Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c07040 and PGA1_c07050 are separated by 87 nucleotides PGA1_c07050 and PGA1_c07060 are separated by 165 nucleotides PGA1_c07060 and PGA1_c07070 overlap by 4 nucleotides PGA1_c07070 and PGA1_c07080 are separated by 53 nucleotides
PGA1_c07040: PGA1_c07040 - transcriptional regulator, lysR family, at 724,131 to 725,009
_c07040
PGA1_c07050: PGA1_c07050 - methyltransferase domain-containing protein, at 725,097 to 725,732
_c07050
PGA1_c07060: PGA1_c07060 - YtoQ family protein, at 725,898 to 726,347
_c07060
PGA1_c07070: PGA1_c07070 - acetyltransferase domain-containing protein, at 726,344 to 726,871
_c07070
PGA1_c07080: PGA1_c07080 - small-conductance mechanosensitive channel, at 726,925 to 727,731
_c07080
Position (kb) 725
726
727 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 724.910 kb on - strand, within PGA1_c07040 at 724.912 kb on + strand, within PGA1_c07040 at 724.915 kb on - strand, within PGA1_c07040 at 724.918 kb on + strand, within PGA1_c07040 at 724.922 kb on + strand at 724.922 kb on + strand at 724.922 kb on + strand at 724.930 kb on - strand at 724.930 kb on - strand at 724.937 kb on + strand at 724.937 kb on + strand at 724.941 kb on + strand at 724.947 kb on + strand at 724.978 kb on + strand at 725.147 kb on + strand at 725.192 kb on + strand, within PGA1_c07050 at 725.197 kb on + strand, within PGA1_c07050 at 725.200 kb on - strand, within PGA1_c07050 at 725.200 kb on - strand, within PGA1_c07050 at 725.200 kb on - strand, within PGA1_c07050 at 725.205 kb on - strand, within PGA1_c07050 at 725.211 kb on - strand, within PGA1_c07050 at 725.219 kb on - strand, within PGA1_c07050 at 725.275 kb on - strand, within PGA1_c07050 at 725.325 kb on + strand, within PGA1_c07050 at 725.330 kb on + strand, within PGA1_c07050 at 725.338 kb on - strand, within PGA1_c07050 at 725.373 kb on - strand, within PGA1_c07050 at 725.390 kb on - strand, within PGA1_c07050 at 725.422 kb on + strand, within PGA1_c07050 at 725.430 kb on - strand, within PGA1_c07050 at 725.489 kb on - strand, within PGA1_c07050 at 725.532 kb on + strand, within PGA1_c07050 at 725.597 kb on + strand, within PGA1_c07050 at 725.751 kb on + strand at 725.769 kb on - strand at 725.769 kb on - strand at 725.793 kb on - strand at 725.802 kb on + strand at 725.836 kb on + strand at 725.867 kb on + strand at 725.954 kb on - strand, within PGA1_c07060 at 725.984 kb on + strand, within PGA1_c07060 at 725.992 kb on - strand, within PGA1_c07060 at 726.086 kb on + strand, within PGA1_c07060 at 726.089 kb on - strand, within PGA1_c07060 at 726.094 kb on - strand, within PGA1_c07060 at 726.111 kb on - strand, within PGA1_c07060 at 726.154 kb on + strand, within PGA1_c07060 at 726.201 kb on - strand, within PGA1_c07060 at 726.217 kb on - strand, within PGA1_c07060 at 726.224 kb on + strand, within PGA1_c07060 at 726.232 kb on - strand, within PGA1_c07060 at 726.440 kb on - strand, within PGA1_c07070 at 726.448 kb on + strand, within PGA1_c07070 at 726.479 kb on - strand, within PGA1_c07070 at 726.481 kb on + strand, within PGA1_c07070 at 726.481 kb on + strand, within PGA1_c07070 at 726.521 kb on + strand, within PGA1_c07070 at 726.549 kb on + strand, within PGA1_c07070 at 726.549 kb on + strand, within PGA1_c07070 at 726.647 kb on + strand, within PGA1_c07070 at 726.668 kb on + strand, within PGA1_c07070 at 726.674 kb on + strand, within PGA1_c07070 at 726.698 kb on - strand, within PGA1_c07070 at 726.801 kb on - strand, within PGA1_c07070 at 726.801 kb on - strand, within PGA1_c07070 at 726.989 kb on - strand at 727.026 kb on + strand, within PGA1_c07080 at 727.040 kb on + strand, within PGA1_c07080 at 727.048 kb on - strand, within PGA1_c07080 at 727.095 kb on + strand, within PGA1_c07080 at 727.227 kb on - strand, within PGA1_c07080 at 727.253 kb on + strand, within PGA1_c07080 at 727.253 kb on + strand, within PGA1_c07080 at 727.253 kb on + strand, within PGA1_c07080 at 727.253 kb on + strand, within PGA1_c07080 at 727.253 kb on + strand, within PGA1_c07080 at 727.261 kb on - strand, within PGA1_c07080 at 727.261 kb on - strand, within PGA1_c07080 at 727.261 kb on - strand, within PGA1_c07080 at 727.295 kb on + strand, within PGA1_c07080 at 727.328 kb on - strand, within PGA1_c07080
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 724,910 - PGA1_c07040 0.89 +1.8 724,912 + PGA1_c07040 0.89 +0.0 724,915 - PGA1_c07040 0.89 +0.9 724,918 + PGA1_c07040 0.90 +1.0 724,922 + +0.9 724,922 + +0.1 724,922 + -0.2 724,930 - -1.2 724,930 - +3.1 724,937 + -0.7 724,937 + -0.8 724,941 + +0.6 724,947 + -0.5 724,978 + -1.6 725,147 + -0.3 725,192 + PGA1_c07050 0.15 -0.9 725,197 + PGA1_c07050 0.16 -0.3 725,200 - PGA1_c07050 0.16 +0.0 725,200 - PGA1_c07050 0.16 +0.5 725,200 - PGA1_c07050 0.16 +0.5 725,205 - PGA1_c07050 0.17 -0.1 725,211 - PGA1_c07050 0.18 -0.6 725,219 - PGA1_c07050 0.19 -0.5 725,275 - PGA1_c07050 0.28 +0.7 725,325 + PGA1_c07050 0.36 +0.2 725,330 + PGA1_c07050 0.37 +0.7 725,338 - PGA1_c07050 0.38 -2.0 725,373 - PGA1_c07050 0.43 -0.0 725,390 - PGA1_c07050 0.46 +0.7 725,422 + PGA1_c07050 0.51 -1.8 725,430 - PGA1_c07050 0.52 -0.6 725,489 - PGA1_c07050 0.62 +0.8 725,532 + PGA1_c07050 0.68 +0.4 725,597 + PGA1_c07050 0.79 -0.7 725,751 + +0.4 725,769 - +0.1 725,769 - -0.8 725,793 - +0.8 725,802 + +0.1 725,836 + +0.7 725,867 + -0.5 725,954 - PGA1_c07060 0.12 +0.7 725,984 + PGA1_c07060 0.19 +0.9 725,992 - PGA1_c07060 0.21 +1.5 726,086 + PGA1_c07060 0.42 -0.9 726,089 - PGA1_c07060 0.42 -0.5 726,094 - PGA1_c07060 0.44 +0.3 726,111 - PGA1_c07060 0.47 +0.5 726,154 + PGA1_c07060 0.57 -0.2 726,201 - PGA1_c07060 0.67 -0.5 726,217 - PGA1_c07060 0.71 +0.9 726,224 + PGA1_c07060 0.72 +0.0 726,232 - PGA1_c07060 0.74 +1.0 726,440 - PGA1_c07070 0.18 +0.1 726,448 + PGA1_c07070 0.20 +1.1 726,479 - PGA1_c07070 0.26 +0.2 726,481 + PGA1_c07070 0.26 +0.8 726,481 + PGA1_c07070 0.26 -0.4 726,521 + PGA1_c07070 0.34 +0.4 726,549 + PGA1_c07070 0.39 -0.5 726,549 + PGA1_c07070 0.39 +0.3 726,647 + PGA1_c07070 0.57 +1.1 726,668 + PGA1_c07070 0.61 +0.4 726,674 + PGA1_c07070 0.62 +0.5 726,698 - PGA1_c07070 0.67 +1.5 726,801 - PGA1_c07070 0.87 -0.2 726,801 - PGA1_c07070 0.87 +0.4 726,989 - +0.1 727,026 + PGA1_c07080 0.13 -0.3 727,040 + PGA1_c07080 0.14 -0.6 727,048 - PGA1_c07080 0.15 +0.1 727,095 + PGA1_c07080 0.21 +0.0 727,227 - PGA1_c07080 0.37 +1.9 727,253 + PGA1_c07080 0.41 +0.3 727,253 + PGA1_c07080 0.41 +1.3 727,253 + PGA1_c07080 0.41 +0.8 727,253 + PGA1_c07080 0.41 -1.9 727,253 + PGA1_c07080 0.41 +0.4 727,261 - PGA1_c07080 0.42 -1.6 727,261 - PGA1_c07080 0.42 -0.3 727,261 - PGA1_c07080 0.42 -0.9 727,295 + PGA1_c07080 0.46 +0.6 727,328 - PGA1_c07080 0.50 +0.8
Or see this region's nucleotide sequence