Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02195 and BWI76_RS02200 overlap by 4 nucleotides BWI76_RS02200 and BWI76_RS02205 are separated by 196 nucleotides BWI76_RS02205 and BWI76_RS02210 overlap by 4 nucleotides BWI76_RS02210 and BWI76_RS02215 overlap by 8 nucleotides
BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881
_RS02195
BWI76_RS02200: BWI76_RS02200 - phosphonate C-P lyase system protein PhnL, at 455,878 to 456,564
_RS02200
BWI76_RS02205: BWI76_RS02205 - phosphonate C-P lyase system protein PhnK, at 456,761 to 457,519
_RS02205
BWI76_RS02210: BWI76_RS02210 - carbon-phosphorus lyase complex subunit PhnJ, at 457,516 to 458,364
_RS02210
BWI76_RS02215: BWI76_RS02215 - carbon-phosphorus lyase complex subunit PhnI, at 458,357 to 459,421
_RS02215
Position (kb) 456
457
458 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 455.860 kb on + strand at 455.860 kb on + strand at 455.868 kb on - strand at 455.904 kb on + strand at 456.015 kb on - strand, within BWI76_RS02200 at 456.016 kb on + strand, within BWI76_RS02200 at 456.268 kb on - strand, within BWI76_RS02200 at 456.309 kb on - strand, within BWI76_RS02200 at 456.316 kb on - strand, within BWI76_RS02200 at 456.316 kb on - strand, within BWI76_RS02200 at 456.326 kb on - strand, within BWI76_RS02200 at 456.439 kb on + strand, within BWI76_RS02200 at 456.648 kb on - strand at 456.716 kb on + strand at 456.724 kb on - strand at 456.747 kb on + strand at 456.795 kb on + strand at 456.795 kb on + strand at 456.803 kb on - strand at 456.931 kb on + strand, within BWI76_RS02205 at 457.034 kb on + strand, within BWI76_RS02205 at 457.064 kb on - strand, within BWI76_RS02205 at 457.074 kb on - strand, within BWI76_RS02205 at 457.164 kb on + strand, within BWI76_RS02205 at 457.164 kb on + strand, within BWI76_RS02205 at 457.194 kb on - strand, within BWI76_RS02205 at 457.436 kb on + strand, within BWI76_RS02205 at 457.473 kb on + strand at 457.637 kb on + strand, within BWI76_RS02210 at 457.654 kb on + strand, within BWI76_RS02210 at 457.701 kb on + strand at 457.718 kb on + strand, within BWI76_RS02210 at 457.752 kb on + strand, within BWI76_RS02210 at 457.760 kb on - strand, within BWI76_RS02210 at 457.826 kb on + strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.854 kb on - strand, within BWI76_RS02210 at 457.925 kb on - strand, within BWI76_RS02210 at 457.974 kb on + strand, within BWI76_RS02210 at 458.223 kb on - strand, within BWI76_RS02210 at 458.385 kb on + strand at 458.385 kb on + strand at 458.393 kb on - strand at 458.393 kb on - strand at 458.393 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 455,860 + -0.7 455,860 + -0.1 455,868 - +0.7 455,904 + -1.6 456,015 - BWI76_RS02200 0.20 -1.3 456,016 + BWI76_RS02200 0.20 +2.2 456,268 - BWI76_RS02200 0.57 -0.7 456,309 - BWI76_RS02200 0.63 -0.2 456,316 - BWI76_RS02200 0.64 -0.4 456,316 - BWI76_RS02200 0.64 -0.3 456,326 - BWI76_RS02200 0.65 -0.4 456,439 + BWI76_RS02200 0.82 +1.3 456,648 - -0.2 456,716 + -0.4 456,724 - +0.0 456,747 + -0.3 456,795 + +0.7 456,795 + -0.2 456,803 - -0.2 456,931 + BWI76_RS02205 0.22 +0.0 457,034 + BWI76_RS02205 0.36 +0.9 457,064 - BWI76_RS02205 0.40 +0.5 457,074 - BWI76_RS02205 0.41 +0.3 457,164 + BWI76_RS02205 0.53 -0.2 457,164 + BWI76_RS02205 0.53 +3.2 457,194 - BWI76_RS02205 0.57 +1.5 457,436 + BWI76_RS02205 0.89 +1.2 457,473 + +0.4 457,637 + BWI76_RS02210 0.14 +0.7 457,654 + BWI76_RS02210 0.16 -0.1 457,701 + +3.5 457,718 + BWI76_RS02210 0.24 -0.4 457,752 + BWI76_RS02210 0.28 +0.9 457,760 - BWI76_RS02210 0.29 +0.1 457,826 + BWI76_RS02210 0.37 +0.9 457,854 - BWI76_RS02210 0.40 -1.4 457,854 - BWI76_RS02210 0.40 +0.5 457,854 - BWI76_RS02210 0.40 +1.5 457,925 - BWI76_RS02210 0.48 -2.1 457,974 + BWI76_RS02210 0.54 +0.6 458,223 - BWI76_RS02210 0.83 -0.2 458,385 + +1.4 458,385 + +1.1 458,393 - -0.4 458,393 - -0.7 458,393 - +0.6
Or see this region's nucleotide sequence