Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12350 and PGA1_c12360 are separated by 81 nucleotides PGA1_c12360 and PGA1_c12370 are separated by 103 nucleotides
PGA1_c12350: PGA1_c12350 - acetolactate synthase, catabolic, at 1,284,241 to 1,285,905
_c12350
PGA1_c12360: PGA1_c12360 - Uncharacterized conserved protein, at 1,285,987 to 1,286,526
_c12360
PGA1_c12370: PGA1_c12370 - YeeE/YedE family (DUF395)., at 1,286,630 to 1,287,700
_c12370
Position (kb)
1285
1286
1287 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1285.044 kb on + strand, within PGA1_c12350 at 1285.127 kb on - strand, within PGA1_c12350 at 1285.171 kb on - strand, within PGA1_c12350 at 1285.256 kb on - strand, within PGA1_c12350 at 1285.306 kb on + strand, within PGA1_c12350 at 1285.329 kb on + strand, within PGA1_c12350 at 1285.334 kb on + strand, within PGA1_c12350 at 1285.402 kb on + strand, within PGA1_c12350 at 1285.438 kb on + strand, within PGA1_c12350 at 1285.438 kb on + strand, within PGA1_c12350 at 1285.446 kb on - strand, within PGA1_c12350 at 1285.475 kb on + strand, within PGA1_c12350 at 1285.482 kb on + strand, within PGA1_c12350 at 1285.483 kb on - strand, within PGA1_c12350 at 1285.563 kb on + strand, within PGA1_c12350 at 1285.563 kb on + strand, within PGA1_c12350 at 1285.600 kb on + strand, within PGA1_c12350 at 1285.608 kb on - strand, within PGA1_c12350 at 1285.623 kb on + strand, within PGA1_c12350 at 1285.631 kb on - strand, within PGA1_c12350 at 1285.647 kb on + strand, within PGA1_c12350 at 1285.695 kb on - strand, within PGA1_c12350 at 1285.767 kb on - strand at 1285.856 kb on - strand at 1285.864 kb on - strand at 1285.916 kb on - strand at 1285.916 kb on - strand at 1285.926 kb on - strand at 1285.976 kb on - strand at 1285.984 kb on + strand at 1285.984 kb on + strand at 1286.001 kb on + strand at 1286.016 kb on - strand at 1286.029 kb on + strand at 1286.045 kb on + strand, within PGA1_c12360 at 1286.061 kb on + strand, within PGA1_c12360 at 1286.061 kb on + strand, within PGA1_c12360 at 1286.066 kb on + strand, within PGA1_c12360 at 1286.072 kb on + strand, within PGA1_c12360 at 1286.074 kb on - strand, within PGA1_c12360 at 1286.084 kb on - strand, within PGA1_c12360 at 1286.084 kb on - strand, within PGA1_c12360 at 1286.095 kb on + strand, within PGA1_c12360 at 1286.161 kb on + strand, within PGA1_c12360 at 1286.218 kb on - strand, within PGA1_c12360 at 1286.219 kb on + strand, within PGA1_c12360 at 1286.227 kb on - strand, within PGA1_c12360 at 1286.281 kb on + strand, within PGA1_c12360 at 1286.289 kb on - strand, within PGA1_c12360 at 1286.354 kb on + strand, within PGA1_c12360 at 1286.354 kb on + strand, within PGA1_c12360 at 1286.362 kb on - strand, within PGA1_c12360 at 1286.384 kb on - strand, within PGA1_c12360 at 1286.441 kb on - strand, within PGA1_c12360 at 1286.524 kb on - strand at 1286.525 kb on + strand at 1286.538 kb on + strand at 1286.554 kb on + strand at 1286.554 kb on + strand at 1286.610 kb on + strand at 1286.627 kb on + strand at 1286.668 kb on + strand at 1286.757 kb on + strand, within PGA1_c12370 at 1286.757 kb on - strand, within PGA1_c12370 at 1286.830 kb on + strand, within PGA1_c12370 at 1286.874 kb on + strand, within PGA1_c12370 at 1286.961 kb on - strand, within PGA1_c12370 at 1286.966 kb on + strand, within PGA1_c12370 at 1286.966 kb on + strand, within PGA1_c12370 at 1286.966 kb on + strand, within PGA1_c12370 at 1286.974 kb on - strand, within PGA1_c12370 at 1286.974 kb on - strand, within PGA1_c12370 at 1287.040 kb on - strand, within PGA1_c12370 at 1287.083 kb on + strand, within PGA1_c12370 at 1287.158 kb on + strand, within PGA1_c12370 at 1287.291 kb on + strand, within PGA1_c12370 at 1287.303 kb on + strand, within PGA1_c12370 at 1287.316 kb on - strand, within PGA1_c12370 at 1287.334 kb on + strand, within PGA1_c12370 at 1287.341 kb on + strand, within PGA1_c12370 at 1287.462 kb on - strand, within PGA1_c12370 at 1287.474 kb on + strand, within PGA1_c12370 at 1287.479 kb on + strand, within PGA1_c12370 at 1287.479 kb on + strand, within PGA1_c12370 at 1287.479 kb on + strand, within PGA1_c12370 at 1287.487 kb on - strand, within PGA1_c12370 at 1287.487 kb on - strand, within PGA1_c12370 at 1287.493 kb on + strand, within PGA1_c12370
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 1,285,044 + PGA1_c12350 0.48 +0.9 1,285,127 - PGA1_c12350 0.53 -0.3 1,285,171 - PGA1_c12350 0.56 +0.5 1,285,256 - PGA1_c12350 0.61 -0.3 1,285,306 + PGA1_c12350 0.64 -0.6 1,285,329 + PGA1_c12350 0.65 +1.0 1,285,334 + PGA1_c12350 0.66 -1.1 1,285,402 + PGA1_c12350 0.70 -0.7 1,285,438 + PGA1_c12350 0.72 -2.6 1,285,438 + PGA1_c12350 0.72 +0.3 1,285,446 - PGA1_c12350 0.72 -0.3 1,285,475 + PGA1_c12350 0.74 -1.2 1,285,482 + PGA1_c12350 0.75 +0.0 1,285,483 - PGA1_c12350 0.75 +0.2 1,285,563 + PGA1_c12350 0.79 +0.7 1,285,563 + PGA1_c12350 0.79 +0.4 1,285,600 + PGA1_c12350 0.82 +0.1 1,285,608 - PGA1_c12350 0.82 -0.5 1,285,623 + PGA1_c12350 0.83 -1.1 1,285,631 - PGA1_c12350 0.83 +0.1 1,285,647 + PGA1_c12350 0.84 +0.3 1,285,695 - PGA1_c12350 0.87 +0.9 1,285,767 - +1.1 1,285,856 - -1.1 1,285,864 - -0.0 1,285,916 - +0.9 1,285,916 - +0.3 1,285,926 - +0.8 1,285,976 - +1.5 1,285,984 + +2.1 1,285,984 + +0.3 1,286,001 + +0.8 1,286,016 - -2.2 1,286,029 + +1.5 1,286,045 + PGA1_c12360 0.11 -0.5 1,286,061 + PGA1_c12360 0.14 +0.1 1,286,061 + PGA1_c12360 0.14 -1.0 1,286,066 + PGA1_c12360 0.15 -1.9 1,286,072 + PGA1_c12360 0.16 +1.2 1,286,074 - PGA1_c12360 0.16 -1.0 1,286,084 - PGA1_c12360 0.18 +0.3 1,286,084 - PGA1_c12360 0.18 -1.2 1,286,095 + PGA1_c12360 0.20 +1.9 1,286,161 + PGA1_c12360 0.32 -2.0 1,286,218 - PGA1_c12360 0.43 -0.9 1,286,219 + PGA1_c12360 0.43 +0.3 1,286,227 - PGA1_c12360 0.44 -0.5 1,286,281 + PGA1_c12360 0.54 +0.7 1,286,289 - PGA1_c12360 0.56 -1.0 1,286,354 + PGA1_c12360 0.68 -0.2 1,286,354 + PGA1_c12360 0.68 +1.1 1,286,362 - PGA1_c12360 0.69 -2.0 1,286,384 - PGA1_c12360 0.74 -0.2 1,286,441 - PGA1_c12360 0.84 -0.8 1,286,524 - +0.5 1,286,525 + -1.5 1,286,538 + +3.5 1,286,554 + -1.7 1,286,554 + -1.2 1,286,610 + +0.2 1,286,627 + -0.5 1,286,668 + +0.1 1,286,757 + PGA1_c12370 0.12 +0.9 1,286,757 - PGA1_c12370 0.12 -2.0 1,286,830 + PGA1_c12370 0.19 +0.6 1,286,874 + PGA1_c12370 0.23 +0.8 1,286,961 - PGA1_c12370 0.31 +0.4 1,286,966 + PGA1_c12370 0.31 -0.2 1,286,966 + PGA1_c12370 0.31 -1.9 1,286,966 + PGA1_c12370 0.31 +1.3 1,286,974 - PGA1_c12370 0.32 -0.1 1,286,974 - PGA1_c12370 0.32 -3.0 1,287,040 - PGA1_c12370 0.38 -0.5 1,287,083 + PGA1_c12370 0.42 +1.6 1,287,158 + PGA1_c12370 0.49 -2.0 1,287,291 + PGA1_c12370 0.62 -0.6 1,287,303 + PGA1_c12370 0.63 +1.4 1,287,316 - PGA1_c12370 0.64 +1.1 1,287,334 + PGA1_c12370 0.66 +0.1 1,287,341 + PGA1_c12370 0.66 -1.7 1,287,462 - PGA1_c12370 0.78 -0.1 1,287,474 + PGA1_c12370 0.79 +0.4 1,287,479 + PGA1_c12370 0.79 +0.4 1,287,479 + PGA1_c12370 0.79 -0.7 1,287,479 + PGA1_c12370 0.79 -0.2 1,287,487 - PGA1_c12370 0.80 +0.4 1,287,487 - PGA1_c12370 0.80 -0.9 1,287,493 + PGA1_c12370 0.81 -0.0
Or see this region's nucleotide sequence