Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c31530 and PGA1_c31540 are separated by 8 nucleotides PGA1_c31540 and PGA1_c31550 are separated by 37 nucleotides PGA1_c31550 and PGA1_c31560 are separated by 29 nucleotides
PGA1_c31530: PGA1_c31530 - putative cytosol aminopeptidase, at 3,294,483 to 3,295,877
_c31530
PGA1_c31540: PGA1_c31540 - Cell wall-associated hydrolases (invasion-associated proteins), at 3,295,886 to 3,296,734
_c31540
PGA1_c31550: PGA1_c31550 - Protein of unknown function (DUF2794)., at 3,296,772 to 3,297,155
_c31550
PGA1_c31560: PGA1_c31560 - putative HTH-type transcriptional regulator, ArsR family, at 3,297,185 to 3,297,892
_c31560
Position (kb)
3295
3296
3297 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3294.887 kb on - strand, within PGA1_c31530 at 3294.887 kb on - strand, within PGA1_c31530 at 3294.914 kb on + strand, within PGA1_c31530 at 3294.922 kb on - strand, within PGA1_c31530 at 3294.946 kb on + strand, within PGA1_c31530 at 3294.954 kb on - strand, within PGA1_c31530 at 3294.980 kb on - strand, within PGA1_c31530 at 3295.013 kb on - strand, within PGA1_c31530 at 3295.013 kb on - strand, within PGA1_c31530 at 3295.018 kb on - strand, within PGA1_c31530 at 3295.018 kb on - strand, within PGA1_c31530 at 3295.018 kb on - strand, within PGA1_c31530 at 3295.025 kb on + strand, within PGA1_c31530 at 3295.036 kb on + strand, within PGA1_c31530 at 3295.036 kb on + strand, within PGA1_c31530 at 3295.036 kb on + strand, within PGA1_c31530 at 3295.036 kb on + strand, within PGA1_c31530 at 3295.038 kb on - strand, within PGA1_c31530 at 3295.044 kb on - strand, within PGA1_c31530 at 3295.044 kb on - strand, within PGA1_c31530 at 3295.044 kb on - strand, within PGA1_c31530 at 3295.044 kb on - strand, within PGA1_c31530 at 3295.044 kb on - strand, within PGA1_c31530 at 3295.085 kb on + strand, within PGA1_c31530 at 3295.142 kb on + strand, within PGA1_c31530 at 3295.142 kb on + strand, within PGA1_c31530 at 3295.150 kb on - strand, within PGA1_c31530 at 3295.150 kb on - strand, within PGA1_c31530 at 3295.150 kb on - strand, within PGA1_c31530 at 3295.168 kb on - strand, within PGA1_c31530 at 3295.225 kb on + strand, within PGA1_c31530 at 3295.225 kb on + strand, within PGA1_c31530 at 3295.244 kb on + strand, within PGA1_c31530 at 3295.272 kb on + strand, within PGA1_c31530 at 3295.335 kb on + strand, within PGA1_c31530 at 3295.440 kb on + strand, within PGA1_c31530 at 3295.452 kb on + strand, within PGA1_c31530 at 3295.457 kb on + strand, within PGA1_c31530 at 3295.457 kb on + strand, within PGA1_c31530 at 3295.457 kb on + strand, within PGA1_c31530 at 3295.465 kb on - strand, within PGA1_c31530 at 3295.470 kb on - strand, within PGA1_c31530 at 3295.480 kb on - strand, within PGA1_c31530 at 3295.512 kb on + strand, within PGA1_c31530 at 3295.520 kb on - strand, within PGA1_c31530 at 3295.520 kb on - strand, within PGA1_c31530 at 3295.558 kb on - strand, within PGA1_c31530 at 3295.617 kb on + strand, within PGA1_c31530 at 3295.630 kb on - strand, within PGA1_c31530 at 3295.651 kb on - strand, within PGA1_c31530 at 3295.662 kb on + strand, within PGA1_c31530 at 3295.722 kb on + strand, within PGA1_c31530 at 3295.792 kb on + strand at 3295.797 kb on + strand at 3295.805 kb on - strand at 3295.805 kb on - strand at 3296.002 kb on + strand, within PGA1_c31540 at 3296.011 kb on + strand, within PGA1_c31540 at 3296.055 kb on + strand, within PGA1_c31540 at 3296.089 kb on - strand, within PGA1_c31540 at 3296.095 kb on - strand, within PGA1_c31540 at 3296.098 kb on + strand, within PGA1_c31540 at 3296.102 kb on - strand, within PGA1_c31540 at 3296.104 kb on + strand, within PGA1_c31540 at 3296.106 kb on - strand, within PGA1_c31540 at 3296.116 kb on - strand, within PGA1_c31540 at 3296.132 kb on + strand, within PGA1_c31540 at 3296.140 kb on - strand, within PGA1_c31540 at 3296.157 kb on + strand, within PGA1_c31540 at 3296.174 kb on + strand, within PGA1_c31540 at 3296.179 kb on + strand, within PGA1_c31540 at 3296.182 kb on - strand, within PGA1_c31540 at 3296.267 kb on + strand, within PGA1_c31540 at 3296.267 kb on + strand, within PGA1_c31540 at 3296.300 kb on + strand, within PGA1_c31540 at 3296.313 kb on - strand, within PGA1_c31540 at 3296.318 kb on - strand, within PGA1_c31540 at 3296.364 kb on + strand, within PGA1_c31540 at 3296.372 kb on - strand, within PGA1_c31540 at 3296.388 kb on + strand, within PGA1_c31540 at 3296.388 kb on - strand, within PGA1_c31540 at 3296.396 kb on - strand, within PGA1_c31540 at 3296.430 kb on + strand, within PGA1_c31540 at 3296.440 kb on + strand, within PGA1_c31540 at 3296.478 kb on - strand, within PGA1_c31540 at 3296.479 kb on + strand, within PGA1_c31540 at 3296.483 kb on - strand, within PGA1_c31540 at 3296.483 kb on - strand, within PGA1_c31540 at 3296.535 kb on - strand, within PGA1_c31540 at 3296.552 kb on - strand, within PGA1_c31540 at 3296.612 kb on + strand, within PGA1_c31540 at 3296.612 kb on + strand, within PGA1_c31540 at 3296.612 kb on + strand, within PGA1_c31540 at 3296.620 kb on - strand, within PGA1_c31540 at 3296.688 kb on - strand at 3296.739 kb on - strand at 3296.739 kb on - strand at 3296.775 kb on + strand at 3296.782 kb on + strand at 3296.792 kb on + strand at 3296.813 kb on + strand, within PGA1_c31550 at 3296.844 kb on + strand, within PGA1_c31550 at 3296.902 kb on - strand, within PGA1_c31550 at 3296.902 kb on - strand, within PGA1_c31550 at 3296.927 kb on + strand, within PGA1_c31550 at 3296.927 kb on + strand, within PGA1_c31550 at 3297.019 kb on + strand, within PGA1_c31550 at 3297.019 kb on + strand, within PGA1_c31550 at 3297.054 kb on + strand, within PGA1_c31550 at 3297.062 kb on - strand, within PGA1_c31550 at 3297.062 kb on - strand, within PGA1_c31550 at 3297.114 kb on + strand, within PGA1_c31550 at 3297.119 kb on - strand at 3297.208 kb on + strand at 3297.223 kb on - strand at 3297.236 kb on - strand at 3297.282 kb on - strand, within PGA1_c31560 at 3297.331 kb on + strand, within PGA1_c31560 at 3297.375 kb on - strand, within PGA1_c31560 at 3297.381 kb on + strand, within PGA1_c31560 at 3297.385 kb on - strand, within PGA1_c31560 at 3297.389 kb on - strand, within PGA1_c31560 at 3297.424 kb on + strand, within PGA1_c31560 at 3297.425 kb on + strand, within PGA1_c31560 at 3297.428 kb on - strand, within PGA1_c31560 at 3297.430 kb on + strand, within PGA1_c31560 at 3297.430 kb on + strand, within PGA1_c31560 at 3297.430 kb on + strand, within PGA1_c31560 at 3297.430 kb on + strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.438 kb on - strand, within PGA1_c31560 at 3297.463 kb on + strand, within PGA1_c31560 at 3297.463 kb on + strand, within PGA1_c31560 at 3297.463 kb on + strand, within PGA1_c31560 at 3297.471 kb on - strand, within PGA1_c31560 at 3297.474 kb on + strand, within PGA1_c31560 at 3297.494 kb on + strand, within PGA1_c31560 at 3297.547 kb on + strand, within PGA1_c31560 at 3297.565 kb on - strand, within PGA1_c31560 at 3297.637 kb on + strand, within PGA1_c31560 at 3297.645 kb on - strand, within PGA1_c31560 at 3297.653 kb on - strand, within PGA1_c31560 at 3297.655 kb on + strand, within PGA1_c31560 at 3297.656 kb on - strand, within PGA1_c31560 at 3297.663 kb on - strand, within PGA1_c31560 at 3297.663 kb on - strand, within PGA1_c31560 at 3297.682 kb on - strand, within PGA1_c31560 at 3297.707 kb on + strand, within PGA1_c31560 at 3297.714 kb on + strand, within PGA1_c31560 at 3297.715 kb on - strand, within PGA1_c31560 at 3297.720 kb on - strand, within PGA1_c31560
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 3,294,887 - PGA1_c31530 0.29 -1.1 3,294,887 - PGA1_c31530 0.29 -1.4 3,294,914 + PGA1_c31530 0.31 -0.5 3,294,922 - PGA1_c31530 0.31 +1.7 3,294,946 + PGA1_c31530 0.33 -1.1 3,294,954 - PGA1_c31530 0.34 -1.3 3,294,980 - PGA1_c31530 0.36 +0.3 3,295,013 - PGA1_c31530 0.38 +1.2 3,295,013 - PGA1_c31530 0.38 +1.1 3,295,018 - PGA1_c31530 0.38 -0.4 3,295,018 - PGA1_c31530 0.38 +0.8 3,295,018 - PGA1_c31530 0.38 -0.9 3,295,025 + PGA1_c31530 0.39 +1.4 3,295,036 + PGA1_c31530 0.40 +0.9 3,295,036 + PGA1_c31530 0.40 -0.8 3,295,036 + PGA1_c31530 0.40 +1.5 3,295,036 + PGA1_c31530 0.40 -1.4 3,295,038 - PGA1_c31530 0.40 -0.1 3,295,044 - PGA1_c31530 0.40 +1.2 3,295,044 - PGA1_c31530 0.40 +0.2 3,295,044 - PGA1_c31530 0.40 +0.6 3,295,044 - PGA1_c31530 0.40 +1.0 3,295,044 - PGA1_c31530 0.40 -0.6 3,295,085 + PGA1_c31530 0.43 -1.1 3,295,142 + PGA1_c31530 0.47 +1.2 3,295,142 + PGA1_c31530 0.47 -2.0 3,295,150 - PGA1_c31530 0.48 -1.8 3,295,150 - PGA1_c31530 0.48 +1.6 3,295,150 - PGA1_c31530 0.48 -1.0 3,295,168 - PGA1_c31530 0.49 +0.3 3,295,225 + PGA1_c31530 0.53 +2.5 3,295,225 + PGA1_c31530 0.53 +0.5 3,295,244 + PGA1_c31530 0.55 -1.0 3,295,272 + PGA1_c31530 0.57 +1.2 3,295,335 + PGA1_c31530 0.61 -0.8 3,295,440 + PGA1_c31530 0.69 +1.1 3,295,452 + PGA1_c31530 0.69 +0.8 3,295,457 + PGA1_c31530 0.70 +0.2 3,295,457 + PGA1_c31530 0.70 -0.0 3,295,457 + PGA1_c31530 0.70 -0.0 3,295,465 - PGA1_c31530 0.70 +0.9 3,295,470 - PGA1_c31530 0.71 -1.4 3,295,480 - PGA1_c31530 0.71 +1.2 3,295,512 + PGA1_c31530 0.74 +0.9 3,295,520 - PGA1_c31530 0.74 +0.7 3,295,520 - PGA1_c31530 0.74 -1.1 3,295,558 - PGA1_c31530 0.77 +0.4 3,295,617 + PGA1_c31530 0.81 -0.2 3,295,630 - PGA1_c31530 0.82 +1.2 3,295,651 - PGA1_c31530 0.84 -0.7 3,295,662 + PGA1_c31530 0.85 +0.6 3,295,722 + PGA1_c31530 0.89 +1.2 3,295,792 + +0.0 3,295,797 + -0.1 3,295,805 - -0.1 3,295,805 - -1.1 3,296,002 + PGA1_c31540 0.14 -1.5 3,296,011 + PGA1_c31540 0.15 -1.1 3,296,055 + PGA1_c31540 0.20 +1.2 3,296,089 - PGA1_c31540 0.24 -1.0 3,296,095 - PGA1_c31540 0.25 -0.3 3,296,098 + PGA1_c31540 0.25 +0.2 3,296,102 - PGA1_c31540 0.25 -0.0 3,296,104 + PGA1_c31540 0.26 -0.5 3,296,106 - PGA1_c31540 0.26 +0.6 3,296,116 - PGA1_c31540 0.27 -0.7 3,296,132 + PGA1_c31540 0.29 -0.4 3,296,140 - PGA1_c31540 0.30 +0.2 3,296,157 + PGA1_c31540 0.32 -0.2 3,296,174 + PGA1_c31540 0.34 -0.4 3,296,179 + PGA1_c31540 0.35 +0.2 3,296,182 - PGA1_c31540 0.35 -0.7 3,296,267 + PGA1_c31540 0.45 -2.7 3,296,267 + PGA1_c31540 0.45 -2.0 3,296,300 + PGA1_c31540 0.49 -0.7 3,296,313 - PGA1_c31540 0.50 -0.5 3,296,318 - PGA1_c31540 0.51 -0.2 3,296,364 + PGA1_c31540 0.56 -2.0 3,296,372 - PGA1_c31540 0.57 +0.8 3,296,388 + PGA1_c31540 0.59 -0.4 3,296,388 - PGA1_c31540 0.59 -0.2 3,296,396 - PGA1_c31540 0.60 +0.4 3,296,430 + PGA1_c31540 0.64 +0.2 3,296,440 + PGA1_c31540 0.65 +0.4 3,296,478 - PGA1_c31540 0.70 +1.2 3,296,479 + PGA1_c31540 0.70 -0.4 3,296,483 - PGA1_c31540 0.70 +0.6 3,296,483 - PGA1_c31540 0.70 -0.6 3,296,535 - PGA1_c31540 0.76 -1.0 3,296,552 - PGA1_c31540 0.78 -0.4 3,296,612 + PGA1_c31540 0.86 -0.1 3,296,612 + PGA1_c31540 0.86 -1.2 3,296,612 + PGA1_c31540 0.86 -1.8 3,296,620 - PGA1_c31540 0.86 -0.7 3,296,688 - -2.1 3,296,739 - +0.4 3,296,739 - +0.4 3,296,775 + +0.4 3,296,782 + -0.5 3,296,792 + +0.9 3,296,813 + PGA1_c31550 0.11 -0.8 3,296,844 + PGA1_c31550 0.19 +0.2 3,296,902 - PGA1_c31550 0.34 +1.2 3,296,902 - PGA1_c31550 0.34 +0.5 3,296,927 + PGA1_c31550 0.40 +1.1 3,296,927 + PGA1_c31550 0.40 -1.4 3,297,019 + PGA1_c31550 0.64 -0.4 3,297,019 + PGA1_c31550 0.64 -0.0 3,297,054 + PGA1_c31550 0.73 +0.4 3,297,062 - PGA1_c31550 0.76 -0.2 3,297,062 - PGA1_c31550 0.76 +0.4 3,297,114 + PGA1_c31550 0.89 -0.2 3,297,119 - -1.8 3,297,208 + +0.5 3,297,223 - -1.4 3,297,236 - -0.2 3,297,282 - PGA1_c31560 0.14 +0.8 3,297,331 + PGA1_c31560 0.21 -0.5 3,297,375 - PGA1_c31560 0.27 -0.4 3,297,381 + PGA1_c31560 0.28 +2.2 3,297,385 - PGA1_c31560 0.28 +0.0 3,297,389 - PGA1_c31560 0.29 +0.2 3,297,424 + PGA1_c31560 0.34 +0.2 3,297,425 + PGA1_c31560 0.34 -0.7 3,297,428 - PGA1_c31560 0.34 +0.4 3,297,430 + PGA1_c31560 0.35 -0.7 3,297,430 + PGA1_c31560 0.35 -0.4 3,297,430 + PGA1_c31560 0.35 -1.0 3,297,430 + PGA1_c31560 0.35 +0.0 3,297,438 - PGA1_c31560 0.36 +1.2 3,297,438 - PGA1_c31560 0.36 -0.4 3,297,438 - PGA1_c31560 0.36 +1.2 3,297,438 - PGA1_c31560 0.36 -1.6 3,297,438 - PGA1_c31560 0.36 -0.0 3,297,438 - PGA1_c31560 0.36 +1.2 3,297,438 - PGA1_c31560 0.36 -0.9 3,297,438 - PGA1_c31560 0.36 -0.6 3,297,438 - PGA1_c31560 0.36 -0.1 3,297,438 - PGA1_c31560 0.36 -1.6 3,297,438 - PGA1_c31560 0.36 +2.5 3,297,463 + PGA1_c31560 0.39 -1.1 3,297,463 + PGA1_c31560 0.39 -0.1 3,297,463 + PGA1_c31560 0.39 +0.9 3,297,471 - PGA1_c31560 0.40 +0.0 3,297,474 + PGA1_c31560 0.41 +1.2 3,297,494 + PGA1_c31560 0.44 -1.1 3,297,547 + PGA1_c31560 0.51 -1.6 3,297,565 - PGA1_c31560 0.54 +0.9 3,297,637 + PGA1_c31560 0.64 +0.8 3,297,645 - PGA1_c31560 0.65 -0.7 3,297,653 - PGA1_c31560 0.66 +0.1 3,297,655 + PGA1_c31560 0.66 -1.0 3,297,656 - PGA1_c31560 0.67 -0.0 3,297,663 - PGA1_c31560 0.68 -2.2 3,297,663 - PGA1_c31560 0.68 -0.4 3,297,682 - PGA1_c31560 0.70 -1.7 3,297,707 + PGA1_c31560 0.74 -1.6 3,297,714 + PGA1_c31560 0.75 +0.6 3,297,715 - PGA1_c31560 0.75 -0.9 3,297,720 - PGA1_c31560 0.76 -1.0
Or see this region's nucleotide sequence