Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20340 and PGA1_c20350 are separated by 131 nucleotides PGA1_c20350 and PGA1_c20360 are separated by 308 nucleotides
PGA1_c20340: PGA1_c20340 - Uncharacterized protein conserved in bacteria, at 2,113,778 to 2,115,001
_c20340
PGA1_c20350: PGA1_c20350 - 5-aminolevulinate synthase HemA, at 2,115,133 to 2,116,362
_c20350
PGA1_c20360: PGA1_c20360 - putative glutamate carboxypeptidase, at 2,116,671 to 2,118,062
_c20360
Position (kb)
2115
2116
2117 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2114.140 kb on - strand, within PGA1_c20340 at 2115.024 kb on + strand at 2115.106 kb on - strand at 2115.196 kb on - strand at 2115.196 kb on - strand at 2115.196 kb on - strand at 2115.196 kb on - strand at 2115.196 kb on - strand at 2116.478 kb on - strand at 2116.485 kb on - strand at 2116.497 kb on - strand at 2116.598 kb on + strand at 2116.606 kb on - strand at 2116.621 kb on + strand at 2116.623 kb on + strand at 2116.644 kb on + strand at 2116.658 kb on - strand at 2116.694 kb on - strand at 2116.736 kb on - strand at 2116.784 kb on - strand at 2116.858 kb on + strand, within PGA1_c20360 at 2116.865 kb on - strand, within PGA1_c20360 at 2116.900 kb on + strand, within PGA1_c20360 at 2116.900 kb on + strand, within PGA1_c20360 at 2116.908 kb on - strand, within PGA1_c20360 at 2116.922 kb on + strand, within PGA1_c20360 at 2116.922 kb on + strand, within PGA1_c20360 at 2116.930 kb on - strand, within PGA1_c20360 at 2116.957 kb on + strand, within PGA1_c20360 at 2117.164 kb on + strand, within PGA1_c20360 at 2117.184 kb on - strand, within PGA1_c20360 at 2117.199 kb on - strand, within PGA1_c20360 at 2117.214 kb on + strand, within PGA1_c20360 at 2117.214 kb on + strand, within PGA1_c20360 at 2117.222 kb on - strand, within PGA1_c20360 at 2117.222 kb on - strand, within PGA1_c20360 at 2117.344 kb on + strand, within PGA1_c20360 at 2117.352 kb on - strand, within PGA1_c20360
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 2,114,140 - PGA1_c20340 0.30 -0.7 2,115,024 + -2.2 2,115,106 - +0.1 2,115,196 - +0.2 2,115,196 - -0.4 2,115,196 - -0.5 2,115,196 - +2.0 2,115,196 - -1.9 2,116,478 - +0.7 2,116,485 - +0.4 2,116,497 - +0.5 2,116,598 + +0.9 2,116,606 - +0.5 2,116,621 + +0.2 2,116,623 + -0.5 2,116,644 + -0.8 2,116,658 - -1.0 2,116,694 - -1.0 2,116,736 - +0.6 2,116,784 - +0.9 2,116,858 + PGA1_c20360 0.13 -1.0 2,116,865 - PGA1_c20360 0.14 -0.8 2,116,900 + PGA1_c20360 0.16 -0.0 2,116,900 + PGA1_c20360 0.16 -1.4 2,116,908 - PGA1_c20360 0.17 -1.2 2,116,922 + PGA1_c20360 0.18 -2.3 2,116,922 + PGA1_c20360 0.18 -0.7 2,116,930 - PGA1_c20360 0.19 -0.1 2,116,957 + PGA1_c20360 0.21 -0.8 2,117,164 + PGA1_c20360 0.35 -0.4 2,117,184 - PGA1_c20360 0.37 -1.2 2,117,199 - PGA1_c20360 0.38 -0.2 2,117,214 + PGA1_c20360 0.39 -1.4 2,117,214 + PGA1_c20360 0.39 -0.8 2,117,222 - PGA1_c20360 0.40 +0.2 2,117,222 - PGA1_c20360 0.40 +1.2 2,117,344 + PGA1_c20360 0.48 -0.4 2,117,352 - PGA1_c20360 0.49 -0.1
Or see this region's nucleotide sequence