Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03020 and BWI76_RS03025 are separated by 99 nucleotides BWI76_RS03025 and BWI76_RS03030 are separated by 123 nucleotides BWI76_RS03030 and BWI76_RS03035 overlap by 1 nucleotides BWI76_RS03035 and BWI76_RS03040 are separated by 98 nucleotides
BWI76_RS03020: BWI76_RS03020 - tRNA hydroxylase, at 617,545 to 618,303
_RS03020
BWI76_RS03025: BWI76_RS03025 - N-acetyltransferase, at 618,403 to 618,906
_RS03025
BWI76_RS03030: BWI76_RS03030 - valine--tRNA ligase, at 619,030 to 621,885
_RS03030
BWI76_RS03035: BWI76_RS03035 - DNA polymerase III subunit chi, at 621,885 to 622,328
_RS03035
BWI76_RS03040: BWI76_RS03040 - leucyl aminopeptidase, at 622,427 to 623,938
_RS03040
Position (kb) 619
620
621
622 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 618.331 kb on - strand at 618.410 kb on + strand at 618.565 kb on + strand, within BWI76_RS03025 at 618.573 kb on - strand, within BWI76_RS03025 at 618.605 kb on - strand, within BWI76_RS03025 at 618.884 kb on - strand at 618.885 kb on - strand at 618.966 kb on + strand at 622.466 kb on - strand at 622.850 kb on - strand, within BWI76_RS03040
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 618,331 - +0.9 618,410 + +0.2 618,565 + BWI76_RS03025 0.32 -0.9 618,573 - BWI76_RS03025 0.34 -0.4 618,605 - BWI76_RS03025 0.40 +5.0 618,884 - -2.1 618,885 - -0.4 618,966 + -0.8 622,466 - +1.5 622,850 - BWI76_RS03040 0.28 +1.1
Or see this region's nucleotide sequence