Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c29750 and PGA1_c29760 are separated by 84 nucleotides PGA1_c29760 and PGA1_c29770 are separated by 229 nucleotides PGA1_c29770 and PGA1_c29780 are separated by 306 nucleotides
PGA1_c29750: PGA1_c29750 - putative ribosomal RNA large subunit methyltransferase, at 3,111,775 to 3,112,245
_c29750
PGA1_c29760: PGA1_c29760 - iojap-like ribosome-associated protein, at 3,112,330 to 3,112,698
_c29760
PGA1_c29770: PGA1_c29770 - putative mechanosensitive ion channel, at 3,112,928 to 3,114,289
_c29770
PGA1_c29780: PGA1_c29780 - 3-isopropylmalate dehydratase large subunit, at 3,114,596 to 3,115,999
_c29780
Position (kb)
3112
3113
3114
3115 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3111.954 kb on - strand, within PGA1_c29750 at 3111.954 kb on - strand, within PGA1_c29750 at 3111.954 kb on - strand, within PGA1_c29750 at 3112.026 kb on + strand, within PGA1_c29750 at 3112.026 kb on + strand, within PGA1_c29750 at 3112.039 kb on + strand, within PGA1_c29750 at 3112.040 kb on - strand, within PGA1_c29750 at 3112.056 kb on + strand, within PGA1_c29750 at 3112.069 kb on - strand, within PGA1_c29750 at 3112.142 kb on - strand, within PGA1_c29750 at 3112.142 kb on - strand, within PGA1_c29750 at 3112.142 kb on - strand, within PGA1_c29750 at 3112.207 kb on + strand at 3112.208 kb on + strand at 3112.275 kb on + strand at 3112.295 kb on - strand at 3112.394 kb on - strand, within PGA1_c29760 at 3112.409 kb on - strand, within PGA1_c29760 at 3112.414 kb on - strand, within PGA1_c29760 at 3112.469 kb on + strand, within PGA1_c29760 at 3112.476 kb on - strand, within PGA1_c29760 at 3112.561 kb on + strand, within PGA1_c29760 at 3112.562 kb on + strand, within PGA1_c29760 at 3112.673 kb on - strand at 3112.750 kb on - strand at 3112.787 kb on + strand at 3112.799 kb on + strand at 3112.836 kb on + strand at 3112.851 kb on + strand at 3112.863 kb on + strand at 3112.868 kb on + strand at 3112.868 kb on + strand at 3112.876 kb on - strand at 3112.876 kb on - strand at 3112.883 kb on - strand at 3112.904 kb on - strand at 3112.905 kb on + strand at 3112.941 kb on + strand at 3112.941 kb on + strand at 3112.947 kb on + strand at 3113.008 kb on + strand at 3113.015 kb on - strand at 3113.051 kb on - strand at 3113.094 kb on + strand, within PGA1_c29770 at 3113.094 kb on + strand, within PGA1_c29770 at 3113.106 kb on + strand, within PGA1_c29770 at 3113.108 kb on + strand, within PGA1_c29770 at 3113.175 kb on + strand, within PGA1_c29770 at 3113.176 kb on - strand, within PGA1_c29770 at 3113.215 kb on + strand, within PGA1_c29770 at 3113.215 kb on + strand, within PGA1_c29770 at 3113.287 kb on + strand, within PGA1_c29770 at 3113.287 kb on + strand, within PGA1_c29770 at 3113.332 kb on + strand, within PGA1_c29770 at 3113.340 kb on - strand, within PGA1_c29770 at 3113.340 kb on - strand, within PGA1_c29770 at 3113.387 kb on + strand, within PGA1_c29770 at 3113.415 kb on + strand, within PGA1_c29770 at 3113.424 kb on + strand, within PGA1_c29770 at 3113.477 kb on - strand, within PGA1_c29770 at 3113.505 kb on + strand, within PGA1_c29770 at 3113.513 kb on - strand, within PGA1_c29770 at 3113.585 kb on + strand, within PGA1_c29770 at 3113.721 kb on + strand, within PGA1_c29770 at 3113.727 kb on + strand, within PGA1_c29770 at 3113.729 kb on - strand, within PGA1_c29770 at 3113.744 kb on - strand, within PGA1_c29770 at 3113.776 kb on - strand, within PGA1_c29770 at 3113.812 kb on + strand, within PGA1_c29770 at 3113.820 kb on - strand, within PGA1_c29770 at 3113.820 kb on - strand, within PGA1_c29770 at 3113.820 kb on - strand, within PGA1_c29770 at 3113.820 kb on - strand, within PGA1_c29770 at 3113.890 kb on + strand, within PGA1_c29770 at 3113.935 kb on - strand, within PGA1_c29770 at 3113.952 kb on + strand, within PGA1_c29770 at 3113.960 kb on - strand, within PGA1_c29770 at 3113.960 kb on - strand, within PGA1_c29770 at 3113.987 kb on - strand, within PGA1_c29770 at 3113.987 kb on - strand, within PGA1_c29770 at 3114.033 kb on - strand, within PGA1_c29770 at 3114.033 kb on - strand, within PGA1_c29770 at 3114.033 kb on - strand, within PGA1_c29770 at 3114.040 kb on + strand, within PGA1_c29770 at 3114.053 kb on - strand, within PGA1_c29770 at 3114.080 kb on + strand, within PGA1_c29770 at 3114.115 kb on - strand, within PGA1_c29770 at 3114.115 kb on - strand, within PGA1_c29770 at 3114.128 kb on + strand, within PGA1_c29770 at 3114.128 kb on - strand, within PGA1_c29770 at 3114.136 kb on - strand, within PGA1_c29770 at 3114.152 kb on - strand, within PGA1_c29770 at 3114.243 kb on + strand at 3114.243 kb on + strand at 3114.243 kb on + strand at 3114.264 kb on + strand at 3114.264 kb on + strand at 3114.264 kb on + strand at 3114.297 kb on + strand at 3114.358 kb on + strand at 3114.358 kb on + strand at 3114.365 kb on + strand at 3114.365 kb on + strand at 3114.365 kb on + strand at 3114.366 kb on - strand at 3114.373 kb on - strand at 3114.386 kb on - strand at 3114.523 kb on + strand at 3114.523 kb on + strand at 3114.523 kb on + strand at 3114.525 kb on + strand at 3114.531 kb on - strand at 3114.534 kb on + strand at 3114.588 kb on - strand at 3114.590 kb on + strand at 3114.590 kb on + strand at 3114.598 kb on - strand at 3114.598 kb on - strand at 3114.716 kb on - strand at 3114.716 kb on - strand at 3114.738 kb on - strand, within PGA1_c29780 at 3114.801 kb on + strand, within PGA1_c29780 at 3114.837 kb on + strand, within PGA1_c29780 at 3114.946 kb on + strand, within PGA1_c29780 at 3114.946 kb on + strand, within PGA1_c29780 at 3114.948 kb on + strand, within PGA1_c29780 at 3114.949 kb on - strand, within PGA1_c29780 at 3114.949 kb on - strand, within PGA1_c29780 at 3114.954 kb on - strand, within PGA1_c29780 at 3114.971 kb on - strand, within PGA1_c29780 at 3114.985 kb on + strand, within PGA1_c29780 at 3115.038 kb on + strand, within PGA1_c29780 at 3115.038 kb on + strand, within PGA1_c29780 at 3115.065 kb on + strand, within PGA1_c29780 at 3115.071 kb on + strand, within PGA1_c29780 at 3115.152 kb on - strand, within PGA1_c29780 at 3115.187 kb on + strand, within PGA1_c29780 at 3115.256 kb on + strand, within PGA1_c29780 at 3115.256 kb on - strand, within PGA1_c29780
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 3,111,954 - PGA1_c29750 0.38 -0.2 3,111,954 - PGA1_c29750 0.38 +0.5 3,111,954 - PGA1_c29750 0.38 -0.5 3,112,026 + PGA1_c29750 0.53 -1.1 3,112,026 + PGA1_c29750 0.53 +0.4 3,112,039 + PGA1_c29750 0.56 +0.8 3,112,040 - PGA1_c29750 0.56 -0.2 3,112,056 + PGA1_c29750 0.60 -0.6 3,112,069 - PGA1_c29750 0.62 -1.3 3,112,142 - PGA1_c29750 0.78 -0.7 3,112,142 - PGA1_c29750 0.78 -0.1 3,112,142 - PGA1_c29750 0.78 +0.7 3,112,207 + -0.8 3,112,208 + -1.4 3,112,275 + +0.8 3,112,295 - -1.0 3,112,394 - PGA1_c29760 0.17 +1.6 3,112,409 - PGA1_c29760 0.21 -0.7 3,112,414 - PGA1_c29760 0.23 -0.1 3,112,469 + PGA1_c29760 0.38 +1.6 3,112,476 - PGA1_c29760 0.40 +0.4 3,112,561 + PGA1_c29760 0.63 +0.9 3,112,562 + PGA1_c29760 0.63 +0.0 3,112,673 - -1.0 3,112,750 - +1.5 3,112,787 + +1.0 3,112,799 + +0.4 3,112,836 + -0.2 3,112,851 + +1.2 3,112,863 + +1.4 3,112,868 + +0.5 3,112,868 + -2.6 3,112,876 - +0.3 3,112,876 - +1.2 3,112,883 - +1.2 3,112,904 - -0.1 3,112,905 + -1.1 3,112,941 + -1.8 3,112,941 + +0.5 3,112,947 + +0.2 3,113,008 + -0.1 3,113,015 - -0.0 3,113,051 - -1.6 3,113,094 + PGA1_c29770 0.12 -0.8 3,113,094 + PGA1_c29770 0.12 +0.8 3,113,106 + PGA1_c29770 0.13 +0.4 3,113,108 + PGA1_c29770 0.13 +1.4 3,113,175 + PGA1_c29770 0.18 -2.8 3,113,176 - PGA1_c29770 0.18 +0.0 3,113,215 + PGA1_c29770 0.21 +1.8 3,113,215 + PGA1_c29770 0.21 +0.6 3,113,287 + PGA1_c29770 0.26 +0.2 3,113,287 + PGA1_c29770 0.26 +2.2 3,113,332 + PGA1_c29770 0.30 -1.8 3,113,340 - PGA1_c29770 0.30 -0.8 3,113,340 - PGA1_c29770 0.30 +0.3 3,113,387 + PGA1_c29770 0.34 -0.0 3,113,415 + PGA1_c29770 0.36 -1.0 3,113,424 + PGA1_c29770 0.36 +1.5 3,113,477 - PGA1_c29770 0.40 +1.0 3,113,505 + PGA1_c29770 0.42 +1.1 3,113,513 - PGA1_c29770 0.43 +1.4 3,113,585 + PGA1_c29770 0.48 -0.8 3,113,721 + PGA1_c29770 0.58 +1.2 3,113,727 + PGA1_c29770 0.59 +0.5 3,113,729 - PGA1_c29770 0.59 +0.6 3,113,744 - PGA1_c29770 0.60 -1.4 3,113,776 - PGA1_c29770 0.62 -0.5 3,113,812 + PGA1_c29770 0.65 -1.8 3,113,820 - PGA1_c29770 0.65 +1.4 3,113,820 - PGA1_c29770 0.65 -0.7 3,113,820 - PGA1_c29770 0.65 -0.5 3,113,820 - PGA1_c29770 0.65 -0.8 3,113,890 + PGA1_c29770 0.71 +2.2 3,113,935 - PGA1_c29770 0.74 +1.9 3,113,952 + PGA1_c29770 0.75 -1.4 3,113,960 - PGA1_c29770 0.76 -0.9 3,113,960 - PGA1_c29770 0.76 -0.8 3,113,987 - PGA1_c29770 0.78 +0.4 3,113,987 - PGA1_c29770 0.78 +1.4 3,114,033 - PGA1_c29770 0.81 -0.9 3,114,033 - PGA1_c29770 0.81 -0.4 3,114,033 - PGA1_c29770 0.81 +2.7 3,114,040 + PGA1_c29770 0.82 +0.3 3,114,053 - PGA1_c29770 0.83 -2.4 3,114,080 + PGA1_c29770 0.85 +1.7 3,114,115 - PGA1_c29770 0.87 -1.7 3,114,115 - PGA1_c29770 0.87 +0.3 3,114,128 + PGA1_c29770 0.88 -0.8 3,114,128 - PGA1_c29770 0.88 +0.5 3,114,136 - PGA1_c29770 0.89 +1.2 3,114,152 - PGA1_c29770 0.90 -0.2 3,114,243 + -1.6 3,114,243 + +0.1 3,114,243 + +2.4 3,114,264 + -0.8 3,114,264 + +1.2 3,114,264 + +0.1 3,114,297 + -0.0 3,114,358 + +0.6 3,114,358 + +0.6 3,114,365 + -1.4 3,114,365 + +0.3 3,114,365 + +1.8 3,114,366 - -1.7 3,114,373 - -0.3 3,114,386 - +1.9 3,114,523 + -0.6 3,114,523 + -3.0 3,114,523 + +1.4 3,114,525 + -0.4 3,114,531 - -2.7 3,114,534 + -0.0 3,114,588 - -2.8 3,114,590 + -2.0 3,114,590 + -1.4 3,114,598 - -2.0 3,114,598 - +0.2 3,114,716 - +0.2 3,114,716 - -2.1 3,114,738 - PGA1_c29780 0.10 -3.3 3,114,801 + PGA1_c29780 0.15 -3.0 3,114,837 + PGA1_c29780 0.17 -2.3 3,114,946 + PGA1_c29780 0.25 -1.7 3,114,946 + PGA1_c29780 0.25 -0.8 3,114,948 + PGA1_c29780 0.25 -2.8 3,114,949 - PGA1_c29780 0.25 +0.2 3,114,949 - PGA1_c29780 0.25 -0.8 3,114,954 - PGA1_c29780 0.25 -2.3 3,114,971 - PGA1_c29780 0.27 -2.0 3,114,985 + PGA1_c29780 0.28 -1.1 3,115,038 + PGA1_c29780 0.31 -2.3 3,115,038 + PGA1_c29780 0.31 -1.8 3,115,065 + PGA1_c29780 0.33 -2.3 3,115,071 + PGA1_c29780 0.34 -3.0 3,115,152 - PGA1_c29780 0.40 -2.0 3,115,187 + PGA1_c29780 0.42 -3.3 3,115,256 + PGA1_c29780 0.47 -2.2 3,115,256 - PGA1_c29780 0.47 -0.8
Or see this region's nucleotide sequence