Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c07020 and PGA1_c07030 are separated by 89 nucleotides PGA1_c07030 and PGA1_c07040 are separated by 71 nucleotides
PGA1_c07020: PGA1_c07020 - Poly(3-hydroxybutyrate) depolymerase, at 720,752 to 721,573
_c07020
PGA1_c07030: PGA1_c07030 - phenylalanyl-tRNA synthetase beta chain, at 721,663 to 724,059
_c07030
PGA1_c07040: PGA1_c07040 - transcriptional regulator, lysR family, at 724,131 to 725,009
_c07040
Position (kb)
721
722
723
724
725 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 720.758 kb on + strand at 720.772 kb on + strand at 720.780 kb on - strand at 720.787 kb on + strand at 720.790 kb on - strand at 720.805 kb on - strand at 720.826 kb on + strand at 720.826 kb on + strand at 720.866 kb on - strand, within PGA1_c07020 at 720.879 kb on + strand, within PGA1_c07020 at 720.912 kb on + strand, within PGA1_c07020 at 720.912 kb on + strand, within PGA1_c07020 at 720.914 kb on - strand, within PGA1_c07020 at 720.914 kb on - strand, within PGA1_c07020 at 720.920 kb on - strand, within PGA1_c07020 at 720.988 kb on + strand, within PGA1_c07020 at 721.037 kb on - strand, within PGA1_c07020 at 721.079 kb on + strand, within PGA1_c07020 at 721.100 kb on + strand, within PGA1_c07020 at 721.100 kb on + strand, within PGA1_c07020 at 721.100 kb on + strand, within PGA1_c07020 at 721.100 kb on + strand, within PGA1_c07020 at 721.100 kb on + strand, within PGA1_c07020 at 721.143 kb on + strand, within PGA1_c07020 at 721.151 kb on - strand, within PGA1_c07020 at 721.211 kb on + strand, within PGA1_c07020 at 721.253 kb on + strand, within PGA1_c07020 at 721.369 kb on - strand, within PGA1_c07020 at 721.459 kb on - strand, within PGA1_c07020 at 721.459 kb on - strand, within PGA1_c07020 at 721.479 kb on + strand, within PGA1_c07020 at 721.499 kb on + strand at 721.574 kb on + strand at 721.591 kb on + strand at 724.087 kb on - strand at 724.160 kb on + strand at 724.160 kb on + strand at 724.160 kb on + strand at 724.168 kb on - strand at 724.168 kb on - strand at 724.168 kb on - strand at 724.168 kb on - strand at 724.235 kb on + strand, within PGA1_c07040 at 724.243 kb on - strand, within PGA1_c07040 at 724.243 kb on - strand, within PGA1_c07040 at 724.244 kb on - strand, within PGA1_c07040 at 724.246 kb on + strand, within PGA1_c07040 at 724.254 kb on - strand, within PGA1_c07040 at 724.277 kb on - strand, within PGA1_c07040 at 724.333 kb on - strand, within PGA1_c07040 at 724.383 kb on - strand, within PGA1_c07040 at 724.416 kb on + strand, within PGA1_c07040 at 724.416 kb on + strand, within PGA1_c07040 at 724.416 kb on + strand, within PGA1_c07040 at 724.416 kb on + strand, within PGA1_c07040 at 724.424 kb on - strand, within PGA1_c07040 at 724.424 kb on - strand, within PGA1_c07040 at 724.424 kb on - strand, within PGA1_c07040 at 724.424 kb on - strand, within PGA1_c07040 at 724.455 kb on + strand, within PGA1_c07040 at 724.494 kb on + strand, within PGA1_c07040 at 724.494 kb on + strand, within PGA1_c07040 at 724.494 kb on + strand, within PGA1_c07040 at 724.494 kb on + strand, within PGA1_c07040 at 724.504 kb on + strand, within PGA1_c07040 at 724.530 kb on + strand, within PGA1_c07040 at 724.564 kb on - strand, within PGA1_c07040 at 724.594 kb on + strand, within PGA1_c07040 at 724.656 kb on + strand, within PGA1_c07040 at 724.675 kb on + strand, within PGA1_c07040 at 724.910 kb on - strand, within PGA1_c07040 at 724.912 kb on + strand, within PGA1_c07040 at 724.915 kb on - strand, within PGA1_c07040 at 724.918 kb on + strand, within PGA1_c07040 at 724.922 kb on + strand at 724.922 kb on + strand at 724.922 kb on + strand at 724.930 kb on - strand at 724.930 kb on - strand at 724.937 kb on + strand at 724.937 kb on + strand at 724.941 kb on + strand at 724.947 kb on + strand at 724.978 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 720,758 + -1.5 720,772 + -1.3 720,780 - -1.4 720,787 + -1.9 720,790 - -0.7 720,805 - +0.1 720,826 + -0.3 720,826 + +1.1 720,866 - PGA1_c07020 0.14 -0.8 720,879 + PGA1_c07020 0.15 -0.3 720,912 + PGA1_c07020 0.19 -1.8 720,912 + PGA1_c07020 0.19 -0.5 720,914 - PGA1_c07020 0.20 -0.0 720,914 - PGA1_c07020 0.20 +1.5 720,920 - PGA1_c07020 0.20 +0.5 720,988 + PGA1_c07020 0.29 +0.4 721,037 - PGA1_c07020 0.35 +0.7 721,079 + PGA1_c07020 0.40 +1.8 721,100 + PGA1_c07020 0.42 -0.2 721,100 + PGA1_c07020 0.42 -1.7 721,100 + PGA1_c07020 0.42 -0.1 721,100 + PGA1_c07020 0.42 -0.3 721,100 + PGA1_c07020 0.42 +1.9 721,143 + PGA1_c07020 0.48 +1.8 721,151 - PGA1_c07020 0.49 -1.1 721,211 + PGA1_c07020 0.56 -0.4 721,253 + PGA1_c07020 0.61 -1.3 721,369 - PGA1_c07020 0.75 -1.1 721,459 - PGA1_c07020 0.86 -0.5 721,459 - PGA1_c07020 0.86 +0.6 721,479 + PGA1_c07020 0.88 -1.7 721,499 + +0.9 721,574 + -0.2 721,591 + -0.3 724,087 - -1.0 724,160 + -0.8 724,160 + +1.5 724,160 + -1.3 724,168 - -0.4 724,168 - +1.6 724,168 - +1.2 724,168 - +0.5 724,235 + PGA1_c07040 0.12 +0.5 724,243 - PGA1_c07040 0.13 +0.4 724,243 - PGA1_c07040 0.13 +0.5 724,244 - PGA1_c07040 0.13 -0.5 724,246 + PGA1_c07040 0.13 -0.8 724,254 - PGA1_c07040 0.14 -0.8 724,277 - PGA1_c07040 0.17 -1.1 724,333 - PGA1_c07040 0.23 -0.8 724,383 - PGA1_c07040 0.29 +0.2 724,416 + PGA1_c07040 0.32 -0.4 724,416 + PGA1_c07040 0.32 +1.1 724,416 + PGA1_c07040 0.32 +2.0 724,416 + PGA1_c07040 0.32 -1.0 724,424 - PGA1_c07040 0.33 -0.3 724,424 - PGA1_c07040 0.33 +0.5 724,424 - PGA1_c07040 0.33 +0.5 724,424 - PGA1_c07040 0.33 -1.9 724,455 + PGA1_c07040 0.37 +0.4 724,494 + PGA1_c07040 0.41 +1.3 724,494 + PGA1_c07040 0.41 +0.4 724,494 + PGA1_c07040 0.41 +1.9 724,494 + PGA1_c07040 0.41 +0.9 724,504 + PGA1_c07040 0.42 -1.1 724,530 + PGA1_c07040 0.45 -0.6 724,564 - PGA1_c07040 0.49 +0.3 724,594 + PGA1_c07040 0.53 +0.3 724,656 + PGA1_c07040 0.60 -1.1 724,675 + PGA1_c07040 0.62 -0.1 724,910 - PGA1_c07040 0.89 +1.8 724,912 + PGA1_c07040 0.89 +0.3 724,915 - PGA1_c07040 0.89 -0.4 724,918 + PGA1_c07040 0.90 +1.1 724,922 + -0.3 724,922 + +0.3 724,922 + -0.1 724,930 - -0.8 724,930 - +3.5 724,937 + -1.9 724,937 + -2.7 724,941 + +1.3 724,947 + -2.1 724,978 + -2.2
Or see this region's nucleotide sequence