Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11030 and PGA1_c11040 are separated by 169 nucleotides PGA1_c11040 and PGA1_c11050 are separated by 119 nucleotides PGA1_c11050 and PGA1_c11060 are separated by 215 nucleotides PGA1_c11060 and PGA1_c11070 are separated by 206 nucleotides
PGA1_c11030: PGA1_c11030 - MoxR-like ATPases, at 1,141,883 to 1,142,722
_c11030
PGA1_c11040: PGA1_c11040 - dnaK suppressor protein DksA, at 1,142,892 to 1,143,338
_c11040
PGA1_c11050: PGA1_c11050 - FAD dependent oxidoreductase, at 1,143,458 to 1,144,684
_c11050
PGA1_c11060: PGA1_c11060 - cold shock-like protein CspE, at 1,144,900 to 1,145,106
_c11060
PGA1_c11070: PGA1_c11070 - exodeoxyribonuclease III, at 1,145,313 to 1,146,119
_c11070
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1142.577 kb on - strand, within PGA1_c11030 at 1142.618 kb on - strand, within PGA1_c11030 at 1142.625 kb on + strand, within PGA1_c11030 at 1142.648 kb on - strand at 1142.702 kb on - strand at 1142.702 kb on - strand at 1142.741 kb on + strand at 1142.751 kb on + strand at 1142.782 kb on - strand at 1142.797 kb on + strand at 1142.800 kb on - strand at 1142.805 kb on - strand at 1142.848 kb on - strand at 1142.876 kb on + strand at 1142.890 kb on + strand at 1142.890 kb on + strand at 1142.890 kb on + strand at 1142.890 kb on + strand at 1142.890 kb on + strand at 1142.898 kb on - strand at 1142.898 kb on - strand at 1142.898 kb on - strand at 1142.900 kb on + strand at 1142.900 kb on + strand at 1142.903 kb on - strand at 1142.908 kb on - strand at 1142.908 kb on - strand at 1142.955 kb on + strand, within PGA1_c11040 at 1142.963 kb on - strand, within PGA1_c11040 at 1142.975 kb on - strand, within PGA1_c11040 at 1142.977 kb on - strand, within PGA1_c11040 at 1143.011 kb on + strand, within PGA1_c11040 at 1143.019 kb on - strand, within PGA1_c11040 at 1143.076 kb on - strand, within PGA1_c11040 at 1143.127 kb on + strand, within PGA1_c11040 at 1143.127 kb on + strand, within PGA1_c11040 at 1143.135 kb on - strand, within PGA1_c11040 at 1143.204 kb on + strand, within PGA1_c11040 at 1143.217 kb on + strand, within PGA1_c11040 at 1143.225 kb on - strand, within PGA1_c11040 at 1143.234 kb on + strand, within PGA1_c11040 at 1143.237 kb on - strand, within PGA1_c11040 at 1143.283 kb on + strand, within PGA1_c11040 at 1143.291 kb on - strand, within PGA1_c11040 at 1143.291 kb on - strand, within PGA1_c11040 at 1143.377 kb on - strand at 1143.385 kb on - strand at 1143.419 kb on + strand at 1143.433 kb on + strand at 1144.634 kb on - strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.678 kb on + strand at 1144.686 kb on - strand at 1144.686 kb on - strand at 1144.765 kb on + strand at 1144.765 kb on + strand at 1144.819 kb on + strand at 1144.827 kb on - strand at 1144.839 kb on + strand at 1144.892 kb on + strand at 1144.963 kb on + strand, within PGA1_c11060 at 1144.970 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.011 kb on + strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.019 kb on - strand, within PGA1_c11060 at 1145.136 kb on + strand at 1145.203 kb on - strand at 1145.219 kb on + strand at 1145.219 kb on + strand at 1145.227 kb on - strand at 1145.227 kb on - strand at 1145.227 kb on - strand at 1145.332 kb on - strand at 1145.503 kb on - strand, within PGA1_c11070 at 1145.620 kb on + strand, within PGA1_c11070
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 1,142,577 - PGA1_c11030 0.83 -0.6 1,142,618 - PGA1_c11030 0.88 -0.8 1,142,625 + PGA1_c11030 0.88 -0.0 1,142,648 - -0.0 1,142,702 - -1.6 1,142,702 - -2.3 1,142,741 + +0.6 1,142,751 + -0.4 1,142,782 - +2.2 1,142,797 + +0.2 1,142,800 - -0.3 1,142,805 - +1.2 1,142,848 - -1.4 1,142,876 + -1.0 1,142,890 + -2.9 1,142,890 + -2.1 1,142,890 + -3.5 1,142,890 + -1.4 1,142,890 + -0.4 1,142,898 - -3.8 1,142,898 - -1.8 1,142,898 - -2.3 1,142,900 + -1.1 1,142,900 + -2.1 1,142,903 - -3.2 1,142,908 - -1.6 1,142,908 - -2.6 1,142,955 + PGA1_c11040 0.14 -1.1 1,142,963 - PGA1_c11040 0.16 -1.8 1,142,975 - PGA1_c11040 0.19 -2.5 1,142,977 - PGA1_c11040 0.19 -2.5 1,143,011 + PGA1_c11040 0.27 -2.0 1,143,019 - PGA1_c11040 0.28 -1.4 1,143,076 - PGA1_c11040 0.41 -2.4 1,143,127 + PGA1_c11040 0.53 -2.8 1,143,127 + PGA1_c11040 0.53 -1.6 1,143,135 - PGA1_c11040 0.54 -2.1 1,143,204 + PGA1_c11040 0.70 -3.3 1,143,217 + PGA1_c11040 0.73 -1.1 1,143,225 - PGA1_c11040 0.74 -1.8 1,143,234 + PGA1_c11040 0.77 -1.1 1,143,237 - PGA1_c11040 0.77 -1.4 1,143,283 + PGA1_c11040 0.87 -1.6 1,143,291 - PGA1_c11040 0.89 -1.1 1,143,291 - PGA1_c11040 0.89 -0.4 1,143,377 - -0.1 1,143,385 - +0.8 1,143,419 + +0.2 1,143,433 + -0.6 1,144,634 - -1.6 1,144,678 + +2.8 1,144,678 + +0.9 1,144,678 + +0.4 1,144,678 + -0.7 1,144,678 + +0.5 1,144,678 + +2.2 1,144,678 + +0.9 1,144,678 + +0.8 1,144,678 + -1.1 1,144,686 - -0.6 1,144,686 - -0.1 1,144,765 + +0.3 1,144,765 + +0.4 1,144,819 + +1.2 1,144,827 - +0.0 1,144,839 + +0.5 1,144,892 + +2.2 1,144,963 + PGA1_c11060 0.30 -0.7 1,144,970 + PGA1_c11060 0.34 -0.4 1,145,011 + PGA1_c11060 0.54 +1.8 1,145,011 + PGA1_c11060 0.54 +0.9 1,145,011 + PGA1_c11060 0.54 -1.6 1,145,011 + PGA1_c11060 0.54 -0.0 1,145,011 + PGA1_c11060 0.54 +0.2 1,145,011 + PGA1_c11060 0.54 -0.6 1,145,011 + PGA1_c11060 0.54 -1.4 1,145,011 + PGA1_c11060 0.54 +1.4 1,145,011 + PGA1_c11060 0.54 -1.3 1,145,011 + PGA1_c11060 0.54 -0.8 1,145,011 + PGA1_c11060 0.54 -0.0 1,145,011 + PGA1_c11060 0.54 +1.1 1,145,011 + PGA1_c11060 0.54 -0.1 1,145,019 - PGA1_c11060 0.57 -0.7 1,145,019 - PGA1_c11060 0.57 +0.6 1,145,019 - PGA1_c11060 0.57 +0.3 1,145,019 - PGA1_c11060 0.57 +0.2 1,145,019 - PGA1_c11060 0.57 +0.2 1,145,019 - PGA1_c11060 0.57 +0.6 1,145,019 - PGA1_c11060 0.57 +0.3 1,145,136 + +1.0 1,145,203 - +0.7 1,145,219 + +0.5 1,145,219 + +0.8 1,145,227 - -0.5 1,145,227 - -0.2 1,145,227 - -0.1 1,145,332 - -1.6 1,145,503 - PGA1_c11070 0.24 -1.1 1,145,620 + PGA1_c11070 0.38 -0.6
Or see this region's nucleotide sequence