1_c28870 0.82 +1.4 3,029,921 + PGA1_c28870 0.83 +0.4 3,029,923 - PGA1_c28870 0.83 -1.1 3,029,929 - PGA1_c28870 0.83 -0.9 3,029,962 + PGA1_c28870 0.84 -1.2 3,030,099 + PGA1_c28870 0.89 +0.1 3,030,107 - PGA1_c28870 0.90 +0.2 3,030,125 + -0.8 3,030,144 - -0.1 3,030,165 - -0.4 3,030,167 + +0.2 3,030,226 + -0.3 3,030,344 + -1.0 3,030,345 + -0.7 3,030,423 + +0.4 3,030,473 + PGA1_c28880 0.15 -0.8 3,030,492 - PGA1_c28880 0.18 -0.6 3,030,515 + PGA1_c28880 0.22 -0.3 3,030,515 + PGA1_c28880 0.22 -0.7 3,030,520 + PGA1_c28880 0.23 -3.1 3,030,524 + PGA1_c28880 0.24 -1.0 3,030,618 + PGA1_c28880 0.40 +0.6 3,030,618 + PGA1_c28880 0.40 -1.9 3,030,658 + PGA1_c28880 0.47 +0.2 3,030,673 + PGA1_c28880 0.49 +1.0 3,030,673 + PGA1_c28880 0.49 +0.4 3,030,707 + PGA1_c28880 0.55 +0.2 3,030,719 + PGA1_c28880 0.57 +0.4 3,030,726 - PGA1_c28880 0.58 +1.2 3,030,750 + PGA1_c28880 0.63 -0.3 3,030,770 - PGA1_c28880 0.66 -1.2 3,030,795 + PGA1_c28880 0.70 +0.2 3,030,803 - PGA1_c28880 0.72 -0.1 3,030,842 + PGA1_c28880 0.78 +0.4 3,030,874 + PGA1_c28880 0.84 -0.9 3,030,889 + PGA1_c28880 0.86 -0.7 3,030,889 + PGA1_c28880 0.86 -0.8 3,030,889 + PGA1_c28880 0.86 -1.2 3,030,889 + PGA1_c28880 0.86 -0.3 3,030,897 - PGA1_c28880 0.88 +0.5 3,030,897 - PGA1_c28880 0.88 -1.1 3,030,986 - -0.3 3,030,986 - +0.7 3,030,986 - -0.5 3,031,010 - +0.8 3,031,060 + PGA1_c28890 0.12 +1.2 3,031,060 + PGA1_c28890 0.12 -0.1 3,031,060 + PGA1_c28890 0.12 -0.6 3,031,060 + PGA1_c28890 0.12 -0.8 3,031,068 - PGA1_c28890 0.13 +1.2 3,031,097 - PGA1_c28890 0.17 +0.8 3,031,130 - PGA1_c28890 0.21 -1.2 3,031,134 + PGA1_c28890 0.21 -1.3 3,031,167 + PGA1_c28890 0.26 -1.1 3,031,183 - PGA1_c28890 0.28 +0.2 3,031,185 + PGA1_c28890 0.28 +0.7 3,031,193 - PGA1_c28890 0.29 +0.1 3,031,295 - PGA1_c28890 0.42 +0.2 3,031,298 + PGA1_c28890 0.42 +0.2 3,031,306 - PGA1_c28890 0.43 +0.2 3,031,331 + PGA1_c28890 0.46 -0.6 3,031,331 + PGA1_c28890 0.46 -0.2 3,031,331 + PGA1_c28890 0.46 +0.0 3,031,331 + PGA1_c28890 0.46 +0.1 3,031,339 - PGA1_c28890 0.47 -0.1 3,031,339 - PGA1_c28890 0.47 -0.5 3,031,350 + PGA1_c28890 0.49 +0.2 3,031,415 + PGA1_c28890 0.57 +0.4 3,031,423 - PGA1_c28890 0.58 -0.1 3,031,428 - PGA1_c28890 0.58 -0.6 3,031,467 - PGA1_c28890 0.63 -0.1 3,031,489 - PGA1_c28890 0.66 -0.2 3,031,559 - PGA1_c28890 0.75 +1.2 3,031,703 + +0.8 3,031,739 + +1.1 3,031,774 + -2.8 3,031,786 + +0.3 3,031,793 + +0.2 3,031,807 + +1.7 3,031,815 - +0.6 3,031,815 - -0.3 3,031,815 - -1.6 3,031,828 - -2.0 3,031,856 - PGA1_c28900 0.11 +0.2 3,031,856 - PGA1_c28900 0.11 -1.0 3,031,858 - PGA1_c28900 0.11 -1.6 3,031,871 + PGA1_c28900 0.13 +0.0 3,031,871 - PGA1_c28900 0.13 +1.2 3,031,899 - PGA1_c28900 0.16 -0.6 3,031,921 + PGA1_c28900 0.18 -0.2 3,031,941 + PGA1_c28900 0.21 -1.0 3,031,949 - PGA1_c28900 0.22 -0.7 3,031,954 - PGA1_c28900 0.22 -0.6 3,031,957 + PGA1_c28900 0.22 +0.1
Or see this region's nucleotide sequence