Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c14460 and PGA1_c14470 are separated by 186 nucleotides PGA1_c14470 and PGA1_c14480 overlap by 4 nucleotides PGA1_c14480 and PGA1_c14490 are separated by 212 nucleotides
PGA1_c14460: PGA1_c14460 - putative phosphatidylserine decarboxylase, at 1,501,899 to 1,502,789
_c14460
PGA1_c14470: PGA1_c14470 - spermidine synthase SpeE, at 1,502,976 to 1,503,830
_c14470
PGA1_c14480: PGA1_c14480 - S-adenosylmethionine decarboxylase SpeH, at 1,503,827 to 1,504,348
_c14480
PGA1_c14490: PGA1_c14490 - Uncharacterized conserved small protein, at 1,504,561 to 1,504,794
_c14490
Position (kb) 1502
1503
1504 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1502.794 kb on - strand at 1502.870 kb on + strand at 1502.878 kb on - strand at 1502.906 kb on - strand at 1503.009 kb on + strand at 1503.037 kb on - strand at 1503.100 kb on - strand, within PGA1_c14470 at 1503.247 kb on - strand, within PGA1_c14470 at 1503.281 kb on - strand, within PGA1_c14470 at 1503.313 kb on + strand, within PGA1_c14470 at 1503.448 kb on - strand, within PGA1_c14470 at 1503.557 kb on - strand, within PGA1_c14470 at 1503.674 kb on - strand, within PGA1_c14470 at 1503.674 kb on - strand, within PGA1_c14470 at 1503.674 kb on - strand, within PGA1_c14470 at 1503.679 kb on - strand, within PGA1_c14470 at 1503.712 kb on + strand, within PGA1_c14470 at 1503.742 kb on + strand, within PGA1_c14470 at 1503.849 kb on - strand at 1503.849 kb on - strand at 1503.849 kb on - strand at 1503.895 kb on + strand, within PGA1_c14480 at 1503.895 kb on + strand, within PGA1_c14480 at 1503.903 kb on - strand, within PGA1_c14480 at 1503.903 kb on - strand, within PGA1_c14480 at 1503.903 kb on - strand, within PGA1_c14480 at 1503.903 kb on - strand, within PGA1_c14480 at 1503.949 kb on + strand, within PGA1_c14480 at 1503.965 kb on + strand, within PGA1_c14480 at 1503.981 kb on - strand, within PGA1_c14480 at 1504.098 kb on + strand, within PGA1_c14480 at 1504.156 kb on - strand, within PGA1_c14480 at 1504.183 kb on + strand, within PGA1_c14480 at 1504.193 kb on + strand, within PGA1_c14480 at 1504.215 kb on - strand, within PGA1_c14480 at 1504.266 kb on + strand, within PGA1_c14480 at 1504.294 kb on - strand, within PGA1_c14480 at 1504.307 kb on + strand at 1504.332 kb on + strand at 1504.412 kb on - strand at 1504.498 kb on + strand at 1504.506 kb on - strand at 1504.506 kb on - strand at 1504.506 kb on - strand at 1504.533 kb on + strand at 1504.552 kb on + strand at 1504.632 kb on + strand, within PGA1_c14490 at 1504.677 kb on - strand, within PGA1_c14490 at 1504.698 kb on + strand, within PGA1_c14490 at 1504.717 kb on - strand, within PGA1_c14490
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 1,502,794 - -1.4 1,502,870 + +1.2 1,502,878 - -0.0 1,502,906 - +0.6 1,503,009 + -1.8 1,503,037 - -2.4 1,503,100 - PGA1_c14470 0.15 -0.1 1,503,247 - PGA1_c14470 0.32 -0.3 1,503,281 - PGA1_c14470 0.36 -0.2 1,503,313 + PGA1_c14470 0.39 -2.1 1,503,448 - PGA1_c14470 0.55 -1.4 1,503,557 - PGA1_c14470 0.68 -1.4 1,503,674 - PGA1_c14470 0.82 -0.8 1,503,674 - PGA1_c14470 0.82 -0.3 1,503,674 - PGA1_c14470 0.82 -2.0 1,503,679 - PGA1_c14470 0.82 -0.8 1,503,712 + PGA1_c14470 0.86 -3.3 1,503,742 + PGA1_c14470 0.90 +0.0 1,503,849 - -0.5 1,503,849 - +1.0 1,503,849 - +1.2 1,503,895 + PGA1_c14480 0.13 -0.3 1,503,895 + PGA1_c14480 0.13 -0.6 1,503,903 - PGA1_c14480 0.15 +0.2 1,503,903 - PGA1_c14480 0.15 -0.4 1,503,903 - PGA1_c14480 0.15 -1.6 1,503,903 - PGA1_c14480 0.15 +0.9 1,503,949 + PGA1_c14480 0.23 +0.4 1,503,965 + PGA1_c14480 0.26 -1.7 1,503,981 - PGA1_c14480 0.30 -0.4 1,504,098 + PGA1_c14480 0.52 -0.0 1,504,156 - PGA1_c14480 0.63 +0.8 1,504,183 + PGA1_c14480 0.68 -0.2 1,504,193 + PGA1_c14480 0.70 -1.0 1,504,215 - PGA1_c14480 0.74 -0.4 1,504,266 + PGA1_c14480 0.84 -1.2 1,504,294 - PGA1_c14480 0.89 -2.4 1,504,307 + -0.1 1,504,332 + +0.2 1,504,412 - -1.7 1,504,498 + -1.0 1,504,506 - +0.1 1,504,506 - +0.2 1,504,506 - +0.9 1,504,533 + -0.2 1,504,552 + +0.4 1,504,632 + PGA1_c14490 0.30 +0.8 1,504,677 - PGA1_c14490 0.50 +1.4 1,504,698 + PGA1_c14490 0.59 -0.2 1,504,717 - PGA1_c14490 0.67 +0.6
Or see this region's nucleotide sequence