Experiment: Varel_Bryant_medium_Glucose with Metronidazole 0.0015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4139 and BT4140 are separated by 87 nucleotides BT4140 and BT4141 are separated by 466 nucleotides
BT4139: BT4139 - conserved hypothetical protein, with a calcineurin-like phosphoesterase domain (NCBI ptt file), at 5,434,682 to 5,435,773
BT4139
BT4140: BT4140 - hypothetical protein (NCBI ptt file), at 5,435,861 to 5,436,175
BT4140
BT4141: BT4141 - hypothetical protein (NCBI ptt file), at 5,436,642 to 5,436,974
BT4141
Position (kb)
5435
5436
5437 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5434.911 kb on + strand, within BT4139 at 5434.923 kb on - strand, within BT4139 at 5434.944 kb on + strand, within BT4139 at 5434.945 kb on - strand, within BT4139 at 5434.962 kb on + strand, within BT4139 at 5434.962 kb on + strand, within BT4139 at 5434.991 kb on + strand, within BT4139 at 5434.991 kb on + strand, within BT4139 at 5434.992 kb on - strand, within BT4139 at 5435.027 kb on + strand, within BT4139 at 5435.037 kb on - strand at 5435.038 kb on + strand, within BT4139 at 5435.039 kb on - strand, within BT4139 at 5435.042 kb on + strand, within BT4139 at 5435.043 kb on - strand, within BT4139 at 5435.092 kb on - strand, within BT4139 at 5435.136 kb on + strand, within BT4139 at 5435.137 kb on - strand, within BT4139 at 5435.176 kb on - strand, within BT4139 at 5435.176 kb on - strand, within BT4139 at 5435.180 kb on - strand, within BT4139 at 5435.233 kb on + strand, within BT4139 at 5435.233 kb on + strand, within BT4139 at 5435.233 kb on + strand, within BT4139 at 5435.250 kb on + strand, within BT4139 at 5435.252 kb on + strand, within BT4139 at 5435.252 kb on + strand, within BT4139 at 5435.252 kb on + strand, within BT4139 at 5435.253 kb on - strand, within BT4139 at 5435.253 kb on - strand, within BT4139 at 5435.308 kb on + strand, within BT4139 at 5435.330 kb on + strand, within BT4139 at 5435.372 kb on - strand, within BT4139 at 5435.380 kb on - strand at 5435.403 kb on + strand, within BT4139 at 5435.474 kb on - strand, within BT4139 at 5435.478 kb on + strand, within BT4139 at 5435.479 kb on - strand, within BT4139 at 5435.479 kb on - strand, within BT4139 at 5435.498 kb on - strand, within BT4139 at 5435.532 kb on - strand, within BT4139 at 5435.532 kb on - strand, within BT4139 at 5435.585 kb on - strand, within BT4139 at 5435.601 kb on + strand, within BT4139 at 5435.608 kb on + strand, within BT4139 at 5435.609 kb on - strand, within BT4139 at 5435.631 kb on + strand, within BT4139 at 5435.631 kb on + strand, within BT4139 at 5435.632 kb on - strand, within BT4139 at 5435.633 kb on + strand, within BT4139 at 5435.674 kb on + strand at 5435.679 kb on - strand at 5435.679 kb on - strand at 5435.694 kb on + strand at 5435.782 kb on + strand at 5435.840 kb on + strand at 5435.841 kb on - strand at 5435.861 kb on - strand at 5435.932 kb on - strand, within BT4140 at 5435.932 kb on - strand, within BT4140 at 5435.932 kb on - strand, within BT4140 at 5435.934 kb on + strand, within BT4140 at 5435.934 kb on + strand, within BT4140 at 5435.934 kb on + strand, within BT4140 at 5435.935 kb on - strand, within BT4140 at 5435.936 kb on + strand, within BT4140 at 5435.936 kb on + strand, within BT4140 at 5435.977 kb on + strand, within BT4140 at 5436.037 kb on + strand, within BT4140 at 5436.079 kb on + strand, within BT4140 at 5436.080 kb on - strand, within BT4140 at 5436.095 kb on - strand, within BT4140 at 5436.140 kb on + strand, within BT4140 at 5436.141 kb on - strand, within BT4140 at 5436.141 kb on - strand, within BT4140 at 5436.169 kb on - strand at 5436.172 kb on - strand at 5436.198 kb on - strand at 5436.256 kb on + strand at 5436.258 kb on + strand at 5436.348 kb on + strand at 5436.371 kb on - strand at 5436.373 kb on - strand at 5436.373 kb on - strand at 5436.447 kb on - strand at 5436.461 kb on + strand at 5436.478 kb on - strand at 5436.505 kb on + strand at 5436.505 kb on + strand at 5436.509 kb on + strand at 5436.509 kb on + strand at 5436.510 kb on - strand at 5436.510 kb on - strand at 5436.518 kb on + strand at 5436.520 kb on + strand at 5436.521 kb on - strand at 5436.522 kb on + strand at 5436.544 kb on + strand at 5436.544 kb on + strand at 5436.545 kb on - strand at 5436.549 kb on - strand at 5436.599 kb on - strand at 5436.707 kb on - strand, within BT4141 at 5436.707 kb on - strand, within BT4141 at 5436.801 kb on - strand, within BT4141 at 5436.845 kb on - strand, within BT4141 at 5436.855 kb on + strand, within BT4141 at 5436.856 kb on - strand, within BT4141 at 5436.876 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.902 kb on - strand, within BT4141 at 5436.904 kb on - strand, within BT4141 at 5436.955 kb on + strand at 5436.955 kb on + strand at 5437.077 kb on - strand at 5437.143 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Metronidazole 0.0015625 mM remove 5,434,911 + BT4139 0.21 -2.8 5,434,923 - BT4139 0.22 -1.0 5,434,944 + BT4139 0.24 -1.7 5,434,945 - BT4139 0.24 -1.4 5,434,962 + BT4139 0.26 -1.4 5,434,962 + BT4139 0.26 +1.0 5,434,991 + BT4139 0.28 +2.3 5,434,991 + BT4139 0.28 -0.4 5,434,992 - BT4139 0.28 -0.0 5,435,027 + BT4139 0.32 -0.0 5,435,037 - +0.6 5,435,038 + BT4139 0.33 -0.0 5,435,039 - BT4139 0.33 +0.6 5,435,042 + BT4139 0.33 -0.0 5,435,043 - BT4139 0.33 +0.4 5,435,092 - BT4139 0.38 -0.8 5,435,136 + BT4139 0.42 -2.0 5,435,137 - BT4139 0.42 -1.8 5,435,176 - BT4139 0.45 -2.6 5,435,176 - BT4139 0.45 -0.0 5,435,180 - BT4139 0.46 -3.2 5,435,233 + BT4139 0.50 -0.8 5,435,233 + BT4139 0.50 -0.0 5,435,233 + BT4139 0.50 -2.0 5,435,250 + BT4139 0.52 -0.3 5,435,252 + BT4139 0.52 -0.0 5,435,252 + BT4139 0.52 -0.9 5,435,252 + BT4139 0.52 -1.6 5,435,253 - BT4139 0.52 -3.9 5,435,253 - BT4139 0.52 -0.7 5,435,308 + BT4139 0.57 -2.0 5,435,330 + BT4139 0.59 -2.0 5,435,372 - BT4139 0.63 -0.0 5,435,380 - -1.0 5,435,403 + BT4139 0.66 -0.4 5,435,474 - BT4139 0.73 -2.0 5,435,478 + BT4139 0.73 -0.3 5,435,479 - BT4139 0.73 -0.9 5,435,479 - BT4139 0.73 -1.0 5,435,498 - BT4139 0.75 -0.0 5,435,532 - BT4139 0.78 -1.0 5,435,532 - BT4139 0.78 -2.8 5,435,585 - BT4139 0.83 -3.0 5,435,601 + BT4139 0.84 +0.5 5,435,608 + BT4139 0.85 -1.0 5,435,609 - BT4139 0.85 -2.0 5,435,631 + BT4139 0.87 -1.0 5,435,631 + BT4139 0.87 -1.0 5,435,632 - BT4139 0.87 -1.2 5,435,633 + BT4139 0.87 -0.6 5,435,674 + +2.0 5,435,679 - -0.8 5,435,679 - -1.2 5,435,694 + -0.5 5,435,782 + -0.0 5,435,840 + -0.0 5,435,841 - +0.8 5,435,861 - -0.9 5,435,932 - BT4140 0.23 -1.1 5,435,932 - BT4140 0.23 -1.5 5,435,932 - BT4140 0.23 -1.8 5,435,934 + BT4140 0.23 -2.0 5,435,934 + BT4140 0.23 -1.6 5,435,934 + BT4140 0.23 -1.2 5,435,935 - BT4140 0.23 -0.0 5,435,936 + BT4140 0.24 -2.4 5,435,936 + BT4140 0.24 -1.4 5,435,977 + BT4140 0.37 -2.0 5,436,037 + BT4140 0.56 -1.3 5,436,079 + BT4140 0.69 -2.6 5,436,080 - BT4140 0.70 -0.9 5,436,095 - BT4140 0.74 -1.4 5,436,140 + BT4140 0.89 -0.0 5,436,141 - BT4140 0.89 +0.2 5,436,141 - BT4140 0.89 -1.3 5,436,169 - -1.8 5,436,172 - -0.2 5,436,198 - -1.0 5,436,256 + -0.0 5,436,258 + -1.4 5,436,348 + -0.3 5,436,371 - -2.0 5,436,373 - -0.0 5,436,373 - -0.0 5,436,447 - -0.0 5,436,461 + -1.0 5,436,478 - -0.0 5,436,505 + -1.6 5,436,505 + -1.1 5,436,509 + -1.1 5,436,509 + -0.8 5,436,510 - -0.7 5,436,510 - -0.2 5,436,518 + -0.8 5,436,520 + -2.0 5,436,521 - +1.3 5,436,522 + +0.1 5,436,544 + -1.5 5,436,544 + -0.4 5,436,545 - -0.0 5,436,549 - -1.8 5,436,599 - -0.0 5,436,707 - BT4141 0.20 -0.1 5,436,707 - BT4141 0.20 -2.2 5,436,801 - BT4141 0.48 -2.1 5,436,845 - BT4141 0.61 -1.0 5,436,855 + BT4141 0.64 +1.0 5,436,856 - BT4141 0.64 -1.0 5,436,876 - BT4141 0.70 -3.2 5,436,902 - BT4141 0.78 -0.4 5,436,902 - BT4141 0.78 -2.4 5,436,902 - BT4141 0.78 -1.6 5,436,902 - BT4141 0.78 +0.5 5,436,904 - BT4141 0.79 -0.0 5,436,955 + -0.9 5,436,955 + -0.0 5,437,077 - -0.6 5,437,143 + -1.6
Or see this region's nucleotide sequence