Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c34390 and PGA1_c34400 are separated by 354 nucleotides PGA1_c34400 and PGA1_c34410 are separated by 65 nucleotides PGA1_c34410 and PGA1_c34420 are separated by 105 nucleotides
PGA1_c34390: PGA1_c34390 - spermidine/putrescine import ATP-binding protein PotA, at 3,605,666 to 3,606,766
_c34390
PGA1_c34400: PGA1_c34400 - aminotransferase class-III, at 3,607,121 to 3,608,515
_c34400
PGA1_c34410: PGA1_c34410 - HTH-type transcriptional regulator, GntR family, at 3,608,581 to 3,609,303
_c34410
PGA1_c34420: PGA1_c34420 - putrescine-binding periplasmic protein PotF, at 3,609,409 to 3,610,494
_c34420
Position (kb)
3607
3608
3609 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3606.149 kb on - strand, within PGA1_c34390 at 3606.159 kb on + strand, within PGA1_c34390 at 3606.202 kb on - strand, within PGA1_c34390 at 3606.351 kb on + strand, within PGA1_c34390 at 3606.352 kb on + strand, within PGA1_c34390 at 3606.404 kb on - strand, within PGA1_c34390 at 3606.420 kb on + strand, within PGA1_c34390 at 3606.428 kb on - strand, within PGA1_c34390 at 3606.450 kb on + strand, within PGA1_c34390 at 3606.460 kb on - strand, within PGA1_c34390 at 3606.485 kb on - strand, within PGA1_c34390 at 3606.516 kb on - strand, within PGA1_c34390 at 3606.532 kb on + strand, within PGA1_c34390 at 3606.570 kb on - strand, within PGA1_c34390 at 3606.582 kb on + strand, within PGA1_c34390 at 3606.615 kb on + strand, within PGA1_c34390 at 3606.615 kb on + strand, within PGA1_c34390 at 3606.682 kb on - strand at 3606.682 kb on - strand at 3606.702 kb on - strand at 3606.749 kb on + strand at 3606.755 kb on - strand at 3606.775 kb on + strand at 3606.826 kb on + strand at 3606.826 kb on + strand at 3606.826 kb on + strand at 3606.828 kb on + strand at 3606.835 kb on - strand at 3606.836 kb on - strand at 3606.862 kb on - strand at 3606.903 kb on - strand at 3606.987 kb on - strand at 3607.002 kb on - strand at 3607.014 kb on + strand at 3607.073 kb on + strand at 3607.112 kb on + strand at 3607.120 kb on + strand at 3607.147 kb on - strand at 3607.371 kb on - strand, within PGA1_c34400 at 3607.389 kb on + strand, within PGA1_c34400 at 3607.537 kb on + strand, within PGA1_c34400 at 3607.556 kb on - strand, within PGA1_c34400 at 3607.582 kb on - strand, within PGA1_c34400 at 3607.649 kb on - strand, within PGA1_c34400 at 3607.708 kb on + strand, within PGA1_c34400 at 3607.716 kb on - strand, within PGA1_c34400 at 3607.779 kb on + strand, within PGA1_c34400 at 3607.820 kb on + strand, within PGA1_c34400 at 3607.853 kb on + strand, within PGA1_c34400 at 3607.864 kb on + strand, within PGA1_c34400 at 3608.020 kb on + strand, within PGA1_c34400 at 3608.116 kb on + strand, within PGA1_c34400 at 3608.171 kb on + strand, within PGA1_c34400 at 3608.224 kb on + strand, within PGA1_c34400 at 3608.224 kb on + strand, within PGA1_c34400 at 3608.224 kb on + strand, within PGA1_c34400 at 3608.224 kb on + strand, within PGA1_c34400 at 3608.224 kb on + strand, within PGA1_c34400 at 3608.224 kb on + strand, within PGA1_c34400 at 3608.224 kb on + strand, within PGA1_c34400 at 3608.232 kb on - strand, within PGA1_c34400 at 3608.278 kb on - strand, within PGA1_c34400 at 3608.333 kb on - strand, within PGA1_c34400 at 3608.385 kb on - strand at 3608.385 kb on - strand at 3608.385 kb on - strand at 3608.468 kb on - strand at 3608.615 kb on + strand at 3608.615 kb on + strand at 3608.615 kb on + strand at 3608.661 kb on - strand, within PGA1_c34410 at 3608.667 kb on - strand, within PGA1_c34410 at 3608.679 kb on - strand, within PGA1_c34410 at 3608.682 kb on - strand, within PGA1_c34410 at 3608.682 kb on - strand, within PGA1_c34410 at 3608.713 kb on + strand, within PGA1_c34410 at 3608.713 kb on + strand, within PGA1_c34410 at 3608.716 kb on - strand, within PGA1_c34410 at 3608.720 kb on + strand, within PGA1_c34410 at 3608.748 kb on + strand, within PGA1_c34410 at 3608.754 kb on + strand, within PGA1_c34410 at 3608.754 kb on + strand, within PGA1_c34410 at 3608.789 kb on + strand, within PGA1_c34410 at 3608.813 kb on + strand, within PGA1_c34410 at 3608.813 kb on + strand, within PGA1_c34410 at 3608.852 kb on - strand, within PGA1_c34410 at 3608.902 kb on - strand, within PGA1_c34410 at 3608.927 kb on + strand, within PGA1_c34410 at 3609.005 kb on + strand, within PGA1_c34410 at 3609.013 kb on - strand, within PGA1_c34410 at 3609.024 kb on - strand, within PGA1_c34410 at 3609.024 kb on - strand, within PGA1_c34410 at 3609.026 kb on + strand, within PGA1_c34410 at 3609.137 kb on + strand, within PGA1_c34410 at 3609.237 kb on + strand at 3609.309 kb on - strand at 3609.316 kb on + strand at 3609.345 kb on + strand at 3609.398 kb on + strand at 3609.413 kb on - strand at 3609.413 kb on - strand at 3609.453 kb on - strand at 3609.508 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 3,606,149 - PGA1_c34390 0.44 +0.9 3,606,159 + PGA1_c34390 0.45 +0.2 3,606,202 - PGA1_c34390 0.49 -1.9 3,606,351 + PGA1_c34390 0.62 -1.7 3,606,352 + PGA1_c34390 0.62 +1.1 3,606,404 - PGA1_c34390 0.67 -1.4 3,606,420 + PGA1_c34390 0.68 -1.2 3,606,428 - PGA1_c34390 0.69 -1.9 3,606,450 + PGA1_c34390 0.71 +0.1 3,606,460 - PGA1_c34390 0.72 -1.9 3,606,485 - PGA1_c34390 0.74 -2.0 3,606,516 - PGA1_c34390 0.77 -0.9 3,606,532 + PGA1_c34390 0.79 +0.0 3,606,570 - PGA1_c34390 0.82 -1.1 3,606,582 + PGA1_c34390 0.83 -0.0 3,606,615 + PGA1_c34390 0.86 +0.0 3,606,615 + PGA1_c34390 0.86 -0.1 3,606,682 - -0.9 3,606,682 - +0.5 3,606,702 - +0.5 3,606,749 + +0.3 3,606,755 - -0.9 3,606,775 + +0.6 3,606,826 + -0.0 3,606,826 + -0.7 3,606,826 + -1.7 3,606,828 + -0.3 3,606,835 - +1.2 3,606,836 - -0.5 3,606,862 - +0.9 3,606,903 - +0.1 3,606,987 - +0.7 3,607,002 - -2.5 3,607,014 + -1.1 3,607,073 + -2.4 3,607,112 + +0.7 3,607,120 + +0.7 3,607,147 - -0.4 3,607,371 - PGA1_c34400 0.18 -1.7 3,607,389 + PGA1_c34400 0.19 -1.1 3,607,537 + PGA1_c34400 0.30 -2.9 3,607,556 - PGA1_c34400 0.31 +0.3 3,607,582 - PGA1_c34400 0.33 -0.6 3,607,649 - PGA1_c34400 0.38 -0.9 3,607,708 + PGA1_c34400 0.42 -1.0 3,607,716 - PGA1_c34400 0.43 -3.0 3,607,779 + PGA1_c34400 0.47 +1.7 3,607,820 + PGA1_c34400 0.50 -0.7 3,607,853 + PGA1_c34400 0.52 -1.1 3,607,864 + PGA1_c34400 0.53 -0.3 3,608,020 + PGA1_c34400 0.64 -0.1 3,608,116 + PGA1_c34400 0.71 -0.4 3,608,171 + PGA1_c34400 0.75 +0.5 3,608,224 + PGA1_c34400 0.79 -1.9 3,608,224 + PGA1_c34400 0.79 +0.5 3,608,224 + PGA1_c34400 0.79 +0.5 3,608,224 + PGA1_c34400 0.79 -0.1 3,608,224 + PGA1_c34400 0.79 -1.4 3,608,224 + PGA1_c34400 0.79 -1.7 3,608,224 + PGA1_c34400 0.79 +0.5 3,608,232 - PGA1_c34400 0.80 +0.5 3,608,278 - PGA1_c34400 0.83 -1.9 3,608,333 - PGA1_c34400 0.87 -0.5 3,608,385 - -0.6 3,608,385 - +1.4 3,608,385 - -1.7 3,608,468 - +0.0 3,608,615 + -1.4 3,608,615 + +0.4 3,608,615 + -1.8 3,608,661 - PGA1_c34410 0.11 -0.6 3,608,667 - PGA1_c34410 0.12 -0.1 3,608,679 - PGA1_c34410 0.14 -2.1 3,608,682 - PGA1_c34410 0.14 -1.6 3,608,682 - PGA1_c34410 0.14 +0.2 3,608,713 + PGA1_c34410 0.18 -0.7 3,608,713 + PGA1_c34410 0.18 -1.1 3,608,716 - PGA1_c34410 0.19 -1.1 3,608,720 + PGA1_c34410 0.19 -1.3 3,608,748 + PGA1_c34410 0.23 +0.9 3,608,754 + PGA1_c34410 0.24 -0.4 3,608,754 + PGA1_c34410 0.24 +0.3 3,608,789 + PGA1_c34410 0.29 -1.7 3,608,813 + PGA1_c34410 0.32 +2.4 3,608,813 + PGA1_c34410 0.32 -1.4 3,608,852 - PGA1_c34410 0.37 -0.9 3,608,902 - PGA1_c34410 0.44 -2.3 3,608,927 + PGA1_c34410 0.48 -0.4 3,609,005 + PGA1_c34410 0.59 +1.6 3,609,013 - PGA1_c34410 0.60 -1.4 3,609,024 - PGA1_c34410 0.61 -0.9 3,609,024 - PGA1_c34410 0.61 +1.0 3,609,026 + PGA1_c34410 0.62 +0.1 3,609,137 + PGA1_c34410 0.77 -1.8 3,609,237 + -0.1 3,609,309 - +1.7 3,609,316 + +0.4 3,609,345 + -0.4 3,609,398 + +0.0 3,609,413 - -0.3 3,609,413 - +0.6 3,609,453 - -0.1 3,609,508 + -0.7
Or see this region's nucleotide sequence