Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00280 and PGA1_65p00290 are separated by 22 nucleotides PGA1_65p00290 and PGA1_65p00300 are separated by 126 nucleotides
PGA1_65p00280: PGA1_65p00280 - Protein of unknown function (DUF2793)./Domain of unknown function (DUF3476)., at 36,582 to 37,838
_65p00280
PGA1_65p00290: PGA1_65p00290 - algA: alginate biosynthesis protein, at 37,861 to 39,282
_65p00290
PGA1_65p00300: PGA1_65p00300 - GDP-L-fucose synthetase Fcl, at 39,409 to 40,398
_65p00300
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -2
-1
0
1
2 at 37.231 kb on + strand, within PGA1_65p00280 at 37.351 kb on + strand, within PGA1_65p00280 at 37.359 kb on - strand, within PGA1_65p00280 at 37.399 kb on - strand, within PGA1_65p00280 at 37.650 kb on - strand, within PGA1_65p00280 at 37.839 kb on + strand at 37.981 kb on + strand at 38.488 kb on + strand, within PGA1_65p00290 at 38.629 kb on + strand, within PGA1_65p00290 at 39.028 kb on - strand, within PGA1_65p00290 at 39.123 kb on - strand, within PGA1_65p00290 at 39.316 kb on + strand at 39.319 kb on - strand at 39.319 kb on - strand at 39.319 kb on - strand at 39.457 kb on + strand at 39.465 kb on - strand at 39.586 kb on + strand, within PGA1_65p00300 at 39.594 kb on - strand, within PGA1_65p00300 at 39.939 kb on + strand, within PGA1_65p00300 at 39.979 kb on - strand, within PGA1_65p00300 at 40.103 kb on + strand, within PGA1_65p00300
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 37,231 + PGA1_65p00280 0.52 -1.4 37,351 + PGA1_65p00280 0.61 +1.9 37,359 - PGA1_65p00280 0.62 +0.3 37,399 - PGA1_65p00280 0.65 -0.3 37,650 - PGA1_65p00280 0.85 +0.3 37,839 + +0.6 37,981 + -0.9 38,488 + PGA1_65p00290 0.44 +0.9 38,629 + PGA1_65p00290 0.54 -0.7 39,028 - PGA1_65p00290 0.82 -0.1 39,123 - PGA1_65p00290 0.89 -2.3 39,316 + -0.4 39,319 - +0.3 39,319 - -2.1 39,319 - +0.6 39,457 + -1.4 39,465 - -1.9 39,586 + PGA1_65p00300 0.18 -0.1 39,594 - PGA1_65p00300 0.19 -0.1 39,939 + PGA1_65p00300 0.54 -1.1 39,979 - PGA1_65p00300 0.58 -0.7 40,103 + PGA1_65p00300 0.70 -1.7
Or see this region's nucleotide sequence