Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11220 and BWI76_RS11225 are separated by 48 nucleotides BWI76_RS11225 and BWI76_RS11230 are separated by 14 nucleotides BWI76_RS11230 and BWI76_RS11235 are separated by 28 nucleotides BWI76_RS11235 and BWI76_RS11240 are separated by 346 nucleotides
BWI76_RS11220: BWI76_RS11220 - lipoprotein transporter subunit LolE, at 2,367,462 to 2,368,706
_RS11220
BWI76_RS11225: BWI76_RS11225 - N-acetylglucosamine kinase, at 2,368,755 to 2,369,666
_RS11225
BWI76_RS11230: BWI76_RS11230 - NAD-dependent deacylase, at 2,369,681 to 2,370,526
_RS11230
BWI76_RS11235: BWI76_RS11235 - hypothetical protein, at 2,370,555 to 2,370,860
_RS11235
BWI76_RS11240: BWI76_RS11240 - spermidine/putrescine ABC transporter substrate-binding protein PotD, at 2,371,207 to 2,372,253
_RS11240
Position (kb)
2369
2370
2371 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2368.804 kb on - strand at 2369.610 kb on + strand at 2369.628 kb on + strand at 2369.636 kb on - strand at 2369.735 kb on + strand at 2369.988 kb on - strand, within BWI76_RS11230 at 2370.211 kb on - strand, within BWI76_RS11230 at 2370.335 kb on - strand, within BWI76_RS11230 at 2370.442 kb on + strand at 2370.552 kb on + strand at 2370.560 kb on - strand at 2370.623 kb on + strand, within BWI76_RS11235 at 2370.904 kb on + strand at 2371.030 kb on + strand at 2371.166 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 2,368,804 - -1.3 2,369,610 + +2.5 2,369,628 + -0.5 2,369,636 - +0.2 2,369,735 + -2.0 2,369,988 - BWI76_RS11230 0.36 +1.0 2,370,211 - BWI76_RS11230 0.63 +1.2 2,370,335 - BWI76_RS11230 0.77 +0.6 2,370,442 + +0.8 2,370,552 + +1.4 2,370,560 - +0.2 2,370,623 + BWI76_RS11235 0.22 -2.9 2,370,904 + +0.1 2,371,030 + +0.1 2,371,166 - +5.7
Or see this region's nucleotide sequence