Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c13190 and PGA1_c13200 are separated by 9 nucleotides PGA1_c13200 and PGA1_c13210 are separated by 116 nucleotides PGA1_c13210 and PGA1_c13220 are separated by 114 nucleotides
PGA1_c13190: PGA1_c13190 - ABC transporter for D-Sorbitol, permease component 1 (from data) , at 1,364,851 to 1,365,681
_c13190
PGA1_c13200: PGA1_c13200 - ABC transporter for D-Sorbitol, permease component 2 (from data) , at 1,365,691 to 1,366,557
_c13200
PGA1_c13210: PGA1_c13210 - ABC transporter for D-Sorbitol, periplasmic substrate-binding component (from data) , at 1,366,674 to 1,367,975
_c13210
PGA1_c13220: PGA1_c13220 - putative transcriptional regulator, lacI family, at 1,368,090 to 1,369,121
_c13220
Position (kb)
1366
1367
1368 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1365.690 kb on + strand at 1365.826 kb on + strand, within PGA1_c13200 at 1365.849 kb on + strand, within PGA1_c13200 at 1365.995 kb on + strand, within PGA1_c13200 at 1365.995 kb on + strand, within PGA1_c13200 at 1366.003 kb on - strand, within PGA1_c13200 at 1366.003 kb on - strand, within PGA1_c13200 at 1366.003 kb on - strand, within PGA1_c13200 at 1366.039 kb on + strand, within PGA1_c13200 at 1366.047 kb on - strand, within PGA1_c13200 at 1366.100 kb on + strand, within PGA1_c13200 at 1366.100 kb on + strand, within PGA1_c13200 at 1366.103 kb on + strand, within PGA1_c13200 at 1366.117 kb on + strand, within PGA1_c13200 at 1366.197 kb on + strand, within PGA1_c13200 at 1366.311 kb on - strand, within PGA1_c13200 at 1366.344 kb on - strand, within PGA1_c13200 at 1366.347 kb on + strand, within PGA1_c13200 at 1366.355 kb on - strand, within PGA1_c13200 at 1366.401 kb on + strand, within PGA1_c13200 at 1366.411 kb on + strand, within PGA1_c13200 at 1366.411 kb on + strand, within PGA1_c13200 at 1366.424 kb on - strand, within PGA1_c13200 at 1366.427 kb on + strand, within PGA1_c13200 at 1366.480 kb on - strand at 1366.629 kb on + strand at 1366.633 kb on - strand at 1366.653 kb on + strand at 1366.661 kb on - strand at 1366.688 kb on + strand at 1366.705 kb on - strand at 1366.717 kb on + strand at 1366.778 kb on - strand at 1366.778 kb on - strand at 1366.778 kb on - strand at 1366.784 kb on + strand at 1366.821 kb on - strand, within PGA1_c13210 at 1366.868 kb on - strand, within PGA1_c13210 at 1366.889 kb on - strand, within PGA1_c13210 at 1366.913 kb on - strand, within PGA1_c13210 at 1366.920 kb on - strand, within PGA1_c13210 at 1366.928 kb on - strand, within PGA1_c13210 at 1366.991 kb on + strand, within PGA1_c13210 at 1366.991 kb on + strand, within PGA1_c13210 at 1366.999 kb on - strand, within PGA1_c13210 at 1366.999 kb on - strand, within PGA1_c13210 at 1367.021 kb on + strand, within PGA1_c13210 at 1367.111 kb on - strand, within PGA1_c13210 at 1367.212 kb on + strand, within PGA1_c13210 at 1367.212 kb on + strand, within PGA1_c13210 at 1367.322 kb on + strand, within PGA1_c13210 at 1367.330 kb on - strand, within PGA1_c13210 at 1367.402 kb on - strand, within PGA1_c13210 at 1367.510 kb on - strand, within PGA1_c13210 at 1367.678 kb on + strand, within PGA1_c13210 at 1367.686 kb on - strand, within PGA1_c13210 at 1367.686 kb on - strand, within PGA1_c13210 at 1367.686 kb on - strand, within PGA1_c13210 at 1367.752 kb on - strand, within PGA1_c13210 at 1367.752 kb on - strand, within PGA1_c13210 at 1367.808 kb on - strand, within PGA1_c13210 at 1367.846 kb on + strand at 1367.950 kb on - strand at 1367.969 kb on + strand at 1367.970 kb on - strand at 1367.977 kb on - strand at 1368.095 kb on - strand at 1368.181 kb on - strand at 1368.185 kb on + strand at 1368.208 kb on + strand, within PGA1_c13220 at 1368.216 kb on - strand, within PGA1_c13220 at 1368.233 kb on + strand, within PGA1_c13220 at 1368.241 kb on - strand, within PGA1_c13220 at 1368.299 kb on + strand, within PGA1_c13220 at 1368.325 kb on - strand, within PGA1_c13220 at 1368.331 kb on + strand, within PGA1_c13220 at 1368.334 kb on + strand, within PGA1_c13220 at 1368.364 kb on + strand, within PGA1_c13220 at 1368.463 kb on + strand, within PGA1_c13220 at 1368.471 kb on - strand, within PGA1_c13220 at 1368.507 kb on + strand, within PGA1_c13220 at 1368.532 kb on + strand, within PGA1_c13220 at 1368.532 kb on + strand, within PGA1_c13220 at 1368.532 kb on + strand, within PGA1_c13220 at 1368.532 kb on + strand, within PGA1_c13220 at 1368.636 kb on + strand, within PGA1_c13220 at 1368.644 kb on - strand, within PGA1_c13220 at 1368.644 kb on - strand, within PGA1_c13220 at 1368.685 kb on - strand, within PGA1_c13220 at 1368.690 kb on - strand, within PGA1_c13220 at 1368.708 kb on + strand, within PGA1_c13220 at 1368.768 kb on - strand, within PGA1_c13220 at 1368.770 kb on + strand, within PGA1_c13220 at 1368.822 kb on + strand, within PGA1_c13220 at 1368.830 kb on - strand, within PGA1_c13220 at 1368.925 kb on + strand, within PGA1_c13220 at 1368.925 kb on + strand, within PGA1_c13220
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 1,365,690 + +1.3 1,365,826 + PGA1_c13200 0.16 -1.0 1,365,849 + PGA1_c13200 0.18 +0.6 1,365,995 + PGA1_c13200 0.35 -0.6 1,365,995 + PGA1_c13200 0.35 -0.5 1,366,003 - PGA1_c13200 0.36 +1.2 1,366,003 - PGA1_c13200 0.36 +0.5 1,366,003 - PGA1_c13200 0.36 +0.5 1,366,039 + PGA1_c13200 0.40 -0.8 1,366,047 - PGA1_c13200 0.41 -1.0 1,366,100 + PGA1_c13200 0.47 +0.2 1,366,100 + PGA1_c13200 0.47 +0.5 1,366,103 + PGA1_c13200 0.48 +0.5 1,366,117 + PGA1_c13200 0.49 +1.8 1,366,197 + PGA1_c13200 0.58 +0.8 1,366,311 - PGA1_c13200 0.72 -0.4 1,366,344 - PGA1_c13200 0.75 +1.2 1,366,347 + PGA1_c13200 0.76 +1.5 1,366,355 - PGA1_c13200 0.77 -0.4 1,366,401 + PGA1_c13200 0.82 +0.8 1,366,411 + PGA1_c13200 0.83 -0.6 1,366,411 + PGA1_c13200 0.83 -1.2 1,366,424 - PGA1_c13200 0.85 +1.0 1,366,427 + PGA1_c13200 0.85 -0.2 1,366,480 - +0.4 1,366,629 + +0.5 1,366,633 - +0.1 1,366,653 + -1.1 1,366,661 - +1.0 1,366,688 + +0.2 1,366,705 - +0.2 1,366,717 + -0.3 1,366,778 - +0.1 1,366,778 - +1.4 1,366,778 - +0.6 1,366,784 + +1.3 1,366,821 - PGA1_c13210 0.11 -0.3 1,366,868 - PGA1_c13210 0.15 -0.1 1,366,889 - PGA1_c13210 0.17 -0.1 1,366,913 - PGA1_c13210 0.18 -0.7 1,366,920 - PGA1_c13210 0.19 -0.9 1,366,928 - PGA1_c13210 0.20 -1.2 1,366,991 + PGA1_c13210 0.24 -0.8 1,366,991 + PGA1_c13210 0.24 -1.0 1,366,999 - PGA1_c13210 0.25 -0.1 1,366,999 - PGA1_c13210 0.25 -1.0 1,367,021 + PGA1_c13210 0.27 +1.2 1,367,111 - PGA1_c13210 0.34 -2.5 1,367,212 + PGA1_c13210 0.41 +1.2 1,367,212 + PGA1_c13210 0.41 +1.6 1,367,322 + PGA1_c13210 0.50 -0.4 1,367,330 - PGA1_c13210 0.50 +0.7 1,367,402 - PGA1_c13210 0.56 -0.8 1,367,510 - PGA1_c13210 0.64 -1.2 1,367,678 + PGA1_c13210 0.77 -1.1 1,367,686 - PGA1_c13210 0.78 +0.5 1,367,686 - PGA1_c13210 0.78 +1.4 1,367,686 - PGA1_c13210 0.78 -0.6 1,367,752 - PGA1_c13210 0.83 -0.1 1,367,752 - PGA1_c13210 0.83 -0.4 1,367,808 - PGA1_c13210 0.87 +0.6 1,367,846 + +0.3 1,367,950 - +0.2 1,367,969 + +0.9 1,367,970 - -0.2 1,367,977 - -1.4 1,368,095 - -0.5 1,368,181 - +0.3 1,368,185 + +0.7 1,368,208 + PGA1_c13220 0.11 +0.0 1,368,216 - PGA1_c13220 0.12 -0.7 1,368,233 + PGA1_c13220 0.14 -0.7 1,368,241 - PGA1_c13220 0.15 +1.3 1,368,299 + PGA1_c13220 0.20 -0.1 1,368,325 - PGA1_c13220 0.23 -0.7 1,368,331 + PGA1_c13220 0.23 +0.7 1,368,334 + PGA1_c13220 0.24 +0.5 1,368,364 + PGA1_c13220 0.27 +1.2 1,368,463 + PGA1_c13220 0.36 -0.3 1,368,471 - PGA1_c13220 0.37 -1.4 1,368,507 + PGA1_c13220 0.40 -0.6 1,368,532 + PGA1_c13220 0.43 +0.8 1,368,532 + PGA1_c13220 0.43 +1.5 1,368,532 + PGA1_c13220 0.43 +2.0 1,368,532 + PGA1_c13220 0.43 +3.1 1,368,636 + PGA1_c13220 0.53 +0.4 1,368,644 - PGA1_c13220 0.54 -1.1 1,368,644 - PGA1_c13220 0.54 +0.6 1,368,685 - PGA1_c13220 0.58 +1.0 1,368,690 - PGA1_c13220 0.58 +0.4 1,368,708 + PGA1_c13220 0.60 +0.3 1,368,768 - PGA1_c13220 0.66 +1.9 1,368,770 + PGA1_c13220 0.66 -0.4 1,368,822 + PGA1_c13220 0.71 +0.6 1,368,830 - PGA1_c13220 0.72 +1.8 1,368,925 + PGA1_c13220 0.81 +1.2 1,368,925 + PGA1_c13220 0.81 -0.5
Or see this region's nucleotide sequence