Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c17740 and PGA1_c17750 are separated by 15 nucleotides PGA1_c17750 and PGA1_c17760 overlap by 8 nucleotides PGA1_c17760 and PGA1_c17770 overlap by 4 nucleotides PGA1_c17770 and PGA1_c17780 are separated by 13 nucleotides PGA1_c17780 and PGA1_c17790 are separated by 101 nucleotides PGA1_c17790 and PGA1_c17800 overlap by 4 nucleotides
PGA1_c17740: PGA1_c17740 - putative gene transfer agent protein, at 1,858,849 to 1,859,481
_c17740
PGA1_c17750: PGA1_c17750 - putative gene transfer agent tail tape measure protein, at 1,859,497 to 1,860,153
_c17750
PGA1_c17760: PGA1_c17760 - gene transfer agent (GTA) protein, at 1,860,146 to 1,860,403
_c17760
PGA1_c17770: PGA1_c17770 - Protein of unknown function (DUF3356)., at 1,860,400 to 1,860,804
_c17770
PGA1_c17780: PGA1_c17780 - putative gene transfer agent major tail protein, at 1,860,818 to 1,861,231
_c17780
PGA1_c17790: PGA1_c17790 - putative gene transfer agent protein, at 1,861,333 to 1,861,740
_c17790
PGA1_c17800: PGA1_c17800 - putative gene transfer agent head-tail adaptor, at 1,861,737 to 1,862,108
_c17800
Position (kb)
1860
1861 Strain fitness (log2 ratio)
-2
-1
0
1 at 1859.530 kb on + strand at 1859.550 kb on + strand at 1859.550 kb on + strand at 1859.609 kb on - strand, within PGA1_c17750 at 1859.609 kb on - strand, within PGA1_c17750 at 1859.629 kb on - strand, within PGA1_c17750 at 1859.676 kb on + strand, within PGA1_c17750 at 1859.684 kb on - strand, within PGA1_c17750 at 1859.684 kb on - strand, within PGA1_c17750 at 1859.684 kb on - strand, within PGA1_c17750 at 1859.695 kb on - strand, within PGA1_c17750 at 1859.821 kb on - strand, within PGA1_c17750 at 1859.878 kb on - strand, within PGA1_c17750 at 1859.917 kb on + strand, within PGA1_c17750 at 1859.918 kb on - strand, within PGA1_c17750 at 1859.930 kb on + strand, within PGA1_c17750 at 1859.930 kb on + strand, within PGA1_c17750 at 1859.989 kb on - strand, within PGA1_c17750 at 1859.995 kb on + strand, within PGA1_c17750 at 1860.062 kb on - strand, within PGA1_c17750 at 1860.141 kb on - strand at 1860.226 kb on - strand, within PGA1_c17760 at 1860.254 kb on + strand, within PGA1_c17760 at 1860.254 kb on + strand, within PGA1_c17760 at 1860.280 kb on + strand, within PGA1_c17760 at 1860.280 kb on + strand, within PGA1_c17760 at 1860.285 kb on - strand, within PGA1_c17760 at 1860.322 kb on + strand, within PGA1_c17760 at 1860.364 kb on - strand, within PGA1_c17760 at 1860.467 kb on - strand, within PGA1_c17770 at 1860.656 kb on + strand at 1860.679 kb on + strand, within PGA1_c17770 at 1860.760 kb on - strand, within PGA1_c17770 at 1860.789 kb on + strand at 1860.797 kb on - strand at 1860.797 kb on - strand at 1860.797 kb on - strand at 1860.870 kb on - strand, within PGA1_c17780 at 1860.913 kb on + strand, within PGA1_c17780 at 1860.913 kb on + strand, within PGA1_c17780 at 1860.913 kb on + strand, within PGA1_c17780 at 1861.011 kb on - strand, within PGA1_c17780 at 1861.011 kb on - strand, within PGA1_c17780 at 1861.040 kb on + strand, within PGA1_c17780 at 1861.057 kb on + strand, within PGA1_c17780 at 1861.065 kb on - strand, within PGA1_c17780 at 1861.075 kb on + strand, within PGA1_c17780 at 1861.114 kb on - strand, within PGA1_c17780 at 1861.161 kb on - strand, within PGA1_c17780 at 1861.280 kb on + strand at 1861.280 kb on + strand at 1861.283 kb on - strand at 1861.315 kb on + strand at 1861.398 kb on - strand, within PGA1_c17790 at 1861.436 kb on - strand, within PGA1_c17790 at 1861.669 kb on + strand, within PGA1_c17790 at 1861.693 kb on - strand, within PGA1_c17790 at 1861.722 kb on + strand at 1861.728 kb on + strand at 1861.729 kb on + strand at 1861.736 kb on - strand at 1861.792 kb on + strand, within PGA1_c17800
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 1,859,530 + -0.4 1,859,550 + +0.5 1,859,550 + +0.1 1,859,609 - PGA1_c17750 0.17 +0.3 1,859,609 - PGA1_c17750 0.17 -0.3 1,859,629 - PGA1_c17750 0.20 -1.7 1,859,676 + PGA1_c17750 0.27 -0.2 1,859,684 - PGA1_c17750 0.28 +0.5 1,859,684 - PGA1_c17750 0.28 +0.6 1,859,684 - PGA1_c17750 0.28 +1.1 1,859,695 - PGA1_c17750 0.30 -1.4 1,859,821 - PGA1_c17750 0.49 -2.1 1,859,878 - PGA1_c17750 0.58 -0.4 1,859,917 + PGA1_c17750 0.64 -0.7 1,859,918 - PGA1_c17750 0.64 +0.6 1,859,930 + PGA1_c17750 0.66 -0.2 1,859,930 + PGA1_c17750 0.66 +1.1 1,859,989 - PGA1_c17750 0.75 -1.7 1,859,995 + PGA1_c17750 0.76 -1.1 1,860,062 - PGA1_c17750 0.86 -0.4 1,860,141 - -0.9 1,860,226 - PGA1_c17760 0.31 -2.6 1,860,254 + PGA1_c17760 0.42 +0.1 1,860,254 + PGA1_c17760 0.42 -1.9 1,860,280 + PGA1_c17760 0.52 -2.3 1,860,280 + PGA1_c17760 0.52 -1.4 1,860,285 - PGA1_c17760 0.54 +0.5 1,860,322 + PGA1_c17760 0.68 +0.2 1,860,364 - PGA1_c17760 0.84 -2.3 1,860,467 - PGA1_c17770 0.17 -2.1 1,860,656 + -0.4 1,860,679 + PGA1_c17770 0.69 -0.8 1,860,760 - PGA1_c17770 0.89 -1.6 1,860,789 + -0.4 1,860,797 - +0.7 1,860,797 - +0.2 1,860,797 - +0.0 1,860,870 - PGA1_c17780 0.13 +1.6 1,860,913 + PGA1_c17780 0.23 -0.9 1,860,913 + PGA1_c17780 0.23 +0.4 1,860,913 + PGA1_c17780 0.23 +0.5 1,861,011 - PGA1_c17780 0.47 -0.6 1,861,011 - PGA1_c17780 0.47 +0.7 1,861,040 + PGA1_c17780 0.54 +0.7 1,861,057 + PGA1_c17780 0.58 +0.1 1,861,065 - PGA1_c17780 0.60 +0.7 1,861,075 + PGA1_c17780 0.62 +1.1 1,861,114 - PGA1_c17780 0.71 +1.1 1,861,161 - PGA1_c17780 0.83 +1.6 1,861,280 + +1.6 1,861,280 + +1.2 1,861,283 - -0.6 1,861,315 + +0.3 1,861,398 - PGA1_c17790 0.16 +0.1 1,861,436 - PGA1_c17790 0.25 +1.7 1,861,669 + PGA1_c17790 0.82 -2.0 1,861,693 - PGA1_c17790 0.88 +0.5 1,861,722 + -0.7 1,861,728 + -0.0 1,861,729 + +1.3 1,861,736 - +0.6 1,861,792 + PGA1_c17800 0.15 -0.9
Or see this region's nucleotide sequence