Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c12060 and PGA1_c12070 are separated by 66 nucleotides PGA1_c12070 and PGA1_c12080 are separated by 133 nucleotides
PGA1_c12060: PGA1_c12060 - acyl-CoA dehydrogenase, at 1,253,829 to 1,254,980
_c12060
PGA1_c12070: PGA1_c12070 - hypothetical protein, at 1,255,047 to 1,255,259
_c12070
PGA1_c12080: PGA1_c12080 - putative acyl-CoA dehydrogenase, at 1,255,393 to 1,257,174
_c12080
Position (kb)
1255
1256 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1254.173 kb on + strand, within PGA1_c12060 at 1254.188 kb on + strand, within PGA1_c12060 at 1254.275 kb on + strand, within PGA1_c12060 at 1254.276 kb on + strand, within PGA1_c12060 at 1254.286 kb on - strand, within PGA1_c12060 at 1254.332 kb on + strand, within PGA1_c12060 at 1254.332 kb on + strand, within PGA1_c12060 at 1254.332 kb on + strand, within PGA1_c12060 at 1254.332 kb on + strand, within PGA1_c12060 at 1254.340 kb on - strand, within PGA1_c12060 at 1254.340 kb on - strand, within PGA1_c12060 at 1254.340 kb on - strand, within PGA1_c12060 at 1254.382 kb on + strand, within PGA1_c12060 at 1254.382 kb on + strand, within PGA1_c12060 at 1254.390 kb on - strand, within PGA1_c12060 at 1254.390 kb on - strand, within PGA1_c12060 at 1254.390 kb on - strand, within PGA1_c12060 at 1254.424 kb on + strand, within PGA1_c12060 at 1254.426 kb on - strand, within PGA1_c12060 at 1254.430 kb on + strand, within PGA1_c12060 at 1254.431 kb on + strand, within PGA1_c12060 at 1254.439 kb on - strand, within PGA1_c12060 at 1254.448 kb on + strand, within PGA1_c12060 at 1254.522 kb on - strand, within PGA1_c12060 at 1254.529 kb on + strand, within PGA1_c12060 at 1254.529 kb on - strand, within PGA1_c12060 at 1254.664 kb on + strand, within PGA1_c12060 at 1254.664 kb on - strand, within PGA1_c12060 at 1254.672 kb on - strand, within PGA1_c12060 at 1254.786 kb on + strand, within PGA1_c12060 at 1254.794 kb on - strand, within PGA1_c12060 at 1254.808 kb on + strand, within PGA1_c12060 at 1254.808 kb on - strand, within PGA1_c12060 at 1254.813 kb on + strand, within PGA1_c12060 at 1254.813 kb on + strand, within PGA1_c12060 at 1254.813 kb on + strand, within PGA1_c12060 at 1254.816 kb on - strand, within PGA1_c12060 at 1254.926 kb on - strand at 1254.963 kb on + strand at 1254.971 kb on - strand at 1255.044 kb on + strand at 1255.052 kb on - strand at 1255.067 kb on - strand at 1255.090 kb on - strand, within PGA1_c12070 at 1255.157 kb on - strand, within PGA1_c12070 at 1255.160 kb on + strand, within PGA1_c12070 at 1255.164 kb on - strand, within PGA1_c12070 at 1255.225 kb on + strand, within PGA1_c12070 at 1255.255 kb on + strand at 1255.290 kb on - strand at 1255.399 kb on - strand at 1255.399 kb on - strand at 1255.409 kb on - strand at 1255.424 kb on - strand at 1255.473 kb on + strand at 1255.555 kb on - strand at 1255.582 kb on + strand, within PGA1_c12080 at 1255.582 kb on + strand, within PGA1_c12080 at 1255.582 kb on + strand, within PGA1_c12080 at 1255.582 kb on + strand, within PGA1_c12080 at 1255.585 kb on - strand, within PGA1_c12080 at 1255.590 kb on - strand, within PGA1_c12080 at 1255.590 kb on - strand, within PGA1_c12080 at 1255.675 kb on + strand, within PGA1_c12080 at 1255.680 kb on + strand, within PGA1_c12080 at 1255.690 kb on + strand, within PGA1_c12080 at 1255.690 kb on + strand, within PGA1_c12080 at 1255.698 kb on - strand, within PGA1_c12080 at 1255.726 kb on - strand, within PGA1_c12080 at 1255.792 kb on - strand, within PGA1_c12080 at 1255.943 kb on + strand, within PGA1_c12080 at 1255.943 kb on + strand, within PGA1_c12080 at 1255.951 kb on - strand, within PGA1_c12080 at 1255.951 kb on - strand, within PGA1_c12080 at 1255.971 kb on + strand, within PGA1_c12080 at 1256.019 kb on - strand, within PGA1_c12080 at 1256.023 kb on + strand, within PGA1_c12080 at 1256.088 kb on + strand, within PGA1_c12080 at 1256.088 kb on + strand, within PGA1_c12080 at 1256.088 kb on + strand, within PGA1_c12080 at 1256.088 kb on + strand, within PGA1_c12080 at 1256.096 kb on - strand, within PGA1_c12080 at 1256.096 kb on - strand, within PGA1_c12080 at 1256.211 kb on + strand, within PGA1_c12080 at 1256.219 kb on - strand, within PGA1_c12080 at 1256.250 kb on - strand, within PGA1_c12080 at 1256.255 kb on + strand, within PGA1_c12080 at 1256.255 kb on + strand, within PGA1_c12080
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 1,254,173 + PGA1_c12060 0.30 +0.4 1,254,188 + PGA1_c12060 0.31 -0.5 1,254,275 + PGA1_c12060 0.39 +2.1 1,254,276 + PGA1_c12060 0.39 -2.0 1,254,286 - PGA1_c12060 0.40 +0.3 1,254,332 + PGA1_c12060 0.44 -0.1 1,254,332 + PGA1_c12060 0.44 +2.5 1,254,332 + PGA1_c12060 0.44 -0.2 1,254,332 + PGA1_c12060 0.44 +0.5 1,254,340 - PGA1_c12060 0.44 +0.4 1,254,340 - PGA1_c12060 0.44 -1.8 1,254,340 - PGA1_c12060 0.44 +0.5 1,254,382 + PGA1_c12060 0.48 +0.1 1,254,382 + PGA1_c12060 0.48 -0.9 1,254,390 - PGA1_c12060 0.49 -0.7 1,254,390 - PGA1_c12060 0.49 -0.2 1,254,390 - PGA1_c12060 0.49 -0.2 1,254,424 + PGA1_c12060 0.52 -0.7 1,254,426 - PGA1_c12060 0.52 -0.9 1,254,430 + PGA1_c12060 0.52 -0.6 1,254,431 + PGA1_c12060 0.52 +0.6 1,254,439 - PGA1_c12060 0.53 -0.2 1,254,448 + PGA1_c12060 0.54 -0.7 1,254,522 - PGA1_c12060 0.60 -1.2 1,254,529 + PGA1_c12060 0.61 -0.1 1,254,529 - PGA1_c12060 0.61 +1.1 1,254,664 + PGA1_c12060 0.72 -1.2 1,254,664 - PGA1_c12060 0.72 +1.6 1,254,672 - PGA1_c12060 0.73 -0.6 1,254,786 + PGA1_c12060 0.83 +1.1 1,254,794 - PGA1_c12060 0.84 +0.3 1,254,808 + PGA1_c12060 0.85 -0.9 1,254,808 - PGA1_c12060 0.85 -1.0 1,254,813 + PGA1_c12060 0.85 -1.1 1,254,813 + PGA1_c12060 0.85 -2.4 1,254,813 + PGA1_c12060 0.85 +0.1 1,254,816 - PGA1_c12060 0.86 -0.3 1,254,926 - +0.0 1,254,963 + +2.1 1,254,971 - +0.1 1,255,044 + +0.9 1,255,052 - -0.3 1,255,067 - +1.7 1,255,090 - PGA1_c12070 0.20 +0.1 1,255,157 - PGA1_c12070 0.52 +1.9 1,255,160 + PGA1_c12070 0.53 +0.3 1,255,164 - PGA1_c12070 0.55 +0.2 1,255,225 + PGA1_c12070 0.84 +1.4 1,255,255 + -0.3 1,255,290 - +1.1 1,255,399 - +0.7 1,255,399 - -0.4 1,255,409 - -0.0 1,255,424 - -0.9 1,255,473 + +0.7 1,255,555 - -1.6 1,255,582 + PGA1_c12080 0.11 -0.9 1,255,582 + PGA1_c12080 0.11 -1.4 1,255,582 + PGA1_c12080 0.11 -0.3 1,255,582 + PGA1_c12080 0.11 +1.1 1,255,585 - PGA1_c12080 0.11 -0.8 1,255,590 - PGA1_c12080 0.11 -0.9 1,255,590 - PGA1_c12080 0.11 -1.0 1,255,675 + PGA1_c12080 0.16 -1.2 1,255,680 + PGA1_c12080 0.16 +0.3 1,255,690 + PGA1_c12080 0.17 +0.8 1,255,690 + PGA1_c12080 0.17 +0.1 1,255,698 - PGA1_c12080 0.17 -0.9 1,255,726 - PGA1_c12080 0.19 +0.8 1,255,792 - PGA1_c12080 0.22 -0.5 1,255,943 + PGA1_c12080 0.31 -1.0 1,255,943 + PGA1_c12080 0.31 +0.1 1,255,951 - PGA1_c12080 0.31 +0.1 1,255,951 - PGA1_c12080 0.31 -1.5 1,255,971 + PGA1_c12080 0.32 +2.4 1,256,019 - PGA1_c12080 0.35 -0.2 1,256,023 + PGA1_c12080 0.35 -1.1 1,256,088 + PGA1_c12080 0.39 -0.6 1,256,088 + PGA1_c12080 0.39 -0.7 1,256,088 + PGA1_c12080 0.39 -1.3 1,256,088 + PGA1_c12080 0.39 +0.3 1,256,096 - PGA1_c12080 0.39 +0.9 1,256,096 - PGA1_c12080 0.39 -2.0 1,256,211 + PGA1_c12080 0.46 +0.1 1,256,219 - PGA1_c12080 0.46 -0.5 1,256,250 - PGA1_c12080 0.48 -1.2 1,256,255 + PGA1_c12080 0.48 +0.9 1,256,255 + PGA1_c12080 0.48 -1.0
Or see this region's nucleotide sequence