Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c10100 and PGA1_c10110 overlap by 8 nucleotides PGA1_c10110 and PGA1_c10120 are separated by 149 nucleotides
PGA1_c10100: PGA1_c10100 - putative glutathione transport system permease protein GsiD, at 1,042,875 to 1,043,846
_c10100
PGA1_c10110: PGA1_c10110 - glutathione transport system permease protein GsiC, at 1,043,839 to 1,044,846
_c10110
PGA1_c10120: PGA1_c10120 - putative glutathione-binding protein GsiB, at 1,044,996 to 1,046,696
_c10120
Position (kb)
1043
1044
1045 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1042.840 kb on - strand at 1043.283 kb on + strand, within PGA1_c10100 at 1043.309 kb on + strand, within PGA1_c10100 at 1043.427 kb on - strand, within PGA1_c10100 at 1043.483 kb on + strand, within PGA1_c10100 at 1043.581 kb on - strand, within PGA1_c10100 at 1043.737 kb on - strand, within PGA1_c10100 at 1043.779 kb on + strand at 1043.797 kb on - strand at 1043.832 kb on + strand at 1043.971 kb on + strand, within PGA1_c10110 at 1044.009 kb on + strand, within PGA1_c10110 at 1044.046 kb on + strand, within PGA1_c10110 at 1044.171 kb on + strand, within PGA1_c10110 at 1044.199 kb on - strand, within PGA1_c10110 at 1044.201 kb on - strand, within PGA1_c10110 at 1044.267 kb on + strand, within PGA1_c10110 at 1044.275 kb on - strand, within PGA1_c10110 at 1044.314 kb on + strand, within PGA1_c10110 at 1044.353 kb on - strand, within PGA1_c10110 at 1044.390 kb on + strand, within PGA1_c10110 at 1044.406 kb on - strand, within PGA1_c10110 at 1044.416 kb on - strand, within PGA1_c10110 at 1044.485 kb on - strand, within PGA1_c10110 at 1044.733 kb on + strand, within PGA1_c10110 at 1044.741 kb on - strand, within PGA1_c10110 at 1044.802 kb on + strand at 1044.811 kb on + strand at 1044.816 kb on + strand at 1044.824 kb on - strand at 1044.846 kb on - strand at 1044.850 kb on + strand at 1044.877 kb on + strand at 1044.877 kb on + strand at 1044.895 kb on - strand at 1044.977 kb on - strand at 1045.016 kb on - strand at 1045.055 kb on - strand at 1045.077 kb on - strand at 1045.096 kb on + strand at 1045.140 kb on + strand at 1045.198 kb on + strand, within PGA1_c10120 at 1045.393 kb on - strand, within PGA1_c10120 at 1045.408 kb on + strand, within PGA1_c10120 at 1045.447 kb on + strand, within PGA1_c10120 at 1045.523 kb on - strand, within PGA1_c10120 at 1045.535 kb on - strand, within PGA1_c10120 at 1045.535 kb on - strand, within PGA1_c10120 at 1045.537 kb on - strand, within PGA1_c10120 at 1045.549 kb on + strand, within PGA1_c10120 at 1045.564 kb on - strand, within PGA1_c10120 at 1045.726 kb on + strand, within PGA1_c10120 at 1045.796 kb on - strand, within PGA1_c10120 at 1045.801 kb on - strand, within PGA1_c10120 at 1045.816 kb on + strand, within PGA1_c10120 at 1045.816 kb on + strand, within PGA1_c10120 at 1045.824 kb on - strand, within PGA1_c10120
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 1,042,840 - -1.7 1,043,283 + PGA1_c10100 0.42 +0.6 1,043,309 + PGA1_c10100 0.45 -0.8 1,043,427 - PGA1_c10100 0.57 -1.2 1,043,483 + PGA1_c10100 0.63 -2.1 1,043,581 - PGA1_c10100 0.73 -2.6 1,043,737 - PGA1_c10100 0.89 -1.0 1,043,779 + -1.4 1,043,797 - -0.2 1,043,832 + -1.0 1,043,971 + PGA1_c10110 0.13 -1.4 1,044,009 + PGA1_c10110 0.17 -0.4 1,044,046 + PGA1_c10110 0.21 -1.7 1,044,171 + PGA1_c10110 0.33 -1.8 1,044,199 - PGA1_c10110 0.36 -1.6 1,044,201 - PGA1_c10110 0.36 -0.8 1,044,267 + PGA1_c10110 0.42 -0.4 1,044,275 - PGA1_c10110 0.43 -0.6 1,044,314 + PGA1_c10110 0.47 -0.7 1,044,353 - PGA1_c10110 0.51 -1.1 1,044,390 + PGA1_c10110 0.55 -1.1 1,044,406 - PGA1_c10110 0.56 -0.4 1,044,416 - PGA1_c10110 0.57 +0.6 1,044,485 - PGA1_c10110 0.64 +1.2 1,044,733 + PGA1_c10110 0.89 -0.9 1,044,741 - PGA1_c10110 0.89 -1.2 1,044,802 + -2.4 1,044,811 + -0.8 1,044,816 + -2.2 1,044,824 - -1.2 1,044,846 - -0.3 1,044,850 + +0.7 1,044,877 + -0.4 1,044,877 + -1.0 1,044,895 - +0.3 1,044,977 - -0.3 1,045,016 - +1.2 1,045,055 - -0.3 1,045,077 - -0.4 1,045,096 + -2.2 1,045,140 + -1.0 1,045,198 + PGA1_c10120 0.12 -0.4 1,045,393 - PGA1_c10120 0.23 +0.5 1,045,408 + PGA1_c10120 0.24 +0.1 1,045,447 + PGA1_c10120 0.27 +0.6 1,045,523 - PGA1_c10120 0.31 -3.2 1,045,535 - PGA1_c10120 0.32 -0.8 1,045,535 - PGA1_c10120 0.32 +0.3 1,045,537 - PGA1_c10120 0.32 -1.7 1,045,549 + PGA1_c10120 0.33 -1.7 1,045,564 - PGA1_c10120 0.33 +1.9 1,045,726 + PGA1_c10120 0.43 -0.8 1,045,796 - PGA1_c10120 0.47 -0.7 1,045,801 - PGA1_c10120 0.47 -1.3 1,045,816 + PGA1_c10120 0.48 -1.4 1,045,816 + PGA1_c10120 0.48 -0.4 1,045,824 - PGA1_c10120 0.49 -0.2
Or see this region's nucleotide sequence