Experiment: DinoMM minimal media with L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11520 and PGA1_c11530 overlap by 1 nucleotides PGA1_c11530 and PGA1_c11540 are separated by 93 nucleotides
PGA1_c11520: PGA1_c11520 - gamma-glutamylputrescine synthetase PuuA, at 1,191,266 to 1,192,624
_c11520
PGA1_c11530: PGA1_c11530 - putative glutamine amidotransferase class I, at 1,192,624 to 1,193,304
_c11530
PGA1_c11540: PGA1_c11540 - gamma-glutamylputrescine synthetase PuuA, at 1,193,398 to 1,194,744
_c11540
Position (kb)
1192
1193
1194 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1191.877 kb on - strand, within PGA1_c11520 at 1191.928 kb on + strand, within PGA1_c11520 at 1191.928 kb on - strand, within PGA1_c11520 at 1192.012 kb on + strand, within PGA1_c11520 at 1192.014 kb on - strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.034 kb on + strand, within PGA1_c11520 at 1192.042 kb on - strand, within PGA1_c11520 at 1192.101 kb on + strand, within PGA1_c11520 at 1192.167 kb on + strand, within PGA1_c11520 at 1192.168 kb on + strand, within PGA1_c11520 at 1192.234 kb on - strand, within PGA1_c11520 at 1192.241 kb on + strand, within PGA1_c11520 at 1192.244 kb on - strand, within PGA1_c11520 at 1192.259 kb on - strand, within PGA1_c11520 at 1192.281 kb on - strand, within PGA1_c11520 at 1192.349 kb on - strand, within PGA1_c11520 at 1192.365 kb on + strand, within PGA1_c11520 at 1192.509 kb on + strand at 1192.517 kb on - strand at 1192.524 kb on + strand at 1192.583 kb on + strand at 1192.591 kb on - strand at 1192.591 kb on - strand at 1192.593 kb on + strand at 1192.620 kb on + strand at 1192.693 kb on - strand, within PGA1_c11530 at 1192.736 kb on - strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on + strand, within PGA1_c11530 at 1192.802 kb on - strand, within PGA1_c11530 at 1192.810 kb on - strand, within PGA1_c11530 at 1192.810 kb on - strand, within PGA1_c11530 at 1192.810 kb on - strand, within PGA1_c11530 at 1192.810 kb on - strand, within PGA1_c11530 at 1192.879 kb on - strand, within PGA1_c11530 at 1192.879 kb on - strand, within PGA1_c11530 at 1192.883 kb on + strand, within PGA1_c11530 at 1192.958 kb on + strand, within PGA1_c11530 at 1193.001 kb on + strand, within PGA1_c11530 at 1193.016 kb on - strand, within PGA1_c11530 at 1193.126 kb on + strand, within PGA1_c11530 at 1193.189 kb on - strand, within PGA1_c11530 at 1193.189 kb on - strand, within PGA1_c11530 at 1193.263 kb on + strand at 1193.776 kb on + strand, within PGA1_c11540
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Threonine remove 1,191,877 - PGA1_c11520 0.45 +0.5 1,191,928 + PGA1_c11520 0.49 -0.1 1,191,928 - PGA1_c11520 0.49 +0.9 1,192,012 + PGA1_c11520 0.55 -1.2 1,192,014 - PGA1_c11520 0.55 -1.7 1,192,034 + PGA1_c11520 0.57 -0.1 1,192,034 + PGA1_c11520 0.57 +1.9 1,192,034 + PGA1_c11520 0.57 +0.3 1,192,034 + PGA1_c11520 0.57 +0.3 1,192,034 + PGA1_c11520 0.57 +0.9 1,192,042 - PGA1_c11520 0.57 -0.3 1,192,101 + PGA1_c11520 0.61 +1.3 1,192,167 + PGA1_c11520 0.66 +0.3 1,192,168 + PGA1_c11520 0.66 +0.6 1,192,234 - PGA1_c11520 0.71 +0.5 1,192,241 + PGA1_c11520 0.72 +0.0 1,192,244 - PGA1_c11520 0.72 +0.2 1,192,259 - PGA1_c11520 0.73 +0.1 1,192,281 - PGA1_c11520 0.75 -0.1 1,192,349 - PGA1_c11520 0.80 -0.6 1,192,365 + PGA1_c11520 0.81 -2.5 1,192,509 + +0.6 1,192,517 - -0.5 1,192,524 + +1.9 1,192,583 + +1.3 1,192,591 - -1.9 1,192,591 - -0.5 1,192,593 + -0.5 1,192,620 + +0.8 1,192,693 - PGA1_c11530 0.10 +0.3 1,192,736 - PGA1_c11530 0.16 -1.3 1,192,802 + PGA1_c11530 0.26 -1.2 1,192,802 + PGA1_c11530 0.26 -0.2 1,192,802 + PGA1_c11530 0.26 +0.1 1,192,802 + PGA1_c11530 0.26 -0.0 1,192,802 + PGA1_c11530 0.26 -3.0 1,192,802 + PGA1_c11530 0.26 -0.3 1,192,802 + PGA1_c11530 0.26 +0.1 1,192,802 - PGA1_c11530 0.26 -0.3 1,192,810 - PGA1_c11530 0.27 -0.4 1,192,810 - PGA1_c11530 0.27 +1.1 1,192,810 - PGA1_c11530 0.27 -1.0 1,192,810 - PGA1_c11530 0.27 -0.0 1,192,879 - PGA1_c11530 0.37 +0.2 1,192,879 - PGA1_c11530 0.37 -0.5 1,192,883 + PGA1_c11530 0.38 -0.6 1,192,958 + PGA1_c11530 0.49 -0.3 1,193,001 + PGA1_c11530 0.55 +1.1 1,193,016 - PGA1_c11530 0.58 -1.0 1,193,126 + PGA1_c11530 0.74 -0.3 1,193,189 - PGA1_c11530 0.83 -1.3 1,193,189 - PGA1_c11530 0.83 -0.6 1,193,263 + +0.1 1,193,776 + PGA1_c11540 0.28 -0.2
Or see this region's nucleotide sequence