Experiment: DinoMM minimal media with L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01220 and PGA1_262p01230 are separated by 12 nucleotides PGA1_262p01230 and PGA1_262p01240 are separated by 130 nucleotides PGA1_262p01240 and PGA1_262p01250 are separated by 74 nucleotides PGA1_262p01250 and PGA1_262p01260 are separated by 203 nucleotides
PGA1_262p01220: PGA1_262p01220 - nitric oxide reductase subunit B, at 134,078 to 135,436
_262p01220
PGA1_262p01230: PGA1_262p01230 - nitric oxide reductase subunit C, at 135,449 to 135,901
_262p01230
PGA1_262p01240: PGA1_262p01240 - hydrid cluster protein-associated redox disulfide domain, at 136,032 to 136,259
_262p01240
PGA1_262p01250: PGA1_262p01250 - transcriptional regulator, crp family, at 136,334 to 137,038
_262p01250
PGA1_262p01260: PGA1_262p01260 - copper-containing nitrite reductase NirK, at 137,242 to 138,465
_262p01260
Position (kb) 136
137 Strain fitness (log2 ratio) -1
0
1
2 at 135.313 kb on - strand at 135.446 kb on + strand at 135.454 kb on - strand at 135.454 kb on - strand at 135.473 kb on + strand at 135.746 kb on - strand, within PGA1_262p01230 at 135.897 kb on + strand at 135.903 kb on + strand at 135.910 kb on - strand at 136.009 kb on - strand at 136.153 kb on - strand, within PGA1_262p01240 at 136.225 kb on + strand, within PGA1_262p01240 at 136.298 kb on + strand at 136.304 kb on + strand at 136.304 kb on + strand at 136.317 kb on - strand at 136.364 kb on - strand at 136.582 kb on + strand, within PGA1_262p01250 at 136.610 kb on + strand, within PGA1_262p01250 at 136.814 kb on - strand, within PGA1_262p01250 at 136.950 kb on + strand, within PGA1_262p01250
Per-strain Table
Position Strand Gene LocusTag Fraction DinoMM minimal media with L-Arginine remove 135,313 - +1.2 135,446 + -0.5 135,454 - +0.3 135,454 - -0.0 135,473 + -0.4 135,746 - PGA1_262p01230 0.66 +2.4 135,897 + -0.2 135,903 + -0.4 135,910 - -0.8 136,009 - -0.2 136,153 - PGA1_262p01240 0.53 +0.5 136,225 + PGA1_262p01240 0.85 +0.7 136,298 + -0.4 136,304 + -1.0 136,304 + -1.5 136,317 - +1.2 136,364 - -1.0 136,582 + PGA1_262p01250 0.35 +0.2 136,610 + PGA1_262p01250 0.39 -0.2 136,814 - PGA1_262p01250 0.68 -1.6 136,950 + PGA1_262p01250 0.87 +0.1
Or see this region's nucleotide sequence